Biomedical subjects

L Y Shipova

Publications and source records attributed to L Y Shipova.

3 recordsLinked to original sources

PubMed1998

Inducible protein in rat hepatomas with expression alternative to alpha-fetoprotein.

The rat hepatoma cell line McA RH7777 was cloned into alpha-fetoprotein-producing (AFP+) and non-producing (AFP-) sublines. A monoclonal antibody (MAb A2/3) reacting with an antigen (Ag A2/3) present only in AFP- clones or AFP- cells in mixed clones was obtained. Ag A2/3 was absent from the liver of embryonic, fetal, newborn and adult rats, but it was present in gastric and intestinal mucosa of adult rats. Ag A2/3 was found to be a heavy metal-inducible protein: Cd2+ and Pb2+ strongly induced the expression of Ag A2/3 in vivo in the liver of adult rats, while xenobiotics and CCl4 were not active in this respect. In vitro Cd2+ and Pb2+ induced Ag A2/3 expression in several AFP+ clones, leading to a simultaneous marked decrease of AFP+ cells from such clones. The effect of Cd2+ in the induction of Ag A2/3 and suppression of AFP was reversible. SDS PAGE revealed one protein band with an m.w. close to 45,000, which was not sensitive to mercaptoethanol. Despite its inducible properties, Ag A2/3 was shown not to belong to metallothioneins, cytochrome P-450, glutathion-transferase or heat shock proteins families, well-known as being inducible cell stress proteins. Expression of Ag A2/3 could be one of the factors determining the high amplitude of AFP production by individual liver tumors. The nature of Ag A2/3 and its alternative expression with respect to AFP remain to be studied.

Animals↗

PubMed1985

AFP-synthesis induction in mouse hepatomas.

Highly differentiated, spontaneous hepatomas occurring in 13- to 15-month-old CBA mice were unable to produce embryo-specific serum protein alpha-fetoprotein (AFP). AFP synthesis, however, was induced in such tumors by minimal mechanical damage (small incisions of the tumors). On the 3rd day following surgery, AFP-positive cells were detected in two zones: (1) along the inflammatory shaft arising around the peripheral parts of incisions, and (2) near large blood vessels draining the damaged areas. It is suggested that mechanisms regulating AFP synthesis in highly differentiated hepatomas are similar to those of the normal liver and are related to local structural rearrangements.

Animals↗

PubMed1975

Immunohistochemical study of alpha-fetoprotein and serum albumin in the early postnatal period in mice.

The localization of the embryo-specific protein alpha-fetoprotein (alpha-FP) and serum albumin in the organs of mice in the first three weeks of life was studied. Using a reaction of immunofluorescence, alpha-FP was detected in the cytoplasm of the hepatocytes. During the first three days of life it was contained in the overwhelming majority of the hepatocytes, but in different concentrations. Then the fluorescence intensity and number of cells containing alpha-FP decreased sharply, and by the seventh to 12th day such cells remained chiefly close to the central veins. In a simultaneous determination of alpha-FP and albumin in the liver, cells containing only alpha-FP, alpha-FP and albumin, and only albumin were detected. With increasing age of the animals, the number of the latter cells increased. On slices of other organs, alpha-FP, albumin, and transferrin were present in the same structures of gamma-globulin.

Alpha-Globulins↗