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Biomedical subjects

L V Kalakutskiĭ

Publications and source records attributed to L V Kalakutskiĭ.

At least 19 recordsLinked to original sources
PubMed1990

[Study of the optimal conditions of preservation of cephalosporin C-producing fungus Acremonium chrysogenum].

The influence of various preservation conditions of viability and antibiotic activity of Acremonium chrysogenum 281A and 305A strains producing cephalosporin C was studied. Cryogenization of the culture in the form of suspension of the vegetative mycelium in 20 per cent glycerol solution at a rate of 1 degree/min showed to be advantageous over lyophilization and L-drying. Cryogenization under such conditions provided rather high viability of the culture and preservation of its initial antibiotic activity for the period of its storage for at least 1.5 years in liquid nitrogen at a temperature of -196 degrees C.

Acremonium↗
PubMed1983

[Growth and antimicrobial activity of an association of an actinomycete and a green alga].

During joint cultivation of the actinomycetes Streptomyces griseus (strains 65 and 744) isolated from the soil and the green algae Chlorella vulgaris larger amount of biomass as compared with solitary axenic culture have been shown. The relation of biomass of actinomycetes S. griseus strain 65 and S. griseus strain 744 and algae in the lichen-like experimentally formed thallom make up 42:1 and 40:1 relatively, i. e. the mass of actinomycetes forms 97-98% from the mass of thalloms. Actinomycetes in the associations with the algae accumulate larger amount of biomass that in the axenic cultures on corresponding medium, whereas the algae produce the same amount of the biomass as the axenic culture under the same conditions. The associations have the antimicrobic properties differed from the axenic cultures established.

Antibiosis↗
PubMed1976

[Conditions for the self-organization of submicroscopic structures from mycelial extracts of the "fructose" variant of Actinomyces roseflavus var. roseofungini].

Self-organization of various submicroscopic formations, such as "tubes", "ribbons", etc. differing in ultrastructure and containing a polyenic antibiotic was observed in acetone extracts of the mycelium of the "fructose" variant of Actinomyces roseoflavus var. roseofungini. Conditions for separate isolation of the above structures are described. Concentration of the extract and the ratio of the volumes of water added to the extract are determinant for the process of self-organization. A possibility of reorganization of the tubular structures into the ribbon-like ones and vice versa is shown.

Cell Fractionation↗
PubMed1976

[Production of tubular structures from the aerial mycelium of Actinomyces roseoflavus var. roseofungini].

Tubular formations analogous by their structure to those found on the surface of the outer sheath of the hyphae of the aerial mycelium of Actinomyces roseoflavus var. roseofungini were reconstructed in vitro from the mycelium acetone extract on addition of water. A polyenic antibiotic was shown to be present in the composition of the "tubes". A possible role of the antibiotic in the genesis of the secondary structures of the actinomycete is discussed.

Culture Media↗
PubMedDate not supplied

[Reparation of viability of arthrospores subjected to boiling in Actinomyces streptomycini].

Endospores of Streptomyces streptomycini B-16 are considerably more thermostable (by 40degreesC) than it was generally accepted. The bulk of populations of the spores (up to 55%) can be subjected to 100degreesC during 10 min but does not germinate under usual conditions as a result of heat damage which may be repaired at a relative humidity of 100% or in water suspensions at temperatures optimal for growth of the actinomycete. The rate of reparation increase upon washing of the heated spores with stirring, centrifuging and especially after treating the spores with ultrasound. The treatment is supposed to eliminate from the spores growth inhibitors which were formed as a result of heat damage. Characteristics of the process are discussed, which make it different from other reparation systems described elsewhere, in particular its endogenous character.

Actinomyces↗
PubMedDate not supplied

[Pigment and antibiotic substance contained in the spores of Actinomyces streptomycini B-6].

The antibiotic activity and fractional composition of the supernatant of the homogenate of Actinomyces streptomycini B-6 spores were investigated. The homogenate supernatant was of yellow colour and showed antibiotic activity when used as the test-organism of the culture St. aureus 209. The antibiotic activity was associated not with the homogenate pigment but with the colourless substance referring to streptomycins which was contained in spores.

Actinomyces↗
PubMedDate not supplied

[Melanin pigment of Actinomyces umbriferus].

Properties of a dark-brown pigment complex isolated from the spores, mycelium and cultural broth of Actinomyces umbriferus by a conventional technique were compared. The complex consists of three fractions: one is a true melanin while two other fractions differ by several characteristics from melanins and from each other. The fraction which is most similar to melanin prevails in the spores, is contained, in a relatively small amount, in the mycelium, and is absent from the cultural broth. Melanin isolated from the spores has a low relative content of carbon and nitrogen and a higher ash content as compared to melanin isolated from the vegetative mycelium. The pigment complex of the spores is characterized also by differences in properties of the first isolated fraction. The pigment complex from the cultural broth differs from the complex of the mycelium and spores by properties of the studied fractions.

Actinomyces↗
PubMedDate not supplied

[Development of Thermoactinomyces vulgaris on a synthetic medium].

A synthetic medium was used to obtain the dormant spores of Thermoactinomyces vulgaris. The fraction of the dormant spores depended on the amino acid composition of the growth medium. The rate of growth and development of the organism on the synthetic medium is lower as compared to the routinely employed complex medium.

Culture Media↗