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Biomedical subjects

L Wozniak

Publications and source records attributed to L Wozniak.

14 recordsLinked to original sources
PubMed1998

p53 mutations in sweat gland carcinomas.

Sweat gland carcinomas are rare skin tumours and little is known about their etiology and molecular basis. In this study, we analyzed p53 mutations in 16 sweat gland carcinomas with different histologic types, including 2 spiradenocarcinomas, 1 composed adnexal carcinoma, 5 porocarcinomas, 2 eccrine hidradenocarcinomas, 2 syringocystadenocarcinomas, 1 sclerosing sweat gland carcinoma, 1 adenoid cystic carcinoma, 1 cylindrocarcinoma and 1 apocrine adenocarcinoma. Single-stranded conformation polymorphism (SSCP) analyses followed by direct DNA sequencing revealed that 5 carcinomas (31%) contained a p53 mutation, 4 of which were G:C-->A:T transition mutations and 1 of which was a deletion. Three G:C-->A:T mutations were located at dipyrimidine sequences on the antisense strand (2 spiradenocarcinomas, 1 eccrine hidradenocarcinoma), suggesting that UV light may play a role in the development of sweat gland carcinomas. In 2 spiradenocarcinomas, p53 mutations were present in the carcinoma but not in the adenoma portions, suggesting that p53 mutations may be associated with malignant progression in these rare adnexal tumours.

Adenocarcinoma↗
PubMed1994

Spiradenocarcinoma: a clinicopathologic and immunohistochemical study of three cases.

Three cases of malignant transformation within spiradenoma are described. All of them occurred close to the elbow. In every case we found a benign component, characteristic of the spiradenoma, and a structure of carcinoma, which in some areas showed transition into the spiradenoma. In one case the structure of the spiradenocarcinoma resembled that of the spiradenoma, whereas in the remaining two cases adenocarcinoma was the malignant counterpart of the tumor. We performed immunohistochemical studies and revealed various intensities of expression of cytokeratins and epithelial membrane antigen and a dearth of S-100 protein, vimentin, and carcinoembryonic antigen in the examined cases of spiradenocarcinoma.

Adenocarcinoma↗
PubMed1989

Neuroendocrine (Merkel cell) carcinoma of the skin: a clinico-morphological study of 13 cases.

The clinico-morphological features in 13 patients (nine female) with neuroendocrine carcinoma of the skin are presented. The mean age was 64.9 years. The limbs were the most common site of primary tumour, followed by the face. The clinical course was characterized by a high incidence of regional lymph node metastases (69%) and recurrences (46%). Seven of the patients died of tumour, with a mean survival time of 13 months. Histologically, a solid pattern of tumour growth was most common. The cells were usually small and uniform. Squamous cell differentiation was found in one tumour. The cell of all tumours reacted positively for cytokeratins and neuron-specific enolase. The positive reaction frequently had a ball-like globular pattern, corresponding to inclusion-like bodies seen on light microscopy and to paranuclear whorls of intermediate filaments observed on electron microscopy. Neurosecretory granules were seen on electron microscopy in the 11 cases examined and in one case a 'Luse body' was found in the intercellular space.

Aged↗
PubMed1984

Biosynthesis of the glycocinnamoylspermidine antibiotic, cinodine.

The biosynthesis of cinodine from a combination of 14C- and 13C-labeled precursors has been investigated. Tyrosine was shown to be incorporated efficiently into the cinnamoyl moiety and glucosamine was found to be the origin of the three carbohydrate moieties. The relationship between the substrate dose and the enrichment of the labeled antibiotic has been elucidated so that it is possible to predict both the specific activity and the yield of the antibiotic obtained from the labeled substrates.

Aminoglycosides↗
PubMed1981

Pseudodendritic keratitis and systemic tyrosinemia.

Three children (6 eyes) with systemic tyrosinemia who presented with a diagnosis of herpes simplex keratitis are described. Two of the patients underwent extensive treatment for herpes simplex keratitis until their defects in tyrosine metabolism were discovered. All three children responded favorably to a low-tyrosine diet. These cases illustrate the typical corneal findings of the Richner-Hanhart syndrome (pseudodendritic keratitis, hyperkeratotic skin lesions of the palms and sole without mental retardation). The findings in these three patients were similar to those cases previously reported in the literature except none had mental retardation. The ophthalmologist confronted with a young child with bilateral dendritiform keratitis should request evaluation of the serum tyrosine levels.

Amino Acid Metabolism, Inborn Errors↗
PubMedDate not supplied

Neuroblastic differentiation of metastases of medulloblastoma to extracranial lymph node: an ultrastructural study.

Extracranial metastases of a poorly differentiated medulloblastoma in a 12-year-old girl were studied by thin section transmission electron microscopy and immunohistochemistry. The primary tumor did not show any differentiation as revealed by immunohistochemistry. On the contrary, the metastatic tumor cells and their processes disclosed features of neuroblastic differentiation when examined ultrastructurally: microtubules, dense core vesicles, and abortive synaptic ribbons. Several dystrophic neurites containing altered subcellular organelles were also found. Furthermore, few processes contained concentric arrays of paired membranes. This report is the first to clearly show the neuronal differentiation of extracranial metastases of poorly differentiated medulloblastoma. We speculate that metastases of medulloblastomas outside the neuraxis behave analogously to medulloblastoma explants cultured in vivo.

Antineoplastic Combined Chemotherapy Protocols↗
PubMedDate not supplied

Decisive role of intermediate filament typing of tumor cells in the differential diagnosis of difficult fine needle aspirates.

Thirty-six diagnostically difficult fine needle aspirates from enlarged lymph nodes and malignant soft tissue tumors, containing tumor cells with scanty or no obvious light microscopic features indicative of their differentiation, were assessed by a panel of six cytopathologists. Their diagnoses were recorded and then compared with the definitive diagnosis established by combining the cytologic findings with the results of intermediate filament typing of tumor cells in the smears using monoclonal antibodies specific for each filament type. The results show that use of these antibodies can markedly improve the accuracy of the cytologic diagnosis of tumor type as well as revise or prevent erroneous cytologic diagnoses in difficult cases. This pertains especially to the differential diagnoses of carcinoma versus malignant lymphoma, carcinoma versus malignant melanoma, carcinoma versus sarcoma and squamous carcinoma versus carcinoma of simple epithelia. Intermediate filament typing of tumor cells in aspirates as an objective histogenetic criterium makes the differential diagnosis of the difficult aspirates much more reliable and reproducible, provided that appropriate questions are asked, monoclonal antibodies with well-defined specificities are used and the antigenicity of the intermediate filaments in smears is preserved.

Adult↗