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L Wan

Publications and source records attributed to L Wan.

81 records · Page 5Linked to original sources
PubMedDate not supplied

Rapid T2-weighted MR imaging of uterine leiomyoma and adenomyosis.

BACKGROUND: To compare three rapid T2-weighted pulse sequences with high-resolution turbo spin-echo (SE) magnetic resonance (MR) imaging for the diagnosis of leiomyoma and adenomyosis. METHODS: Eighteen patients referred for evaluation of suspected leiomyoma or adenomyosis underwent imaging at 1.5 T with a phased-array multicoil. Non-breath-hold, fat-saturated sagittal images of 4-7 mm, with equivalent voxel size, were obtained through the pelvis with the following three rapid pulse sequences: segmented, half-Fourier single shot turbo SE (HASTE), turboGRASE (TGSE) and turbo SE MR images. Mean acquisition times were 17 s (HASTE), 37 s (TGSE), and 42 s (turbo SE). These images were compared, in a blinded fashion, to high resolution turbo SE MR images, which are considered the "standard" for pelvic MRI. RESULTS: The three rapid pulse sequences, HASTE, TGSE and turbo SE, provided equivalent diagnostic information when compared with high-resolution turbo SE MR images. There was no significant difference in image quality, detection and localization of leiomyoma or in diagnosis of adenomyosis among the three rapid sequences. HASTE imaging demonstrated the least ghosting. CONCLUSION: Diagnostic T2-weighted images of benign uterine pathology may be obtained in as little as 17 s.

Adult↗
PubMedDate not supplied

The legality of unlinked anonymous screening for HIV infection: the U.S. approach.

The purpose of this article is to describe the legal approach, and in particular, the specific statutory vehicle adopted by the U.S. with respect to unlinked anonymous screening, and to offer the U.S. approach as a possible model for those countries presently considering the implementation of unlinked HIV screening programs. Clear legal authorization for unlinked anonymous HIV testing is provided for by statute or regulation in the U.S. Federal regulations and various state laws specifically permit unlinked anonymous testing. Under these regulations and statutes, informed consent of a test subject is not required so long as testing is done for research purposes and the test results are not individually traceable to particular subjects.

AIDS Serodiagnosis↗
PubMedDate not supplied

Serum estradiol-binding profiles in postmenopausal women undergoing three common estrogen replacement therapies: associations with sex hormone-binding globulin, estradiol, and estrone levels.

OBJECTIVE: To compare the effects of three commonly prescribed estrogen replacement therapies-oral conjugated equine estrogens (CEE; n = 37), oral micronized estradiol (ME; n = 25), and transdermal estradiol (TE; n = 24)-on the binding characteristics of plasma estradiol as related to the concentrations of blood sex hormone-binding globulin (SHBG), estradiol, and estrone. DESIGN: Menopausal volunteers, opting for estrogen replacement therapy, gave blood at 0, 2, and 4 months. SHBG was assayed by automated immunoabsorbent technology. Estradiol and estrone were determined by quantitative gas chromatography/mass spectrometry. After tritiated estradiol was added to serum, the percentage of estradiol not bound to protein was determined by ultrafiltration and the percentage of estradiol bound to SHBG was measured by a method exploiting that this protein, even when bound to estradiol, binds avidly to Concanavalin A-Agarose. RESULTS: In each study, 2- and 4-month data were similar. Increases in SHBG concentrations were 100% (p < 0.001), 45% (p < 0.001), and 12% (nonsignificant) for subjects who were receiving CEE, ME, and TE regimens, respectively. Decreases in the percentage of estradiol not bound to protein and increases in the percentage of estradiol bound to SHBG correlated with changes in the concentrations of this protein mediated by the therapies. The order for increases in estradiol was ME-TE >> CEE, whereas for estrone, the order was ME > CEE >> TE, divergent from the SHBG responses. CONCLUSIONS: The diverse responses observed can be explained by differences in the estrogen load delivered to target tissues as controlled by the intermediary circulation and metabolism of the hormones introduced in these regimens.

Adult↗
PubMedDate not supplied

Heterogeneous glycosylation of cationic peanut peroxidase.

Cationic peanut peroxidase (CPrx) from a cell suspension culture is N-glycosylated at Asn60, Asn144, and Asn185. All three N-glycans are complex type and galactose rich, and show heterogeneity in length and ConA (concanavalin A) binding property. The glycan heterogeneity causes a polymorphism of the enzyme. Based on its behavior on ConA columns, CPrx can be grouped into two fractions: nonbinding (CPrx-) and binding (CPrx+) types. A synchronously cosecreted beta-galactosidase has been discovered in the culture medium; there are two isozymes of 60 kDa (pI 7.3) and 66 kDa (pI 7.6). This beta-galactosidase has been partially purified by a combination of ion-exchange and size-exclusion chromatographies and preparative isoelectrofocusing. In vitro experiments indicate that the cosecreted beta-galactosidase is able to convert peroxidase from CPrx- to CPrx+ and may, to some extent, contribute to the glycan heterogeneity of peroxidase in the cell culture.

Arachis↗
PubMedDate not supplied

[Long-term intermittent renal replacement therapy at an intensive care unit].

Standard intermittent hemodialysis (IHD) used for the treatment of acute renal failure (ARF) at an intensive care unit has significant biochemical and physiological drawbacks. In the past 20 years, these drawbacks have stimulated the development of continuous renal replacement therapy (CRRT) and its ever-increasing use. However, CRRT is technically complicated and requires 24-hour monitoring. In some clinics, the use of CRRT leads to that each patient is under his/her nurse's surveillance, instead 1 nurse per 2 patients as before; this change has economic consequences and may limit nursing accessibility to other patients. The procedures prolonging intermittent therapy do not require 24-hour monitoring may benefit the treatment of ARF at the intensive care therapy. In this paper the authors call such procedures for continuous intermittent renal replacement therapy. They are characterized by a number of basic principles: (1) the use of modified or standard dialysis apparatuses; (2) the application of diffuse, convection, or both; (3) a certain reduction in the rate of elimination of dissolved substances as compared with IHD; (4) more prolonged treatment: above usual 3 or 4 hours of IHD, but not more than 8-12 hours (hence the term "intermittent"); (5) the use of on-line generation dialysate or substituting fluid. Information on the effectiveness and safety of this procedure is being now compiled.

Acute Kidney Injury↗