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Biomedical subjects

L Volterra

Publications and source records attributed to L Volterra.

At least 19 recordsLinked to original sources
PubMed1992

Microcystin-like toxins in different freshwater species of Oscillatoria.

In January and September of 1989 and March 1990 blooms of Oscillatoria rubescens, Oscillatoria tenuis and Oscillatoria mougetii were found in Lake Simbirizzi and Lake Flumendosa in Sardinia, and in Lake San Puoto in the Lazio region of Italy. By using different extraction methods and HPLC analysis, two microcystin-like toxins (RR-like and YR-like), similar to some of the toxic compounds produced by the Cyanophycea Microcystis aeruginosa, were detected in these blooms.

Chromatography, High Pressure Liquid↗
PubMed1990

Presence of saxitoxin in toxic extracts from Gonyaulax polyedra.

A "red tide" bloom of Gonyaulax polyedra occurred in Italy in Autumn, 1988. Algal concentrated extracts and undiluted water samples from the bloom were tested both with the Microtox system and a mouse bioassay, revealing the presence of paralytic shellfish poison-like neurotoxins. Saxitoxin levels evaluated on the basis of toxicological and instrumental analysis showed discrepancies. Other toxins could be present in addition to paralytic shellfish poison.

Animals↗
PubMed1990

Identification of enteroviruses isolated from sea-water: indirect immunofluorescence (IIF).

In this research the Enteroviruses presence in sea water was studied. In previous studies presumptive Enteroviruses were revealed in 43% and in 77% of sea water samples analyzed. It was necessary to identify viral particles isolated from marine water because the detection of this kind of virus was performed only on the basis of cytopathic effect appearance on cell cultures. The aim of this research was to verify the suitability of Indirect Immunofluorescence for identification of presumptive Enteroviruses isolated from marine waters. 13 field strains from RC 37 cells (Cercopithecus Kidney cell line) were tested. Pools of Horse immune sera against COXS, POLIO and ECHO, and anti-horse antibodies conjugated with fluorescein were used. The results revealed the presence of Coxsackie virus and Echo virus. There were many problems related with ECHO identification for the presence of cross-reactions with COXS. The IIF method cannot be performed routinely, because it did not show high level of specificity and sensibility, and it is expensive. This methodology can be used only in particular cases and in laboratories with competent virologists and related facilities. In the future, the only suitable methodology for identification of Enteroviruses isolated from field samples is based on the use of RNA and DNA probes.

Antigens, Viral↗
PubMed1990

[Comparative evaluation of Escherichia coli strains as markers for the study of bacteriophages].

The count of coliphages in polluted waters was found to be dependent on many experimental factors. The host-strain used for their enumeration is among the most important. In this paper we report a comparative investigation on the variability of counts of coliphages in sewage as a result of variations in host-strains of Escherichia coli and in methodologies. Two methods were used for enumerating them: the M.P.N. and the direct count. E. coli C, B1, Hfr and E36 consistently produced more plaques than any other host tested.

Blood Coagulation Factors↗
PubMed1979

The Ligurian coast: an example of data processing.

An attempt of computer data processing carried out on microbiological analysis of marine coastal waters is reported in the present paper. The Ligurian coast was chosen because of the extensive analyses executed by the Laboratori Provinciali di Igiene e Profilassi. These have supplied us with their results concerning the marine environment. The elaboration performed discriminates between those stations constantly polluted and the punctual pollution phenomena that seldom affect more than one station. Data obtained could be utilized for a correct management of the coast and of the adjacent land.

Computers↗
PubMed1979

Shellfish quality standards.

Total coliforms, faecal coliforms, enterococci and heterotrophic bacteria were determined in shellfish samples collected both in the marine environment (at Bacoli and Coroglio in the Bay of Naples) and from the local market. The same bacteriological analyses were carried out on water samples obtained from the shellfish growing areas. The results are considered in relation to standards applied in Italy and elsewhere to shellfish and shellfish cultures.

Animals↗
PubMed1975

Presence of two virus-like particles in Penicillium citrinum.

Two icosahedral virus-like particles (28 and 19 nm in diameter, respectively) have been detected in sporogenic and asporogenic segregants of a strain of Penicillium citrinum. The distribution of the two particles differed among the two segregants.

Microscopy, Electron↗
PubMedDate not supplied

Diarrhoeic shellfish toxins in Adriatic Sea mussels evaluated by an ELISA method.

A competitive enzyme immunoassay (ELISA) was used to determine the presence of okadaic acid (OA) and/or dinophysistoxin-1 (DTX-1) in mussels. The accuracy and sensitivity of the ELISA method has been checked. The sensitivity of the method (100 ng/g of hepatopancreas) makes it possible to determine OA concentrations ten times lower than the tolerance limits established by the Health Authorities of many countries. For the first time, OA and/or DTX-1 were detected in mussels (Mytilus galloprovincialis) collected in different stations along the Adriatic Sea coasts.

Animals↗