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Biomedical subjects

L Vajrabhaya

Publications and source records attributed to L Vajrabhaya.

7 recordsLinked to original sources
PubMed1997

Comparison between Sulphorhodamine-B dye staining and 51Cr-release method in cytotoxicity assay of endodontic sealers.

The aim of this study was to evaluate Sulphorhodamine-B (SRB) staining against 51Cr-release in cytotoxicity tests of six endodontic sealers, namely, MU sealer (Mahidol University) ROCANAL 2, ROCANAL 3, Apexit, Endomethasone, and AH-26. Monolayers (5 x 10(5) cells/ml) of the mouse cell line Mu-mu-1 were used as test cells. Following incubation at 37 degrees C in 5% CO2 for 24 h in the presence of each sealer, cells were stained with 0.4% SRB and the absorbance at 540 nm determined as measure of cell viability. For 51Cr-release assay, cells were labelled with 51Cr before testing with sealers, and radioactivity in the supernatant was measured in a liquid scintillation counter. Both techniques indicated that Apexit was the least toxic sealer. In view of the ease of conducting SRB staining for tests of cell viability, this may be the method of choice over 51Cr-release assay in the evaluation of endodontic sealer cytotoxicity.

Analysis of Variance↗
PubMed1997

Multilayer and monolayer cell cultures in a cytotoxicity assay of root canal sealers.

The aim of this study was to evaluate the response of a multilayer compared with a monolayer cell culture using six root canal sealers. Both monolayer and multilayer of MU-mu-1 (Mahidol University mouse cell line 1) were cultured in separate 96-well plates. Following incubation at 37 degrees C in 5% CO2 for 4 h in the presence of each sealer, cells were stained with 0.4% Sulphorhodamine-B, viable dye staining and the absorbance at 540 nm determined and calculated as cell viability. There was no statistical difference in the percentage viability for each sealer in both the multilayer and the monolayer cell culture (P > 0.01). Apexit and AH-26 were less toxic (P < 0.01) than MU (Mahidol University) sealer, ROCANAL 3, ROCANAL 2, and Endomethasone which demonstrated the same toxicity (P > 0.01).

Animals↗
PubMed1997

Accuracy of apex locator.

An evaluation of the accuracy of the "Root ZX" apex locator was conducted on 20 single-rooted teeth that had been extracted because of severe disease or orthodontic treatment. After the pulp chamber had been accessed and the tooth length determined using the Root ZX apparatus, the file was fixed to the tooth with light-cured composite resin. After extraction, the tooth was longitudinally ground with a diamond bur until the file was visible. The distance between the file tip and the apical foramen was measured under a microscope (x 100). The results showed that the tip of the instrument reached the apical foramen in all teeth.

Adult↗
PubMed1989

Nonsurgical endodontic treatment of a tooth with double dens in dente.

A case of "double" dens in dente with an open apex and periapical lesion is reported. Nonsurgical endodontic treatment was performed. The canals were medicated for 5 months with calcium hydroxide before obturation with combined sectional and thermoplasticized warm gutta-percha. Follow-up revealed an asymptomatic patient and a reduction in size of the periapical lesion.

Adult↗
PubMedDate not supplied

[A case report of root resorption treatment].

A case report of endodontic surgery of maxillary left lateral incisor with external root resorption which perforated the root canal was presented. The cause of this abnormality was due to chronic inflammation. The patient had the history of pain and swelling. After root canal filling by thermoplasticized warm gutta-percha technique, the resorption defect and the apical apex were filled with amalgam via a surgical approach. The patient remained asymptomatic 8 months later, radiographic appearance revealed a sign of bone repair.

Gutta-Percha↗