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L V Galebskaia

Publications and source records attributed to L V Galebskaia.

14 recordsLinked to original sources
PubMed1995

[Structure and properties of extracellular proteins regulating the proteolytic phase of complement activation].

The structure and biochemical properties of the major soluble regulatory proteins of the complement, including the CI-inhibitor, factors I, H, and J, properdin, the C4-binding protein and other protein components, are reviewed. Three types of the proteolytic complement cascade regulation by proteins are envisaged: the first one involves the inhibition of complement proteases, the second one is based on proteolytic degradation of complement activation products and the third one includes the regulation of stability of complement protein complexes. The main functional role of humoral regulatory proteins consists in the limitation of the proteolytic cascade.

Complement Activation↗
PubMed1993

[Concentration-functional interrelationships in the human alternative complement activation pathway].

The dependence of the rate of human complement mediated lysis of rabbit erythrocytes on concentrations of factors B and D of the alternative pathway is characterized by saturation kinetics. At low B and D concentrations no lysis occurs until certain "threshold" concentrations of the respective factor are reached. The type of dependence of the hemolysis rates on the C3 concentration is wavelike, i.e., at definite C3 concentrations the erythrocyte lysis is inhibited. It has been supposed that C3 not only mediates the complement-dependent hemolysis but also plays a prominent role in the self-regulation of this process.

Animals↗
PubMed1992

[Disorders of immunity in chest and abdominal injuries].

Under study were disturbances in the immune competent system in 245 patients with injuries of the chest and abdomen in dynamics of the early posttrauma period. Specific features of the disturbances were analyzed in the complicated and noncomplicated course of the trauma disease. The informative value and prognostic significance of certain indices are discussed as well as approaches to immune correction.

Abdominal Injuries↗
PubMed1988

[Pathogenetic substantiation of photomodification of autologous blood in the prevention and treatment of complications of closed chest trauma].

Three groups of patients with traumas of the chest were investigated: 1. 1533 patients treated by traditional methods: 2. 258 patients treated by transfusions of photo-modified blood; 3. 50 patients treated by autohemotransfusion. The authors have shown expediency of transfusions of photomodified autoblood. It decreased the amount of complications, lethality and improved biochemical parameters of blood.

Adolescent↗
PubMed1988

[Complications of closed chest injuries].

The role of predisposing factors in the development of complications in 1533 patients with closed traumas of the chest was studied as well as the dynamics of the state of the system of humoral regulation of microcirculation. The factors predisposing to the development of complications are the age older than 40, hospitalization later than 1 day from the trauma, double fractures of the ribs, injuries of lungs, hemothorax, concomitant diseases. In all the patients there was a subacute syndrome of disseminated intravascular coagulation, activation of the Kallikrein-Kinin system and inhibition of the complement system.

Adolescent↗
PubMedDate not supplied

[Eznzymatic conversions of ortho-nitrophenyl organophosphorus compounds in the tissues of white rats].

In homogenates of rat tissues hydrolysis of O-heptyl-O-orthonitrophenylmethyl phosphonate, O-pentyl-O-orthonitrophenylmethyl phosphonate and O-heptyl-O-isopentyl-O-orthonitrophenylphosphate was studied. Hydrolysis of the preparation was shown to be carried out mainly in liver tissue and slso in kidney and blood serum. The substances studied were cleaved only at the bond P-O-aryl. The structure of orthonitrophenyl phosphoorganic substances exhibited the distinct effect on Km and on the maximal rates of the enzymatic hydrolysis. In all the tisses studied O-heptyl-O-orthonitrophenylmethyl phosphonate was hydrolyzed with maximal rate.

Animals↗
PubMedDate not supplied

[The action of karbogal on the complement system under model conditions and in an animal experiment].

The influence of dimethylperfluorocyclohexane (carbogal) on the hemolytic activity of human blood serum complement was studied in vitro. It has been show that different effects of carbogol on the complement depend on the serum lipid composition. Endotracheal administration of carbogal to rats results in a significant reduction of complement activity in the animal's blood. Significant changes in the hemolytic activity of the complement recorded 21 days after the endotracheal administration of the compound are, probably, associated with the complement depletion after its activation by carbogal particles or with disorders in the complement component synthesis by macrophages.

Animals↗
PubMedDate not supplied

[Structure and properties of the complement system proteinases].

Biochemical aspects of enzymes of the complement functioning are presented. Proteolytic, esterolytic and amidolytic characteristics of the complement components which are enzymes in their nature as well as the structure and properties of complex proteases of the activated complement are analyzed.

Amides↗
PubMedDate not supplied

[Activity of the complement system in blood serum of dogs after treatment of acute necrotic pancreatitis with boiled pancreatic juice].

The investigations were conducted on 14 dogs. To assess the blood serum complement system, the erythrocyte lytic rate of a rabbit (RE) and a sheep (SE), the hemolytic capacity of the complement system against rabbit (RH) and sheep (SH) was measured. In acute necrotic pancreatitis, no changes in SE and SH were revealed either in the control and experimental animals. RH was decreased both in the control and experimental animals. Lower RE was found only in the controls untreated with boiled pancreatic juice. It can be suggested that in the controls the decrease in the parameter RE that characterized the activity of the complement system is associated with the emergence of complement inhibitors, which did not take place when the boiled juice was given. Preventing the complement inhibitors from forming, pancreatic juice is likely to be able to diminish the body's endogenous intoxication which is a cause of death in acute necrotic pancreatitis. This may be suggested by the higher survival of the animals and lower relative extent of pancreatic acinar tissue necrosis after boiled pancreatic juice treatment of acute necrotic pancreatitis.

Animals↗
PubMedDate not supplied

[Membrane proteins as regulators of the complement system].

The data on structure, biochemical properties and functions of membrane proteins, performing cell defence against complement lysis, are summarized. Proteins DAE (decay accelerating factor) and CR1 (complement receptor of type 1) reduce the stability of complement convertases and cause their dissociation. MCP (membrane cofactor protein) and CR1 act as cofactors. In factor I mediated proteolysis of the convertase fragments C3b and C4b. The proteins C8bp-(C8-binding protein) and protectin affect membrane attack complex assembly. In contrast to MCP and CR1, which are integrative proteins, DAF, C8bp and protectin are bound to membranes with their glycophospholypid anchors. Tissue distribution of the proteins and the ways of their solubilization into biological fluids are reviewed.

Cell Membrane↗
PubMedDate not supplied

[Novel approaches to the study of autoregulation of alternative complement pathway].

Experimental and literary data on the peculiarities of complement alternative pathway (APC) self-regulation and regulation are reviewed. There is a discussion of the methods of APC evaluation, including a new one, called complement hemolytical capacity. It is shown that the duration of complement-dependent hemolysis lag-period is limited by factor D concentration. The phenomenon of acceleration of APC-dependent hemolysis by membranes of lysed cells is described. The latter is an evidence of APC functioning in the mode of double amplification.

Animals↗