Biomedical subjects

L Yu

Publications and source records attributed to L Yu.

At least 325 records · Page 18Linked to original sources

PubMed1998

Detection of Fas/APO-1 in six human urogenital malignant cell lines with flow cytometry.

OBJECTIVE: To investigate the expression of Fas/APO-1 in urogenital tumor cell lines. METHODS: With direct immunofluorescence, the expression of Fas/APO-1 in six urogenital malignant cell lines and one primary in vitro cultured normal renal fibroblast was detected by flow cytometry. RESULTS: Expression of Fas/APO-1 was detected in all six urogenital tumor cell lines, but with limited positive cell percentage and relatively lower fluorescence intensity, compared with expression of Fas/APO-1 in normal control of primary in vitro cultured renal fibroblast. CONCLUSIONS: Lower expression of Fas/APO-1 in urogenital malignant cell lines than that in normal cells might be the reason for occurrence and progression of urogenital malignant tumors.

Apoptosis↗

PubMed1998

Diagnosis and classification of hepatic echinococcosis by ultrasonography.

OBJECTIVE: To make an early and correct diagnosis of hepatic echinococcosis. METHODS: A total of 1092 patients with hepatic echinococcosis underwent operation in our hospital between 1984 and 1995. Of these patients, 427 (39.1%) were cases with complications, including secondary infection, rupture, obstructive jaundice, anaphylactic shock, disseminated implantation with resultant multiple echinococcosis and portal hypertension. The ultrasonic examination has been generally used in clinical practice for comprehensive evaluation which can dectedct the preoperative diagnostic accuracy rate. RESULTS: B-mode ultrasonography can be used not only to detect the location, dimension and pathological characteristics of hydatid cyst but also to show the pathological changes of various complications caused by echinococcosis. Seven patterns specific ultrasonic scanning images were revealed. In this series the diagnostic accuracy rate reached 98.8%. CONCLUSIONS: Ultrasonic examination is harmless to human body, and has been widely used in combination with immunological tests in clinical and epidemiological studies to detect the asymptomatic parasite carriers in early stage and to improve the preoperative diagnostic accuracy rate.

Echinococcosis, Hepatic↗

PubMed1998

[Treatment of ischemia in lower extremities by primary arterization in situ of Vana saphena magna].

In order to summarize the experience in the treatment of ischemic necrosis of lower extremities resulted from thrombotic occluded angittis, 15 cases were reported, which were treated by primary arterization in situ of V. Saphena magna. With a period of follow-up, 4-26 months on the average, it was found that symptoms in 14 cases were much allayed obviously, except 1 case with little relief. It suggested that primary arterization in situ of V. saphena magna could improve the circulation of the ischemic extremity rapidly without any influence of venous reflux.

Adult↗

PubMed1998

[Studies of meiotic origin of the extra chromosome 21 in Down syndromes detected by using (GT)n polymorphic DNA markers].

The polymorphics of two pericentric (GT)n sequences on the long arm of human chromosome 21 have been analyzed after PCR amplification, PAGE and Ag-staining for the first time in 50 Chinese Han people, and were used to detect meiotic origin of the extra chromosome 21 in Down syndromes. Six and 5 alleles were found in Chinese Han people for D21S215 and D21S120, respectively, with observed heterozygosities of 0.68 and polymorphic information content PIC, 0.67 and 0.65. For 17 Down syndromes whose parental origin of the extra chromosome 21 were known, meiotic origin of the extra chromosome 21 were determined in 16 cases, with 7 and 4 maternal meiosis I and II nondisjunction, 2 and 3 paternal meiosis I and II, respectively. The possible biological significance of the study on origin of the extra chromosome 21 has been discussed.

Chromosomes, Human, Pair 21↗

PubMed1998

[The effect of Epstein-Barr virus gene BHRF1 expression on the apoptotic resistance of nasopharyngeal carcinoma cells].

OBJECTIVE: To investigate the effect of EB virus gene BHRF1 expression on the apoptotic resistance of nasopharyngeal carcinoma cell. METHODS: Expression vector for BHRF1 was constructed and transfected into nsaopharyngeal carcinoma cell line, CNE2. The alteration of biologic behaviour of the transfected cells was tested after 60Co radiation. RESULTS: BHRF1 expression could inhibit the expression of proliferative cell nuclear antigen, redistribute cell cycle, decrease cell sensitivity to radiation, and enhance their survival ability. CONCLUSION: It is suggested that BHRF1 expression can increase the apoptotic resistance induced by radiation.

Animals↗

PubMed1998

[Hyperinsulinemia and insulin resistance in nondiabetic first-degree relatives of patients in familial NIDDM pedigrees].

OBJECTIVE: To study plasma insulin level and insulin sensitivity of nondiabetic first-degree relatives of patients in familial non-insulin-dependent diabetes mellitus (NIDDM) pedigrees. METHODS: Standard oral glucose tolerance test(OGTT) was conducted in 55 healthy controls and 97 first-degree relatives of NIDDM probands in familial NIDDM pedigrees. Plasma glucose and plasma insulin were determined at all phases after oral glucose load. Moreover, we calculated the glucose/insulin area ratio under the curve of OGTT (SGI) and the insulin sensitivity index (ISI) in all subjects to estimate their insulin sensitivity. RESULTS: In nondiabetic first-degree relatives, SGI and ISI were significantly decreased (P < 0.01), and plasma insulin was increased at 0, 30, 60, 120 minutes after administration of oral glucose load (P < 0.05 and P < 0.01), as compared with the controls, in matched age, body mass index (BMI) and proportion of sex. In both first-degree relatives and the insulin controls, SGI was positively correlated with ISI (r = 0.39, 0.41, respectively, P < 0.01) and both SGI and ISI were inversely correlated with BMI (r = -0.38, -0.39; -0.32, -0.25, respectively, P < 0.01). Moreover, FINS in the two groups was positively correlated with BMI (r = 0.30, 0.34, respectively, P < 0.05). CONCLUSION: In nondiabetic first-degree relatives of patients in familial NIDDM pedigrees, plasma insulin level was increased and insulin sensitivity was decreased as compared with the healthy controls. It suggests that they suffered from hyperinsulinemia and insulin resistance.

Adult↗

PubMed1998

[The diagnosis and therapy of tinnitus].

Selection criteria for the therapeutic strategy are provided by a classification on the basis of the following points: 1. can be masked by a sound, 2. response to parenteral lidocaine. This classification was used to treat 119 patients with tinnitus. Masking is the first therapy for tinnitus. If masking had no effect, lidocaine test were undergone. The patient who has a positive response to lidocaine were treated by antiepileptic drugs. Patients with negative response to lidocaine negative were treated by suppressant. The prognosis for patients with positive masking effect was more better than the negative one. The mechanisms of tinnitus were discussed.

Anticonvulsants↗

PubMed1998

[Clinical and genetic analysis of a severe vWD pedigree].

OBJECTIVE: To investigate clinical features and analyse defect gene transmission in a severe vWD pedigree. METHODS: Phenotypes of a severe hemorrhagic pedigree including 26 family members of four generations were diagnosed with vWF multimer banding and ELISA. Defective gene transmission among the family members was investigated with a series of RFLP and VNTR loci within vWF gene. RESULTS: For the proband, plasma vWF:Ag was less than 2%, and multimer banding was negative. Gene analysis showed that she carried 2 different defective vWF haplotypes from her parents. The family members carrying one of the defective haplotypes showed normal or slight decrease of vWF:Ag and normal multimer banding pattern. CONCLUSION: 1. The proband could be regarded as a putative compound heterozygote of type 3 vWD. The defect vWF gene carried by heterozygote family members could be compensated by a normal allele. 2. DNA analysis based on polymorphism markers within vWF gene has practical values in genetic consulting of severe vWD pedigrees.

Adolescent↗

PubMed1998

[A preliminary study on the in vitro eukaryotic expression of human factor VIII cDNA].

OBJECTIVE: To evaluate the expression efficiency of a cDNA sequence of human clotting factor VIII (4.7 kb, B domain-deleted) in in vitro systems. METHODS: After insertion of the cDNA into several mammalian expression vectors, such as retroviral vector pMSCV, EB virus-based vector pGRE5.2/EBV and eukaryotic expression plasmid pCI, the expression of these constructs were tested in a variety of cells. RESULTS: All the three kinds of constructs-pCI-VIII, pGRE5.2/EBV-VIII and pMSCV-VIII were able to direct FVIII synthesis in NIH3T3, Hela and Bosc23 cells, respectively, while the pMSCV-VIII and pGRE5.2/EBVVIII produced relatively high levels of FVIII activity (up to 0.7 units/ml and 2.0 units/ml from 24 h to 48 h, respectively, after transfection with lipofectamine). The three forms of pMSCV-VIII vector worked in a similar efficacy in Bosc 23 cells, but this function was not detected in NIH3T3, psi-Crip and GP + E86 as well as 32DC13 cells in a transient transfection assay. Moreover, the NIH3T3 and 32DC13 cells infected with culture supernatant from pMSCV-VIII transfected-Bosc23 cells (as packaging cells) unexpectedly did not produce detectable FVIII activity. CONCLUSION: Apart from the design and construction of vectors, target cell selection may play a crucial role in the efficient expression of the FVIII cDNA.

3T3 Cells↗

PubMed1998

[Linkage analysis of vWD family by multi-PCR assay of microsatellite DNA in vWF gene].

OBJECTIVE: To set up a convenient method for analysis of the amplified fragment length polymorphism (amp-FLP) of two microsatellite DNA in vWF gene. METHODS: Multi-PCR followed by denatured PAGE and silver stain were used to analyze the amp-FLP of two locus (A and B) on one gel. DNA samples from 112 normal people and two vWD families in the Shanghai area were assayed. RESULTS: 1. seven and 5 types of amp-FLP were identified on loci A and B, respectively. The heterozygote rates were 75% and 74%, respectively. 2. Haplotypes A2/B3 and A4/B3 were found to link with defective vWF genes in the two vWD families, respectively. CONCLUSION: 1. Multi-PCR is a fast and practical method to carry out family analysis of inherited diseases. 2. nt1980-1990 and nt2215-2380 of vWF gene are two ideal genetic labels in linkage study and hereditary consultation of vWD family.

Child↗

PubMed1998

[Reconstruction of transcriptional regulatory elements of human alpha-fetoprotein gene].

OBJECTIVE: To construct vectors that express therapeutic genes specifically in hepatocellular carcinoma, the TREs (Tissue-specific transcriptional regulatory elements) of natural human alpha-fetoprotein (AFP) was reconstructed. METHODS: Using subcloning techniques, the -870 bp(-)-3.06 kb fragment of the natural human AFP TREs was deleted to construct an artificial AFP(e/p) TREs which contains the "core" promoter and the -3.06 kb(-)-5.1 kb fragment of natural human AFP TREs that contains enhancer activity. RESULTS: By using chloramphenicol acetyltransferase (CAT) assay, the artificial AFP(e/p) TREs showed an activity 1.8 fold as high as that of the natural AFP TREs in an AFP positive human hepatocarcinoma cell line HepG2, and it also remained to be tissue-specific since there was no activity was detected in an AFP negative human larynxcarcinoma cell line Hep2. CONCLUSIONS: These results indicate that the artificial AFP(e/p) TREs we have constructed has higher activity than the natural AFP TREs and it also remains its hepatocarcinoma specificity. The reconstructed AFP(e/p) TREs is suitable for constructing of gene therapy vector with hepatocarcinoma expression specificity.

Carcinoma, Hepatocellular↗

PubMed1998

[On the best time for harvesting Dendrobium candidum Wall. ex Lindl].

OBJECTIVE: To explore the theory on the best time for harvesting Dendrobium candidum so that the production of the herb can be directed more effectively. METHOD: Based on a component analysis in combination with mathematical calculation, a synthetical appraisal on the yield, contents of effective components and efficacy of D. candidum in different growing periods has been made, and different appraisal indexes and synthetical harvesting indexes have been worked out. RESULT: The harvesting indexes from the first year to the fourth year are as follows: 5.69, 9.54, 17.9, 22.2 (curing throat and eye diseases); 11.2, 14.6, 21.6, 17.6(strengthening immunity); 8.44, 12.1, 19.7, 19.9(the above-mentioned indexes and yield factor considered together). CONCLUSION: The best time for harvesting D. candidum is in the fourth year for curing throat and eye diseases; in the third or first year for strengthening immunity; and in autumn of the third year if the above-mentioned indexes and yield factor are to be considered together.

Alkaloids↗

PubMed1998

[Radioimmunoimaging of bladder tumor with the intravesical administration of technetium -99m labelled monoclonal antibody].

OBJECTIVE: To make radioimmunoimaging of bladder tumor simple, safe and reliable. METHODS: Anti-human bladder carcinoma cell line BIU-87 monoclonal antibody-BDI-1 was labelled with technetium -99m by direct mercaptoethanol reducing method. RESULTS: The labelling rate was 69.6% and the radiochemistry pruity was higher than 95%. 33 patients received radioimmunoimaging of bladder tumor with the intravesical administration of technetium -99m labelled monoclonal antibody. The false positive rate was zero. 88.5% of all 29 bladder cancer patients had got positive immunoscintigraphy results, and 65.2% of them were confirmed by cystoscopic results. Both primary and recurrent tumor could be detected clearly and the smallest tumor detected was 0.5 cm in diameter. There were some differences between the radioimmunoimaging and cystoscopic results in 7 of 11 multitumor cases, which included tumor size, number and location. CONCLUSION: It is required to use more suitable isotopes and/or monoclonal antibodies to increase its sensitivity.

Administration, Intravesical↗

PubMed1998

[Apoptosis induced by ionizing radiation in TCC cell lines, EJ and BIU-87].

OBJECTIVE: To study the effect of ionizing radiation on the living status of TCC cell lines, EJ and BIU-87. METHODS: Different doses of X-ray were used on cell lines, EJ and BIU-87, and the cells were observed microscopically by fluorescent staining(EB and AO). RESULTS: The number of normal living cells declined and apoptotic as well as necrotic cells elevated with increase of radiation doses except for doses equal to or below 100 cGY, Apoptotic and necrotic cells increased as postradiation time delayed. CONCLUSION: The ionizing radiation may induce TCC apoptosis and necrosis.

Apoptosis↗

PubMed1998

[Retroviral-mediated transcriptional regulatory elements of alpha-fetoprotein gene directed specific expression of interleukin-2 in murine hepatoma].

OBJECTIVE: To target expression of interleukin-2 in alpha-fetoprotein (AFP) positive hepatocellular carcinoma. METHODS: A retroviral vector pDOR-mAFP-IL2 was constructed by coupling the transcriptional regulatory elements (TREs) of murine AFP gene with interleukin-2 cDNA in a retroviral vector. RESULTS: Subsequent to retroviral infection, expression of IL-2 could be detected in an AFP positive murine hepatoma cell line Hepa1-6 but not in psi 2, an AFP negative murine fibroblast cell line. CONCLUSIONS: Our results indicate that specific expression of IL-2 in hepatoma cell can be achieved by using of AFP TREs to control the expression of IL-2, and the retroviral vector which we have constructed may be used for further experimental studies on in vivo gene therapy.

Animals↗

PubMed1998

[A monoclonal antibody that reacts with an antigen on both normal and malignant prostate cells].

OBJECTIVE: To study the YDPC--a monoclonal antibody that reacts with an antigen on both normal and malignant prostate cell. METHOD: A murine monoclonal antibody (MAb) designated YDPC was generated by treating the primed spleen cells with leucine methyl ester (LeuOMe) following immunization of mice with prostate cancer cell line PC-3M. RESULT: YDPC reacted uniformly with all adenocarcinomas of the prostate examined. It also reacted with the surface antigen on normal prostate epithelial cells and on cells from benign prostate hyperplasia. YDPC reacted with a limited number of normal tissues including renal tubules, adrenal and sweat gland. In addition, three lymph nodes metastasis and one bladder metastasis were found to be strongly positive, while four transitional cell carcinomas of the prostate negative. Preliminary experiments showed that this antibody does specifically localize to prostate cancer xenografts in nude mice. CONCLUSION: YDPC reacts with a differentiation antigen and this antigen continues to be expressed on all adenocarcinomas of the prostate. This antibody may be useful in the diagnosis of or therapy for prostate cancer.

Adenocarcinoma↗

PubMed1998

[Apoptosis of renal cell carcinoma and apoptotic related genes bcl-2 and bax].

OBJECTIVE: To explore the mechanism of renal cell carcinoma (RCC). METHOD: We detected the apoptotic status and the expression of apoptotic related gene bcl-2 and bax protein in 48 cases of RCC with TdT-mediated dUTP-biotin nick end labeling (TUNEL) and immunohistochemistry methods. RESULT: 22 cases showed low expression of bcl-2 protein with an average apoptotic index (AI) of 1.35 and 26 cases had high expression of bcl-2 with an AI of 1.04 (P < 0.05). 34 cases showed low expression of bax protein with average AI of 1.03 and 14 cases had high expression of bax protein with an average AI of 1.63 (P < 0.01). The stronger bcl-2 expression, the weaker bax expression and the less number of apoptotic cells. CONCLUSION: both bcl-2 and bax take part in the occurrence and progression of RCC.

Adolescent↗

PubMed1998

[Expression and significance of Fas/APO-1 and bcl-2 protein in renal cell carcinoma].

OBJECTIVE: To investigate the role of Fas/APO-1 and bcl-2 in occurrence and progression of renal cell carcinoma. METHOD: Immunohistochemistry method was used to detect the expression of Fas/APO-1 and bcl-2 protein in 35 cases of renal cell carcinoma tissues and 26 cases of normal renal tissues. RESULT: The expression rate of Fas/APO-1 protein in renal cell carcinoma tissues was 57.14%, significantly lower than that in normal renal tissues (84.62%) (P < 0.05), and the intensity of expression was also lower. The expression rate of bcl-2 protein reached 80.00%, much higher than that in normal renal tissues (53.85%) (P < 0.05). CONCLUSION: Both Fas/APO-1 and bcl-2 take part in the occurrence and progression of renal cell carcinoma.

Adult↗