Biomedical subjects

L V Kolombet

Publications and source records attributed to L V Kolombet.

3 recordsLinked to original sources

PubMed1978

[Comparative study of the changes in lactate dehydrogenase isoenzymatic spectra in the course of organogenesis in mice].

The dynamics of changes in the lactate dehydrogenase (LDH) spectra during organogenesis in CBA mice has been studied by means of ultramicroelectrophoresis. The embryonic period of development is characterized by the predominance of cathodic isozymes (LDH-5 and LDH-4) in all the organs under study. The increase of anodic isozymes (LDH-1 and LDH-2) takes place in the heart, kidneys and brain as the development proceeds. The first reliable differences in the LDH spectra of different organs appear on the 11th day of embryogenesis. On the basis of comparison with the help of criterion gamma, the LDH spectra of the organs under study can be divided into two groups: I--heart, lungs, kidneys and brain (tendency towards the increase of H-subunits) and II--intestine, liver and muscles (tendency towards the increase of of M-subunits). The LDH spectra of adult animals are divided into 4 distinct groups: I--heart and kidneys, II--brain, III--lungs and muscles, IV--liver.

Animals↗

PubMed1977

[Repression and derepression of lactate dehydrogenase loci during mouse development].

The ultramicroelectrophoretic method was applied to the study of the lactic dehydrogenase (LDH) spectrum alterations during the developmental phases of mice: ovulated ova--zygote--blastocyte--embryo--oocyte--ovulated ova. Only H-subunits were found in the embryo cells before the 5th day of development. After this M-subunits appeared indicating derepression of LDH-A locus. On the 8th day of the embryonal development deres pression of the LDH-B locus was observed to disappear during the oogenesis, being the result of progressive repression of locus LDH-A. Dictiotena of meios prophase is characterised by active H-subunit synthesis and a gradual decrease of the M-subunit synthesis. During the whole dictiotena phase the LDH-spectrum of the follicular cells was of the M-type.

Animals↗

PubMedDate not supplied

[Microfungicid--a preparation based on trichoderma viride for plant diseases control].

A technology was designed for manufacturing a preparation based on Trichoderma viride Pers ex S.F. Gray that strongly suppresses the development of causative agents of certain plant diseases and displays a growth-stimulating activity. Cultivation of the strain in a liquid medium for 18-24 h produced up to 60 g dry biomass per liter nutrient medium. A marketable form created in this work conserves the activity of the mycelial preparation for six months. The preparation is compatible with insecticides (carbofos, vismetrin, talstar, and applaud) and certain fungicides (such as baitan). Tests performed with the liquid form of Mycofungicid (seeds were treated with this preparation at a dose of 20-30 g per metric ton before sowing) showed its high efficiency in protecting cereal crops from plant pathogens. The incidence of plant diseases decreased by 65%, and crop yields increased by 15-20%.

Fungicides, Industrial↗