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L V Moore

Publications and source records attributed to L V Moore.

24 records · Page 2Linked to original sources
PubMed1987

Effect of high-fiber and high-oil diets on the fecal flora of swine.

Six pairs of pigs were fed a basal diet, a high-fiber diet, and a diet high in corn oil in different sequences to minimize the carry-over effect of diet. After 2 months on each diet, a fecal specimen from each pig was cultured on nonselective medium in roll tubes. Fifty colonies were randomly selected from each fecal sample, and isolates were characterized to identify a representative cross section of the fecal flora. The bacterial composition of the fecal flora differed between basal and high-fiber diets (P = 0.002) and between high-fiber and high-oil diets (P = 0.015). However, the floras were not significantly different between the basal and the high-oil diets (P = 0.135), nor were the floras of the 12 individual pigs (each on all three diets) statistically different (P = 0.103). Only 14 of the 160 observed taxa have been detected in the human fecal flora, and only 159 of 1,871 total isolates (8.5%) were members of described species. The most common isolate was a Streptococcus species similar to that reported by Robinson et al. (I. M. Robinson, S. C. Whipp, J. A. Bucklin, and M. J. Allison, Appl. Environ. Microbiol. 48:964-969, 1984), which was found in 34 of 36 samples and which represented 27.5% of all isolates. Lactobacillus, Fusobacterium, Eubacterium, Bacteroides, and Peptostreptococcus species were the next most common bacteria. Escherichia coli represented 1.7% of all fecal isolates, which is somewhat higher than the 0.1 to 0.6% observed in human feces cultured similarly with prereduced anaerobically sterilized media.(ABSTRACT TRUNCATED AT 250 WORDS)

Animal Feed↗
PubMed1987

Bacteriology of human gingivitis.

The subgingival bacterial floras of naturally occurring gingivitis in adults and children were characterized and compared with the floras of other periodontal conditions previously studied. The composition of the gingivitis floras was found to be distinct from that of floras associated with health or with moderate, severe, or juvenile periodontitis. There were no major differences between the floras of naturally-occurring gingivitis and the floras of the human experimental gingivitis model. Data indicated that the flora of healthy sites within a mouth is influenced by the number of inflamed sites, which argues against independence of sites bacteriologically. Proportions of ten bacterial species increased in both gingivitis and periodontitis, as compared with health, in both adults and children. These species were found in both affected and unaffected sites of people with gingivitis. The numbers of five other cultivable species and the "large treponeme", which was not cultivated, increased in gingivitis and periodontitis of adults only. Significant differences in non-spirochetal floras between children and adults were not found, although they were in the experimental gingivitis model studied previously. Cultivable spirochetes did differ between children and adults. Children had fewer samples positive for spirochetes, and children's positive samples contained greater proportions of T. socranskii subsp. paredis. Some species that predominate in periodontitis, but which are absent from healthy gingivae, were found as a small percentage of the flora in gingivitis. This suggests that increased serum and blood in the gingival crevice encourage species that relate to periodontitis.

Adult↗
PubMed1986

Use of the RapID-ANA System to screen for enzyme activities that differ among species of bile-inhibited Bacteroides.

The RapID-ANA System (Innovative Diagnostics Systems, Inc., Atlanta, Ga.) was used to test 102 strains of 14 species of phenotypically similar bile-inhibited Bacteroides from humans. Bacteroides oris, Bacteroides veroralis, Bacteroides buccalis, Bacteroides melaninogenicus, Bacteroides loescheii, and Bacteroides denticola had very similar enzyme activity profiles. Clear differentiation of these six species by the RapID-ANA System was not possible, but tests for arginine aminopeptidase and beta-glucosidase were helpful. Bacteroides oralis, Bacteroides intermedius, Bacteroides corporis, Bacteroides disiens, Bacteroides bivius, Bacteroides gingivalis, Bacteroides asaccharolyticus, and Bacteroides buccae each had unique enzyme activity profiles. No consistent differences in enzyme activities were found between the two DNA homology groups within Bacteroides melaninogenicus, Bacteroides loescheii, or Bacteroides intermedius. Tests for glycine aminopeptidase, alpha-galactosidase, arginine aminopeptidase, alpha-fucosidase, N-acetylglucosaminidase, reduction of triphenyltetrazolium, and production of indole were helpful in the differentiation of the species studied.

Acetylglucosaminidase↗