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Biomedical subjects

L Woźniak

Publications and source records attributed to L Woźniak.

At least 19 recordsLinked to original sources
PubMed1996

Phenotypic heterogeneity of nodular hidradenoma. Immunohistochemical analysis with emphasis on cytokeratin expression.

We studied the immunohistochemical phenotype in 13 cases of the nodular hidradenoma (NH), with special emphasis on the expression of different types of keratins (cytokeratins, 7, 10, 6/18, 8/18, and 10/17/18 and their distribution in normal sweat glands. Variable reactions with keratins, alpha-smooth muscle actin, and epithelial membrane antigen (EMA) were found, as these markers were present in different cellular components of the tumors. The most constant finding was almost complete absence of cytokeratins (all but keratin 10/17/18, which was positive in two of 13 cases) in clear cells, which yet were positive for EMA. The tumors expressed mostly cytokeratin 6/18, 7, 8/18, and 10/17/18, which were found in 11, 13, 11, and 12 cases, respectively. The cellular distribution and quantity of stained cells differed, as keratins 6/18, 8/18, and 7 produced the most abundant staining and were predominantly localized in small squamoid cells and the cells lining the tubular and cystic spaces. Cytokeratin 10/17/18 was expressed in smaller or larger clusters of squamoid cells and rarely in clear cells. Cytokeratins 10, 19, and 20 were found sporadically in single cells or small cellular clusters. alpha-Smooth muscle actin was expressed in four cases, whereas we did not find reactivity of S-100 protein. Comparing these results with the pattern of keratin distribution and antigenic reactivity in eccrine sweat glands, we conclude that NH presents cellular heterogeneity of its elements and differentiation toward different parts of the sweat gland.

Actins↗
PubMed1996

Carcinosarcoma of the skin. Case report and literature review.

A case of carcinosarcoma arising in the skin of the left arm of a 69-year-old woman is reported with a review of the literature. The tumor was composed of low-differentiated squamous cell carcinoma, which was intermingled with a pleomorphic sarcoma. The carcinomatous component had keratin and lacked vimentin, whereas the phenotype of the sarcomatous portion was the reverse. The former presented additionally focal expression of S-100 protein, which was lacking in other portions of the carcinoma. The phenotypic data, supplemented by p53 immunostaining, which was present in both components, suggest their common origin in this tumor.

Aged↗
PubMed1996

Expression of the latent membrane protein of Epstein-Barr virus in Hodgkin's disease. Age and subtype distribution in Polish patients.

We examined immunohistochemically 135 cases of HD (4, lymphocytic predominance (LP); 34, mixed cellularity (MC); 90, nodular sclerosis (NS) and 7, lymphocytic depletion (LD) for the presence of EBV latent membrane protein (LMP). Ten patients were younger than 14 years, 55 were young adults (15-34 years), 45 were 35-49-years old and 26 were older than 50 years. The average LMP-immunopositivity was 33% of all HD cases. The highest proportion of LMP-immunopositivity was found in the MC subtype (61.8%), followed by the NS (NS 1-25%, NS 2-26%) and the LD subtype (14%), but none in the LP subtype of HD. The differences between LMP-immunopositivity in the MC and NS subtypes were statistically significant in children and young adults (p < 0,01). The LMP-positivity was mainly associated with the MC subtype which occurred predominantly in children, while the lowest proportion of LMP-positive cases was found in the young adult group. This report supports a notion that there are regularities in age and subtype distribution of EBV in HD.

Adolescent↗
PubMed1996

p53 expression in sweat gland tumors.

We analyzed the expression of p53 in 74 cutaneous adnexal tumors, with enhancement of the detection by incubation of the slides in the microwave. The immunostaining in benign tumors was almost uniformly negative as we found p53-positivity only in one poroma, one nodular hidradenoma, and one case of syringocystadenoma papilliferum (amongst 13 spiradenomas, 9 cylindromas, 12 nodular hidradenomas, 7 poromas, 6 syringomas, 7 syringocystadenomas papilliferum, 2 papillary tubular adenomas and 4 chondroid syringomas). These results contrasted with the widespread p53 overexpression, which was revealed in the sweat gland carcinomas. All spiradenocarcinomas (3), malignant nodular hidradenoma (1), apocrine hidradenocarcinoma (1), and malignant syringoadenoma (1) showed a strong reaction to anti-p53 antibody. Two of three eccrine hidradenocarcinomas, and two of three porocarcinomas presented p53 overexpression, whereas in one case of malignant cylindroma and adenoid cystic carcinoma we did not find p53-positivity. The results of the study indicate an important role, that p53 protein plays in the malignant sweat gland tumors in comparison to their benign counterparts, but reveal that its overexpression may also occur in the reactive and benign neoplastic processes.

Gene Expression Regulation, Neoplastic↗
PubMed1996

Diagnostic significance of the myoepithelial cells in the benign and malignant sweat gland tumors.

We studied the presence of myoepithelial cells (MCs) in the benign and malignant sweat gland adenomas by immunostaining with antibody to alpha-smooth muscle actin. We found peripheral arrangement of MCs in the neoplastic nests in cutaneous cylindromas (9), spiradenomas (13), syringoadenomas (7), and one case of adenoid cystic carcinoma. Dispersed MCs were identified in some cases of nodular hidradenoma (7/13) and malignant portion of spiradenocarcinoma (2/3). The remnants of the peripheral arrangement of MCs were retained in the benign part in cylindrocarcinoma and every case of spiradenocarcinoma, likewise in composed malignant adnexoma (malignisation within cylindroma/spiradenoma). We could not find MCs in papillary eccrine adenoma (2), poromas (7) and porocarcinomas (3), syringomas (6), chondroid syringomas (3), malignant nodular hidradenoma (1) and malignant portion of syringoadenoma (1). In one of three cases of eccrine hidradenocarcinoma focal myoepithelial differentiation could be identified at the periphery of the epithelial nests. These results confirm heterogeneity of the differentiation in sweat gland tumors, emphasizing the validity of division of those tumors into those with the differentiation towards secretory and ductal portion of the gland. The former group demonstrates the variability of arrangement of myoepithelial cells depending on the degree of dedifferentiation of the developing carcinoma. These cells may be replaced by outgrowing tumor, but may also accompany the neoplastic growth. Less significant is the presence of the MCs in the adenomas with ductal differentiation, which either in benign and malignant tumors may be lacking. The variability of MCs occurrence in cutaneous adenomas and carcinomas precludes its significance as a solitary factor in the differentiation between benign and malignant proliferations.

Diagnosis, Differential↗
PubMed1995

Over-expression of p53 protein as an indicator of the malignant transformation in spiradenoma.

Malignant spiradenomas (spiradenocarcinomas) are exceedingly rare tumours of cutaneous adnexal origin, consisting of two components: benign--the pre-existent adenoma, and malignant--developing from the former part. We studied p53 protein expression in both compartments of three cases of malignant spiradenoma and compared these results with results obtained with eight cases of spiradenoma. Nuclear staining was consistently negative in all benign tumours, whilst in the cases of malignant transformation within spiradenoma p53 protein was present in the carcinomatous component, but the immunostaining remained negative in the benign counterpart of the tumour. In the zone of transition between both components of the spiradenocarcinomas p53 expression was positive in the cells with morphological atypia, providing clear discrimination. Thus, we conclude that the accumulation of p53 protein, which results from alterations in its turnover, accompanies the process of malignant transformation within long-standing spiradenomas.

Adenoma, Sweat Gland↗
PubMed1995

Detection of EBV mRNA in nodular sclerosis type of Hodgkin's disease using in situ hybridization with EBER 1 and EBER 2 probes with regard to the immunophenotype of Reed-Sternberg cells.

Hodgkin's disease (HD) is a heterogenous disorder in terms of morphology, immunophenotype, genotype and association with viruses. Seroepidemiologic, immunohistochemical and molecular studies suggest that Epstein-Barr Virus (EBV) plays an important role in the pathogenesis of HD. We used in situ hybridization (ISH) with digoxigenin-labeled EBER 1 and EBER2 probes to examine a series of 28 lymph node specimens from untreated patients from Poland with the nodular sclerosis NS 1 and NS 2 subtypes of HD to detect EBV mRNA in Reed-Sternberg cells (RSC), or in small non-neoplastic lymphocytes called "bystander cell" (ByC). EBV mRNA was detected in 12 of 28 cases (32.1%). Furthermore, the expression of Latent Membrane Protein (LMP) of EBV strongly correlated with expression of EBER 1 and EBER 2 in RSC but not in "bystander cells", which were LMP negative. The correlation between EBV status and immunophenotype of RSC are discussed.

Herpesvirus 4, Human↗
PubMed1995

Cellular blue nevi with myxoid change--diagnostic difficulties and the review of the literature.

We report three cases of a rare morphologic variant of the cellular blue nevus, which usually occurs in young patients on the buttocks and lumbar area. This variant is characterized by the myxoid degeneration of the stroma, which separates alveolar nests of nevus cells. The uncommon occurrence and atypical pathologic features may bring about diagnostic pitfalls and unnecessary excessive treatment modalities, therefore, we tried to re-evaluate the criteria of diagnosis of cellular and malignant blue nevi.

Adolescent↗
PubMed1995

Malignant blue nevus with neurosarcoma-like lymph node metastases.

We report a case of malignant blue nevus (MBN) of the foot with unusual morphologic presentation of nodal metastases. Their structure resembled neurosarcoma, and differed from the appearance of the primary tumor. This case confirms the mutual close histogenic relationship between MBN and neurosarcoma, both tumors originating from the structures of the neural crest. We also discuss the problem of so-called "benign" metastases of malignant melanoma.

Biopsy↗
PubMed1994

Expression of bcl-2 protein and Epstein-Barr virus latent membrane protein in Hodgkin's disease.

The presence of Epstein-Barr virus (EBV) correlates with some cases of Hodgkin's disease (HD), and its latent membrane protein (LMP) has oncogenic potential by inducing expression of bcl-2 protein. Bcl-2 confers a longer half-life to the cell, which overexpresses it. As the translocation t(14,18), which is most often found in follicular lymphomas, and leads to overexpression of bcl-2, has also been reported in HD, it is possible that there is a correlation between these events in this entity. We stained immunohistochemically 40 cases of HD for the presence of bcl-2 and EBV-LMP. Bcl-2 positivity within reactive lymphocytes was revealed in 29 cases. In five of these cases a week, positive reaction in cytoplasm of Reed-Sternberg cells was observed (one mixed cellularity and four nodular sclerosis cases). The EBV-LMP immunopositivity was observed in 16 of these 29 cases (ten MC and six NS cases). The simultaneous presence of bcl-2 protein and EBV-LMP was found in two cases, the remaining three bcl-2-positive cases did not have EBV-LMP. These results do not support the hypothesis of the correlation between the expression of bcl-2 protein and the presence of EBV-LMP in the pathogenesis of HD as the LMP-dependent stimulation of bcl-2 oncogene.

Antigens, Viral↗
PubMed1994

Papillary eccrine adenoma--a case of cutaneous sweat gland tumor with secretory and ductular differentiation.

We report a case of the papillary tubular adenoma (PTA) with histological features of differentiation toward secretory and ductal portions of the eccrine gland (papillary eccrine adenoma). The papillary eccrine adenoma (PEA) is a rare benign adnexal neoplasm of the skin characterised by confined growth; however, in some cases it may present a locally aggressive behaviour. This tumor has a unique histopathological appearance, but sometimes the differential diagnosis with other malignant adnexal neoplasms may be difficult and become a source of excessive surgical procedures. We emphasized the need of the assessment of the whole arrangement of the tumor (symmetry, perpendicularity of its long axis to the epidermis, its own fibrous stroma well separated from the surrounding dermis), which prevents the diagnostic pitfalls with adnexal cutaneous carcinomas.

Adenoma, Sweat Gland↗
PubMed1993

Percentage of proliferating cell nuclear antigen (PCNA)-positive Reed-Sternberg cells in nodular sclerosis Hodgkin's disease.

Proliferating cell nuclear antigen (PCNA/cyclin) is a useful marker of proliferation and its expression correlates with prognosis in some human neoplasms. The clinical course of nodular sclerosis Hodgkin's disease (NSHD) varies in both grades (NSI, NSII) as defined by the British National Lymphoma Investigation. Expression of PCNA in Reed-Sternberg cells and their variants was evaluated in NSI and NSII. There were no significant differences in the ratio of PCNA-positive cells in both groups. The results suggest that differences in the clinical course do not depend on the proliferative rate of Reed-Sternberg cells and their variants in NSHD.

Antigens, Neoplasm↗
PubMed1993

[Comparison of computed tomography results and pathological findings after laparotomy with splenectomy in Hodgkin's disease].

In 69 patients (pts) with Hodgkin's disease (HD) in I-IIIA clinical stage results of abdominopelvic computed tomography (CT) were compared with pathological staging. Concordance (specificity) of CT scan with results of macroscopic evaluation of HD lesions in the abdomen during laparotomy with splenectomy was 83%. Accuracy CT results and microscopic findings was 80%. False positive results of CT were found in 11.6% of patients and false negative in 8.6%. Specificity and sensitivity of CT versus to pathological staging were 84% and 63% respectively. The results presented here confirm, that pathological staging after laparotomy with splenectomy in HD was some advantage in precise prediction of some patients to radiotherapy and planning of irradiated fields.

Adolescent↗