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Biomedical subjects

L V Panasiuk

Publications and source records attributed to L V Panasiuk.

14 recordsLinked to original sources
PubMed1975

[A pharmacologic analysis of several adrenomimetic effects of serotonin].

Contractions of the isolated rat vas deferens in response to the addition of serotonin could be explained by the release of catecholamines from the nerve endings. As shown by the use of D-, M- and T-antagonists of serotonin (LSD-25, indocarb, typindole), symatholytic--bretilium, alpha-adrenolytic--droperidol and imipramine, this effect was not associated with the direct activation of serotonin- and adrenoreactive receptors.

Animals↗
PubMed1975

[Adrenergic component in the mechanism of action of sodium oxybutyrate].

The influence of sodium oxybutyrate on the adrenergic neurotransmitter content in the nerve fibers and the synaptic vesicles and the uptake of exogenous noradrenaline (NA) by the sympathetic nerves of rat Vas deferens was studied by spectrofluorimetry, fluorescent-histochemistry and cytochemical electron microscopy. Sodium oxybutirate failed to influence the stores of the adrenergic mediator, but was capable of blocking the uptake and accumulation of the exogenous NA.

Animals↗
PubMed1975

[Effect of carbidine on the process of adrenergic neurotransmission].

Methods of physiological analysis were applied to a study of the effect of an original psychotropic agent--carbidine--on the process of adrenergic neurotransmission on a model of an isolated rat Vas deferens. The noradrenaline (NA) content was determined in the same ductus spectrofluorimetrically; the capacity of the neuroleptic to block the consumption of the exogenous NA by the tissue was also investigated. Carbidine proved to possess an adrenomimetic effect caused by it capacity to release NA, leading to a reduction of endogenous neuromediator stores.

Animals↗
PubMed1975

[Influence of nonakhlazin on adrenergic neurotransmission in the vas deferens of rats].

The effect of a new antianginal drug--nonachlazine on the adrenergic neurotransmission in the isolated rat vas deferens was studied by examining the vas deferens contractions in response to the transmural electric stimulation of the postganglionic sympathetic nerves and addition of noradrenaline (NA) or BaCl2. After the nonachlazine treatment the NA content in the vas deferens was also studied by the spectrofluorometric method. Besides, the effect of the drug on the uptake of the exogenous NA was investigated. Nonachlazine was found to possess some sympatholytic and spasmolytic effect and could block the uptake of the exogenous NA greatly.

Animals↗
PubMed1975

[Effect of carbidine on the content and storage of adrenergic neurotransmitter in the synaptic vesicles].

The influence of carbidine, an original psychotropic drug, on the adrenergic neurotransmitter content and storage in the sympathetic nerves was studied with the use of cytochemical electron microscopy. The influence of carbidine on the uptake of the exogenous noradrenaline (NA) in the synaptic vesicles was also studied. Carbidine was found to be capable to decreasing the NA storage in the synaptic vesicles and failed to block the accumulation of the exogenous NA in the synaptic vesicles.

Amines↗
PubMedDate not supplied

[Effect of aminazine, tryptazine and droperidol on the uptake and accumulation of exogenous noradrenaline].

Spectrofluorometry, fluorescent histochemistry and cytochemical electron microscopy were employed in studying the capacity of the neuroleptics trifluoroperazine, chlorpromazine and droperidol to bar the capture and accumulation of exogenous norepinephrine (NE) in the tissue, of adrenergic nerve fibers and synaptic vessels of an isolated Vas deferens of the rat. Trifluoroperazine and droperidol were found to be devoid of the property to inhibit the capture and accumulation of NE in the tissue, nerve fibers and in the synaptic vesicles. Chlorpromazine blocked the accumulation of the neurotransmitter, when the mediator was added in a concentration of 1 gamma/ml and failed to produce an inhibitory effect, when NE was used in a greated concentration (30 gamma/ml). It is concluded that all the studied neuroleptics do not hinder the neurotransmitter gaining access into synaptic vesicles, when it is accumulated in the axoplasma.

Amitriptyline↗
PubMedDate not supplied

[Effect of nonachlazine on the processes of uptake and release of nordrenaline].

Nonachlazine (2 micrometer) blocked significantly the uptake 1 of endogenic noradrenaline in the myocardial tissues and in adrenergic neurons of vas deferens. The uptake II, as well as the intensity and dynamics of the noradrenaline release were not affected by nonachlazine. These processes resulted in an increase of free noradrenaline concentrations in the synaptic space. The latter seems to be a result of the mediator's passage into the nerve fibers (through axonal membranes) and into the granule, this being due to the nonachlazine-induced block. It is suggested that the metabolic and functional nonachlazine effects are related to the beta-adrenergic receptors stimulation, mediated through the agency of noradrenaline.

Adrenergic Fibers↗
PubMedDate not supplied

[Ability of adrenergic nerves to accumulate exogenous noradrenaline under some pharmacological influences].

By employing spectrofluorometry and quantitative fluorescent histochemistry the norepinephrine (NE) content in the tissue and in the adrenergic nerves of Vas deferens of the rat after depletion of the transmitter's reserves with tyramine and their subsequent replenishment through incubation of the tissue with exogenous NE was measured. The object of investigations was also the influence of antidepressants (phthoracizine and imipramine) and of the cholinolytic spasmolytin (diphacyl) on processes of the NE accumulation. Phthoracizine, impramine and spasmolytin were found to capable of inhibiting accumulation of the exogenous NE in the tissue and in the adrenergic nerves of Vas deferens, provided the mediator is present in the extraneuronal medium in a concentration of 0.5 gamma/ml. When the neurotransmitter was introduced into the medium in higher concentrations (10 gamma/ml) the antidepressents and cholinolytic did not produce the said effect. A correlation of spectrofluorometric and quantitative fluorescent-histochemical findings was observed in cases when the tissue contained less than 50% (i.e. less than 9 gamma/ml) of the total NE reserves.

Animals↗
PubMedDate not supplied

[Effect of neuroleptics on spontaneous and evoked noradrenaline release from brain nerve endings of rats].

Spectrofluorometric determination of the noradrenaline content in the non-purified fraction of the rat's brain stem synaptosomes and radioisotope method resulted in finding that carbidine is capable to release noradrenaline from the nerve endings. Azabutyron, droperidol and fluphenazine had no noticeable effect on this process. The release of noradrenaline with carbidine depends upon the presence of Ca2+ ions in the medium. This neuroleptic is also capable of intensifying the release of the mediator provoked by the potassium depolarization of the nerve endings.

Animals↗
PubMedDate not supplied

[Neuromediator content in the synaptic vesicles of rat adrenergic nerves in some pharmacological actions].

Cytochemical electron microscopy was employed to study the content of neurotransmitters in the synaptic vesicles of adrenergic nerve fibers of Vas deferens of the rat following depletion of the meadiator's reserves by tyramine and subsequent accumulation of exogenous norepinephrine. Subject to investigation was also the effect of antidepressants (phthoracizine and imipramine) on the accumulation of exogenous norepinephrine in the synaptic vesicles. Phthoracizine and imipramine (1 and 10 gamma/ml) are shown to block the acculation of norepinephrine in the vesicles, when the mediator is introduced in a concentration of 0,5 gamma/ml, and not to impede this process, if the mediator's concentration is increased to 30 gamma/ml.

Animals↗
PubMedDate not supplied

[Influence of carbidine on the noradrenaline level in the adrenergic nerve fibers in the peripheral tissues].

By employing fluorescent histochemistry and spectrofluorimetry the influence of an original psychotropic substance -- carbidine on the level, localization and accumulation of the adrenergic mediator in Vas deferens, dura mater and the kidney capsule was studied "in vivo" and "in vitro". Carbidine was shown to capable of liberating the adrenergic mediator from the sympathetic nerve fibres of the peripheral tissues without affecting the capture and accumulation of exogenous norepinephrine by adrenergic nerve fibres.

Adipose Tissue↗