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Biomedical subjects

L Walter

Publications and source records attributed to L Walter.

At least 37 records · Page 2Linked to original sources

The determinants of fat intake in a multi-ethnic New Zealand population. Fletcher Challenge--University of Auckland Heart and Health Study Management Committee.

BACKGROUND: The New Zealand diet is high in total and saturated fat and this is likely to be contributing to the increasing prevalence of obesity and relatively high rates of coronary heart disease in New Zealand. The identification of subgroups with a high-fat intake will enable nutrition-related public health strategies to be better targeted. METHODS: Subjects from two surveys were included in the study: 7574 employees from a large multinational workforce survey and 2447 people aged 35-84 years selected from a stratified random sample of the electoral roll in central Auckland. Fat and saturated fat intake were assessed by short questionnaire which gave a dietary fat habits (DFH) score and supplemented by a six-item food frequency questionnaire. RESULTS: The DFH scores were higher in males than in females at all ages, and there was an inverse relationship with age which was stronger for males. Age-adjusted scores showed significantly higher DFH scores for Maori than for Europeans. Lower socioeconomic status was associated with higher DFH scores in males. Current smoking and heavy drinking (in males) were associated with significantly higher DFH scores after controlling for socioeconomic status. The results of the limited food frequency questionnaire supported the trends in DFH scores. CONCLUSIONS: The subgroups with high total and saturated fat intakes which should be a priority for public health action are young and middle-aged males, Maori and lower socioeconomic status males. The clustering of high-fat intake with smoking and heavy drinking should be considered when developing preventative strategies.

Adult↗

Physical mapping of the Ring1, Ring2, Ke6, Ke4, Rxrb, Col11a2, and RT1.Hb genes in the rat major histocompatibility complex.

A contig of cosmids containing the Ring1, Ring2, Ke6, Ke4, Rxrb, and Col11a2 genes in the rat major histocompatibility complex has been established and aligned to the class II cluster (RT1.Hb). The relative order and the transcriptional orientations of these rat genes are the same as in their human and mouse homologues. The distances are also similar, except for the Col11a2 - class II interval, which is clearly shorter in rat and mouse compared with human. The previously defined Ke5 probe could be shown to map into the large Col11a2 gene.

Amino Acid Sequence↗

Chromosome mapping of rat histone genes H1fv, H1d, H1t, Th2a and Th2b.

Chromosome assignment of the rat histone genes H1t, H1d (H1.4), H1fv (H10), Th2a and Th2b is described. The testicularly expressed histone genes H1t, Th2a and Th2b could be assigned to rat chromosome (RNO) 17 by PCR analysis of somatic cell hybrid DNAs. The H1d gene was mapped to RNO17p12-->p11 by FISH. These genes might form a histone gene cluster homologous to that found on HSA6p21.3 in humans and MMU13A2-3 in mice. The rat histone H1fv gene was assigned to RNO7 by PCR. This result allows the inclusion of rat H1fv to an established conserved group of syntenic genes in rat, mouse and human on chromosomes RNO7, MMU15 and HSA22, respectively.

Animals↗

Identification of a novel conserved human gene, TEGT.

A novel gene, TEGT (testis enhanced gene transcript), has been identified in humans. It does not belong to any known gene family of vertebrates. The deduced amino acid sequence of the gene and a bacterial protein of unknown function show low but significant homology and very similar hydrophobicity profiles. Two different transcripts of TEGT occur, which are due to alternative usage of two polyadenylation sites. The presence of a nuclear targeting motif indicates that the gene product might localize to the nucleus. The TEGT gene maps to human chromosome 12q12-q13 and belongs to a syntenic group, which is conserved in human, mouse, and rat.

Animals↗

Characterization and mapping of a highly conserved processed pseudogene and an intron-carrying gene of the heat shock cognate protein 70 (Hsc70) gene family in the rat.

A processed pseudogene of the rat Hsc70 gene, Hsc70-ps1, is described, which still presents the open reading frame of the original gene. The pseudogene does not appear to be expressed. It maps to rat Chromosome (Chr) 2. The intron-carrying Hsc70 gene localizes to Chr 8. Hsc70-specific probes detect a large number of more than 20 cross-hybridizing fragments, which show only limited length polymorphism among various inbred rat strains.

Animals↗

Genetic aspects of the hsp70 multigene family in vertebrates.

The family of genes encoding heat shock proteins of about 70 kDa (hsp70) in vertebrates is reviewed under genetic aspects. After a detailed description of the various hsp70 genes more general characteristics of the organization and evolution of the multigene family are discussed.

Amino Acid Sequence↗

A novel, conserved gene of the rat that is developmentally regulated in the testis.

From a rat testis library three overlapping cDNA clones were isolated that represent a novel single-copy gene, designated Tegt. Two transcripts of 2.8 and 1.0 kb were found in each organ tested. The shorter transcript was highly abundant in adult testis. A similar expression pattern was found in the mouse. Analysis of rat RNA from different stages of spermatogenesis indicated that accumulation of the short transcript occurred mainly postmeiotically. The rat Tegt gene maps to Chromosome (Chr) 7, and its mouse homolog to Chr 15.

Aging↗

Comparative analysis of the three major histocompatibility complex-linked heat shock protein 70 (Hsp70) genes of the rat.

The organization of the three major histocompatibility complex (Mhc)-linked heat shock protein 70 (Hsp70) genes Hsp70-1, Hsp70-2, and Hsp70-3, and the nucleotide sequences of these genes, are presented for the rat. Hsp70-1 and Hsp70-2 gene products are identical at the amino acid level. From the pattern of sequence similarity of the orthologous Mhc-linked Hsp70 genes of rat, human, and mouse, it is concluded that the gene duplications leading to the three-gene cluster occurred before the separation of the primate and rodent lines and that the Hsp70-1 and Hsp70-2 genes of rat and human might have undergone homogenization of their sequences.

Amino Acid Sequence↗

Restriction fragment length polymorphism of the major histocompatibility complex-linked heat shock protein 70 (Hsp70) genes of the rat.

DNAs from inbred rat strains representing eighteen major histocompatibility complex (RT1) haplotypes have been tested for restriction fragment length polymorphisms of the three heat shock protein 70 (Hsp70) genes mapping into this complex. The DNAs were digested with restriction enzymes BamHI, EcoRI, PstI or PvuII and assayed by probes hybridizing to all three genes or selectively to Hsp70-1, Hsp70-2 and Hsp70-3, respectively. Three to four alleles were detected with each enzyme and in total five different strain distribution patterns could be found. Neither deletions nor amplifications of genes were detected in the three-gene Hsp70 cluster.

Animals↗

Comparison analysis of illicit cocaine samples.

A rapid method for comparison analysis of illicit cocaine samples has been developed. The raw data are obtained by capillary gas chromatography using a nitrogen-phosphorus detector. The area ratios of four alkaloids (tropacocaine, norcocaine, cis-cinnamoylcocaine, and trans-cinnamoylcocaine) to cocaine are calculated for each sample. These ratios are compiled in a computer database which allows easy comparison of samples and makes possible reliable conclusions regarding their commonality of origin.

Chromatography, Gas↗

A cluster analysis of the multidimensional pain inventory.

Seventy-nine patients with chronic headaches of diverse causes, recruited from a headache clinic's biofeedback facility, were administered the Multidimensional Pain Inventory (MPI) for measuring the cognitive, behavioral, and affective dimensions of pain. Using the statistical technique of cluster analysis to organize the results, three clusters emerged, and were similar in their characteristics to those named "Dysfunctional", "Interpersonally Distressed", and "Adaptive Coper" by other authors who had applied the Inventory and the cluster analysis technique to other populations (one population containing heterogenous groups of chronic pain patients, and another population of patients suffering from "temporomandibular joint disorders". Additional analyses of our results confirmed that the three groups were distinct from one another; and that age, sex, duration of complaint, and diagnosis, were not factors in the formation of the groups. Our results suggest that the MPI is a valid measure of the cognitive, behavioral and affective aspects of pain. Rather than apply a similar intervention program to all headache patients, it might be more effective to tailor treatment to the variations in these aspects exhibited by patients in the three different clusters.

Adolescent↗