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Lars Linsen

Publications and source records attributed to Lars Linsen.

2 recordsLinked to original sources

Discrete Sibson interpolation.

Natural-neighbor interpolation methods, such as Sibson's method, are well-known schemes for multivariate data fitting and reconstruction. Despite its many desirable properties, Sibson's method is computationally expensive and difficult to implement, especially when applied to higher-dimensional data. The main reason for both problems is the method's implementation based on a Voronoi diagram of all data points. We describe a discrete approach to evaluating Sibson's interpolant on a regular grid, based solely on finding nearest neighbors and rendering and blending d-dimensional spheres. Our approach does not require us to construct an explicit Voronoi diagram, is easily implemented using commodity three-dimensional graphics hardware, leads to a significant speed increase compared to traditional approaches, and generalizes easily to higher dimensions. For large scattered data sets, we achieve two-dimensional (2D) interpolation at interactive rates and 3D interpolation (3D) with computation times of a few seconds.

Algorithms↗

Visual analysis of gel-free proteome data.

We present a visual exploration system supporting protein analysis when using gel-free data acquisition methods. The data to be analyzed is obtained by coupling liquid chromatography (LC) with mass spectrometry (MS). LC-MS data have the properties of being nonequidistantly distributed in the time dimension (measured by LC) and being scattered in the mass-to-charge ratio dimension (measured by MS). We describe a hierarchical data representation and visualization method for large LC-MS data. Based on this visualization, we have developed a tool that supports various data analysis steps. Our visual tool provides a global understanding of the data, intuitive detection and classification of experimental errors, and extensions to LC-MS/MS, LC/LC-MS, and LC/LC-MS/MS data analysis. Due to the presence of randomly occurring rare isotopes within the same protein molecule, several intensity peaks may be detected that all refer to the same peptide. We have developed methods to unite such intensity peaks. This deisotoping step is visually documented by our system, such that misclassification can be detected intuitively. For differential protein expression analysis, we compute and visualize the differences in protein amounts between experiments. In order to compute the differential expression, the experimental data need to be registered. For registration, we perform a nonrigid warping step based on landmarks. The landmarks can be assigned automatically using protein identification methods. We evaluate our methods by comparing protein analysis with and without our interactive visualization-based exploration tool.

Chromatography, Gel↗