[Clinical application of mifepristone in obstetrics and gynecology].
Explore the source record for details and available documents.
Abortifacient Agents, Steroidal↗
Biomedical subjects
L Weng
Publications and source records attributed to L Weng.
[National symposium on clinical application of mifepristone in obstetrics and gynecology].
Explore the source record for details and available documents.
Abortifacient Agents, Steroidal↗
[Clinical experience with iron supplementation in pregnancy].
OBJECTIVE: To study the efficacy of iron supplementation during pregnancy and its influences on the outcome of pregnancy. METHODS: A total of 369 pregnant women were enrolled in this study. According to the hemoglbin levels at recruitment, there were 2 groups: preventive (Hb > or = 110 g/L) and treatment (Hb < 110 g/L) groups. In the preventive group, women entered the study from 20-24 gestational weeks and were randomly assigned to materna treatment (n = 96) who took materna 1 tablet daily or control group (n = 95) who took no other supplementation. In the treatment groups, women less than 36 gestational weeks were accepted. They were randomly divided as materna (n = 93) 1 tablet/d, ferrous sulfate 0.3 g tid/d (n = 50) or Ferroids 1 tablet/d (n = 35) groups. Both Hb and serum ferritin concentrations were determined at admission and immediately after delivery. In some cases serum ferritin in the umbilical vein were measured as well. Hemoglobin levels were examined every 4 weeks during the observational period. RESULTS: In the preventive groups, maternal serum ferritin levels after materna treatment were significantly higher than that before treatment and the control group (P < 0.05). As for the anemia women, compared the serum ferritin concentration, materna treatment had significantly higher levels than that at admission (P < 0.05), and also higher than that in the ferrous sulfate or ferroids tratment groups (P < 0.05). The s-ferritin in the umbilical vein had no correlation with the paired maternal levels. There were no significant differences in the pregnancy outcomes among all the groups. CONCLUSION: Materna can increase the iron storage and effectively improve the iron deficiency during pregnancy, and has no impact on the prgnancy outcomes.
Adult↗
[Effects of the new compound-XW630 on osteoblast].
This study aimed to observe the influence of the new compound-XW630 on the proliferation of osteoblast, ALP action, and the forming of osteoclast. The MTT method and the alkaline phosphatase method were adopted to investigate the influence of XW630 on ALP action and the proliferation of osteoblast cultured from grown rat's skull. The Tartrate-resistant acid phosphatase dyeing method was used to observe the influence of XW630 on forming of cultured osteoclast in vitro. The result showed that, when XW630 concentration was 10(-6)-10(-8) mol/L, it obviously enhanced the proliferation of osteoblast and improved the activity of ALP, and it evidently provented PTH from stimulating the forming of osteoclast. It is concluded that XW630 is obviously effective for stimulating the proliferation of cultivated osteoblast in vitro and for inhibiting the forming of osteoclast.
Alkaline Phosphatase↗
[The estrogenic activities of 2-[3-estrone-N-ethyl-piperazine-methyl]tetracycline(XW630)--a new compound with anti-osteoporosis activity].
2-[3-Estrone-N-ethyl-piperazine-methyl] tetracycline (XW630) showing anti-osteoporosis activity is a new compound with piperazine-tetracycline in the steroid structure of estrone. The affinity of XW630 with uterine estrogen receptor and its estrogenic activity were studied in this paper. The relative affinities of estrone, estradiol and XW630 with estrogen receptor (ER) of the uterine myoma tissues were found to be 0.325, 1.000 and 0.011, respectively, by the competitive assay. The estrogenic activity index of estrone, estradiol and XW630 were shown to be 9.70, 10.30 and 6.90, respectively, by the uterine weight assay using immature mice. These indexes indicate that: the estrogenic activity of XW630 is about 1/1960 that of estrone and 1/7742 that of estradiol. The vaginal smear assay of oophorectomized mice also showed that the estrogenic activity of XW630 is less than that of estrone and estradiol.
Animals↗
[Synthesis and antiosteoporosis activity of steroid-bisphosphonate compounds].
Bisphosphonate have bone-seeking affinity, some of them are used as drugs for osteoporosis. Steroidal hormones are often used in prevention and treatment of osteoporosis. In order to search for bone-targeted leading compounds with high affinity and low toxicity, seven steroid-bisphosphonate compounds were synthesized. These compounds were found to promote bone formation in bone cell culture.
Animals↗
[Bio-mechanical property changes of long bone in ovariectomized goats].
This paper presents the biomechanical property changes of long bone in goats at 180 days after ovariectomy. 10 female goats at the age of 1.5 +/- years(average body wight 22 kg) were randomly divided into the control group and ovariectomized group. These goats were sacrificed at 180 days after ovariectomy and the femora, tibiae, metatarsus, humeri, radius and metacarpus were taken out bilaterally. The structural mechanical properties of these bones were measured with the three-point bending method. The material mechanical properties of flexura, compress and tensile in the compact bone specimens taken from the two broken ends were also measured. The results showed that the bone density, bone thickness in broken ends, and the break load of three-point bending for the long bones in the OVX group were obviously lower than those in the control group (P < 0.05). And compared with the control group all the long bones in the OVX group, except the femur, had significantly lower ultimate bending strength, bending elastic modulus; ultimate flexural strength, flexural elastic modulus; ultimate compressing strength, and compressing elastic moduus. Some long bones in the OVX group had significantly decreased ultimate tensile strength (P < 0.05). These indicated that the mechanical properties of long bone in OVX group at 180 days after ovariectomy were reduced in varying degrees and were liable to fracture. This result is in accord with the clinical findings in osteoporosis resulting from post-menopausal estrogen fall and is also consistent with the osteoporotic model of ovariectomized rats. The authors conclude that the bone biomechanical properties are important parameters in assessing osteoporosis and the ovariectomized goat can possible be a good big animal model of osteoporosis.
Animals↗
[A biomechanical evaluation of the effects of XW630 on the healing of experimental fracture].
The aim of this study was to evaluate whether tetracycline-estrone (XW630) plays a role in the healing of fracture. 120 rabbits were divided into 4 groups and subjected to osteotomies at the middle of both radials, and they were given XW630, estrone (E2), tetracycline (T) and 0.9% sodium chloride (C) respectively after operation by means of intramuscular injection (M) or local injection(L) in the fracture region. The biomechanical properties, including bending and compression, and the biochemical changes were tested at 30 days, 60 days and 90 days after osteotomy. The results showed that the bending stress and compression stress in the XW630(M) group were significantly higher than those in other groups at different periods after osteotomy. At 30 days after osteotomy, the bending ultimate stress in the XW630(M) group was 80% higher than that in group C, 76% higher than that in group E2, and 69% higher than that in group T. At 90 days after osteotomy, the bending ultimate stress in XW630(M) group increased to 80% of that of the normal radius, and it was 30% higher than that of group C, 34% higher than that of group E2 and 19.5% higher than that of group T. The biochemical changes corresponded with the changes biomechanical properties. These data suggest that XW630 given by intramuscular injection plays an obvious role in accelerating the healing of fracture. The medicine given by intramuscular injection is obviously more effective than that locally injected.
Animals↗
US extended-field-of-view imaging technology.
PURPOSE: To develop an ultrasound (US) extended-field-of-view scanning technique that combines the convenience of a real-time scanner with the spatial advantages of a static B-mode scanner and provides a panoramic image in real time without position sensors or cumbersome articulated arms. MATERIALS AND METHODS: An image-registration-based position-sensing technique was used to track probe motion and reconstruct a large composite image during real-time scanning. The probe motion (translation and rotation) was estimated by combining multiple local motion vectors. This computationally intensive process required a special programmable image processor. RESULTS: Large, resolution-preserved composite images up to 60 cm long were obtained. Measurement accuracy as determined with phantom experiments was better than 5%. The method could be applied to any probe or image format. CONCLUSION: In addition to providing a panoramic image to expand diagnostic capabilities, extended-field-of-view US provides a more easily interpretable image and is an effective cross-specialty communication tool.
Abscess↗
[Bone histomorphometric changes in ovariectomized goat at different time courses].
Using histopatholoical method, we investigated the bone histomorphometric changes in ovariectomied goats at different time courses. Eight goats, female, aged one and a half years, were randomly divided into two groups, the control and the ovariectomy (OVX). Iliac crest biopsies were separately processed before ovariectomy and at 60 days, 120 days and 180 days after ovariectomy. The histomorphometric changes were observed. It was found that the bone structure in trabecular bone remained relatively constant in the control group throughout the whole study. In contrast, the trabecular quantity was slightly decreased and the trabeculae were disconnected in OVX at 60 days after ovariectomy. The bone structure was characterized by the thinning of the trabeculae, the further discontinuance and quantity loss of trabeculae, and the anlargement of marrow cavity at 120 days to 180 days after ovariectomy. Apparently, these bone structural changes manifested with typical osteopenia pathological changes. Our results may serve as a basis for the design of further studies using OVX goat as an animal model for postmenopausal osteoporosis.
Animals↗
[Cloning and expression in Escherichia coli of an alkaline and thermostable exolipase from Pseudomonas pseudoalcaligenes].
A gene coding for an alkaline and thermostable exolipase of Pseudomonas pseudoalcaligenes was cloned into Escherichia coli LE392 by inserting Sau3 AI-generated DNA fragments into the BamHI site of pIJ285. Four colonies with esterase and lipase activities on the tributyrin agar plate were isolated by screening the constructed pseudomonas pseudoalcaligenes genomic library. Only one out of the four positive colonies showed lipase activity on the agar plate contatining olive oil and Rhodamine-B. Subclones of the 45 kb insert carrying lipase gene was obtained in E. coli HB101 using pUC118 as a vector, two of which (HB101 (pHZ1402) and HB101 (pHZ1403)), retained lipase activity, but the level seems to be different. They carried 3.0 kb and 2.9 kb inserts with a 2 kb overlapping sequence. The lipase activity of HB101 (pHZ1403) is about 4 times higher than that of HB101 (pHZ1402), as 5 times as that of original strain P. pseudoalcaligenes.
English Abstract↗
[Primary study on the use of ovariectomized rabbits for establishment of osteoporosis model].
Purebred New Zealand female rabbits were used in this study for demonstrating the feasibility of establishing rabbit's primary osteoporosis model. Bilateral ovariectomies were performed. Five to eight months after operation, we observed the BMD, weight, hair colour and living condition of the model. After killing the rabbits, we made biomechanical detection and pathologic histomorphometry using the bones. The weight of bone ash, the content of calcium and phosphorus, and the weight of metra were determined. The results showed that BMD, bone bending stress, weight of bone ash, content of calcium and phosphorus in ash, and weight of metra all decreased (P < 0.05); the number of trabecula decreased and the bone cortex became thinner. All these parameters evidenced the primary founding of rabbit's osteoporosis model. This research provides a basis for studying the pathogenesis of primary osteoporosis and evaluating the efficacy of pharmacotherapy.
Animals↗
[Clinical analysis of bacterial vaginosis in 76 cases].
OBJECTIVE: To study the clinical manifestation and treatment of bacterial vaginosis (BV). METHODS: The criteria used for diagnosis were the presence of clue cells by wet mount slide or gram stain, plus at least 2 of the following 3 signs and symptoms: (1) vaginal discharge with pH > 4.5; (2) an increased thin homogeneous vaginal discharge; (3) positive amine test. From 1994, 1 to 1995, 3, a total of 76 cases with BV who had good follow up were diagnosed and treated by Kejunning (a combination of chinese traditional herb medicine), Shuangzuotai (combined metronidazole vaginal suppository), metronidazole, etc. RESULTS: For BV, the presence of clue cells is a prerequisite for diagnosis. Vaginal discharge with pH > 4.5 and positive amine test were present in all the cases. Increased white thin homogeneous vaginal fluid were found in 96.1% (73/76) of the cases. 79.0% of the patients complained of fishy odor vaginal discharge. Treatment with antibiotics or Shuangzuotai were effective. Kejunning had a cure rate of 70.0% one month after treatment. CONCLUSIONS: The detection of clue cell is an important diagnostic criterion. Besides metronidazole, Kejunning had a good result for the treatment of BV as well.
Adult↗
[Contraceptive research and development].
Explore the source record for details and available documents.
Contraceptives, Oral, Synthetic↗
[Emergency contraception with mifepristone and anordrin].
OBJECTIVE: To study the efficacy of mifepristone or with anordrin for emergency contraception. METHODS: 300 healthy women were recruited within 7.2 hours after unprotected intercourse or contraceptive failure and randomly allocated into 3 groups. Group 1 (n = 100), mifepristone 25 mg twice with 12 hours apart; group 2 (n = 99), single dose of mifepristone 25 mg; and group 3 (n = 101), mifepristone 25 mg and anordrine 7.5 mg given once. RESULTS: No pregnancy occurred in group 1, while 1 pregnancy in each group 2 and 3. The contraceptive effectiveness were 100.0%, 83.8% and 86.1% for the 3 groups respectively. The overall menstruation disturbances and side effects were low. CONCLUSIONS: Both mifepristone 50 or 25 mg were effective for emergency contraception and no synergetic effects of anordrin in combination with mifepristone was shown in this study.
Adult↗
[Mifepristone for induction of labor].
OBJECTIVES: To evaluate the effectiveness of mifepristone administered prior to labor induction, and to study its safety for mother and fetus. METHODS: 124 nullipara, 37-42 gestational weeks, with indications for labor induction were recruited, and randomly allocated into 2 groups. Group A (n = 62) was given mifepristone 50 mg q.12.h. for 2 days, followed by PG05 or oxytocin, while group B (n = 62) was were observed for 2 days before labor induction by PG05 or oxytocin. Blood samples were obtained for determination of mifepristone concentration and hormone levels including estradiol, progesterone, testosterone, cortisol, aldosterone and human placental prolactin at recruitment and immediately after delivery, and umbilical cord blood was collected at the same time. RESULTS: Cervical Bishop score increased significantly in the mifepristone pretreatment group when compared with the control group. 22.58% of the women underwent spontaneous delivery after mifepristone treatment and 4.84% of the controls followed suit (P < 0.01). The oxytocin dose required was significantly less in group A, but the success rate was higher (P < 0.05). Side-effects associated with mifepristone was mild. Maternal serum mifepristone peak levels ranged from 200 to 700 micrograms/L, with t1/2 of 21.7 hr. The concentration of mifepristone in umbilical blood was low and stable. The ratio of umbilical/maternal mifepristone level was 0.25 +/- 0.08. Determination of hormone profiles did not show any significant difference between the 2 groups. CONCLUSIONS: Mifepristone is an effective inductive agent for cervical ripening and initiation of labor in term pregnancy, and can improve the outcome of labor induction.
Abortifacient Agents, Nonsteroidal↗
[Synthesis of polyfunctional bridging agent-tetrakis (omega-hydroxyproxymethyl) methane and tri (hydroxyproxymethyl) amino methane].
In order to look for the polyfunctional bridging agent. Tetrakis (omega-hydroxyproxymethyl) methane (3) and Tri (omega-hydroxyproxymethyl) aminomethane (4) were prepared from pentaerythritol and trihydroxymethylamino methane respectly. Nine derivatives (5-13) of compounds (3) and (4) were synthesized. The structures of all new compounds (5-13, 15) were confirmed by IR, 1H-NMR and elemental analysis.
Bridged-Ring Compounds↗
[Influence of dihydroetorphine hydrochloride and tramadol on labor pain and umbilical blood gas].
Ninety primigravide were randomly allocated into three groups at the beginning of active phase of labor. Dihydroetorphine hydrochloride (DHE) was administered to group A (n = 30), tramadol to group B (n = 30), and group C (n = 30) was a blank control. Various parameters about analgesia effects, progress of labour and fetal and neonatal well-being were investigated and also umbilical artery and vein blood gases analyzing were carried out in all cases. As a result, the effective rate of pain relief in group A was 67% and in group B 63% (P > 0.05). The average time of onset of action in DHE group was 16.5 +/- 2.9 minutes which was significantly shorter than 26.1 +/- 5.4 minutes in tramadol group (P = 0.0001). There were higher rate (36.7%) of operative intervention (forceps and vaginally or cesarean section) and a higher average amount of postpartum hemorrhage in group A, as compared with the control group, but no significant difference was shown between group B and group C. No difference was found in other parameters (among the three groups duration of labour, Apgar scores of neonates, cord blood gases, etc.). The conclusion is that, both DHE and tramadol may have a good effect of pain relief in labour. The time of onset of action in DHE group is significantly shorter than that in tramadol group. Neither analgesics cause circulation and respiratory depression in the mother and neonates, but DHE may have influence on uterine contraction. To achieve an excellent pain relief by higher dosage of DHE and tramadol need further randomized investigation.
Analgesia, Obstetrical↗