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Biomedical subjects

L Zapatero

Publications and source records attributed to L Zapatero.

15 recordsLinked to original sources
PubMed2003

Humoral immune responses induced by Kudoa sp. (Myxosporea: Multivalvulida) antigens in BALB/c mice.

The majority of Kudoa species infect the somatic muscle of fish establishing cysts. As there is no effective method to detect infected fish without destroying them these parasited fish reach the consumer. This work was developed to determine whether this parasite contains antigenic compounds capable of provoking an immune response in laboratory animals, in order to consider the possible immunopathological effects in man by the ingestion of Kudoa infected fish. BALB/c mice were injected by the subcutaneous route with the following extracts suspended in aluminium hydroxide: group 1 (black Kudoa sp. pseudocyst extract), group 2 (white Kudoa sp. pseudocyst extract), and group 3 (non-infected hake meat extract). Specific antibody levels were measured by ELISA against homologous and heterologous antigens. The highest responses were obtained from the black Kudoa sp. pseudocyst extract (group 1). The low optic density levels detected in group 3 proved that the results obtained in groups 1 and 2 were a consequence of the parasitic extract injection. The IgG1 was the predominant subclass. IgE detected in groups 1 and 2 showed the possible allergenic nature of some of the components of the parasitic extract. High IgA levels and medium IgG2a and IgG3 levels were obtained in groups 1 and 2. Low IgG2b responses were shown. No cross-reactions between Kudoa sp. pseudocyst extracts and the non-infected hake meat extract were observed.

Animals↗
PubMed2001

Allergy to grape: a case report.

We report a case of a 5-year-old child who suffered an oral allergy syndrome and lip angiedema after eating grapes. We obtained a positive prick test with commercial grape extract and a positive prick-by-prick test with pulp and peel of fresh white grape (Moscatel variety) and pulp and peel of blue grape. Sodium dodecyl sulphate-polyacrylamide gel electrophoresis followed by immunoblotting revealed specific immunoglobulin E (IgE) antibodies in the patient's serum against a 94,000 molecular-weight antigenic band. Lip open challenge was positive.

Angioedema↗
PubMed1999

Allergy to an occupational allergen (Sapelli wood) in a child.

The case is presented of a child who developed rhinoconjunctivitis, angioedema and asthma by sensitization to Sapelli wood, which was used in his father's carpentry. Positive skin-prick test, high levels of specific immunoglobulin E by ELISA and a positive conjunctival challenge test suggest a type I hypersensitivity mechanism to this wood. This is the first case report of sensitization to Sapelli wood and it confirms that occupational allergen exposure as Sapelli wood may also cause sensitization in a child.

Allergens↗
PubMed1993

Epidemic asthma in Cartagena, Spain, and its association with soybean sensitivity.

We conducted a matched case-control study to assess the association between epidemic asthma and soybean sensitivity in Cartagena, Spain. In skin tests, 81% of the cases and no control subjects were positive to the commercial soybean allergen; 87% of the cases and 12% of the controls were positive to the prepared soybean allergen; and 69% of the cases and 6% of the controls showed high levels of specific immunoglobulin E antibodies (odds ratio = 10; 95% confidence interval = 1.4-433). The association remained strong after adjustment for total immunoglobulin E and for 28 other allergens. The results indicate that soybean sensitivity may be causing asthma epidemics in Cartagena.

Adolescent↗
PubMed1992

Purification and characterization of major inhalant allergens from soybean hulls.

Proteins responsible for respiratory allergy to soybean have been purified from an extract of soybean hulls. The purification procedure combined size exclusion and reverse-phase HPLC. Two pure glycoproteins (S1 and S2) exhibiting IgE-binding ability, as demonstrated by immunoblotting and ELISA techniques, were obtained. Both proteins displayed low molecular weight values on SDS-PAGE (S1, 7.0 kD; S2 7.5 kD). Protein S1 showed charge microheterogeneity, rendering two bands at pH 6.1-6.2 on IEF, whereas S2 showed a single band at pH 6.8. Amino acid composition analyses revealed a strong homology between S1 and S2 and, as a characteristic feature, a high percentage of hydrophobic residues, mainly leucine and isoleucine. Concerning the allergenic activity, both proteins were recognized by the specific IgE from 95% of patients who suffered asthma attacks during the asthma outbreaks of 1987 and 1988 in Cartagena (Spain), caused by soybean dust. Besides, proteins S1 and S2 were able to, separately, inhibit up to 75% the binding of specific IgE to the whole extract. Moreover, purified proteins totally crossreacted, even though protein S2 seemed to be slightly more active in all the immunochemical techniques employed. Results presented allow us to conclude that both proteins are isoallergens and to name them as Gly m IA (protein S2) and Gly m IB (protein S1), according to the IUIS-allergen nomenclature system.

Allergens↗
PubMed1991

Identification of soybean proteins responsible for respiratory allergies.

Serum samples from 32 patients who suffered attacks during the asthma outbreaks of 1987 and 1988 in Cartagena, Spain, supposedly caused by soybean dust, were studied. At least 90% had specific IgE to shell components and only 13% showed specific IgE to shell-depleted soybean grains. A control group of 32 patients who also suffered asthma attacks but on different days from those of the outbreaks were negative. The shell's most important allergen with an apparent molecular weight of 8 kDa was not present in shell-depleted grains. This allergen as well as other less important shell allergens may be different from the allergens already identified by using serum from patients suffering food allergy to soya.

Allergens↗
PubMedDate not supplied

Allergic reactions due to ibuprofen in children.

Ibuprofen is 2-(4-isobutyl-fenyl)-propionic acid, which belongs to the same group of nonsteroidal antiinflammatory drugs (NSAIDs) as naproxen and ketoprofen. We present two instances of adverse reaction to pediatric ibuprofen, an acute urticaria and a fixed drug eruption, with tolerance to acetylsalicylic acid (ASA) and acetaminophen, in what seems to be hypersensitivity to the propionic acid group. Although these reactions are very rare and ibuprofen is still very safe, we think it is important to know about the possible side effects in order to recognize and treat them when they occur.

Anti-Inflammatory Agents, Non-Steroidal↗
PubMedDate not supplied

A study of allergy to penicillin antibiotics in 1995 in the Child Allergy Department of the Gregorio Marañón University hospital.

We present 219 cases of adverse reactions to penicillin and related antibiotics during 1995 in the Child Allergy Department of Gregorio Marañón University Hospital. Amoxicillin was the most frequent cause of the reaction. The most common clinical symptom was urticaria followed by angio-edema and other characteristic exanthemas. A diagnosis of allergy to penicillin and related drugs was only made in 20 cases in which the predominant symptomatology was urticaria and the antibiotic most commonly involved was again amoxicillin. Diagnosis was confirmed by a skin test, specific IgE determination and controlled provocation. In 3 of the 20 cases diagnosis was made in the reassessment or second study, which was carried out in all cases in which more than one year had passed between the reaction and the initial allergological study.

Child↗