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Leander Zickler

Publications and source records attributed to Leander Zickler.

2 recordsLinked to original sources

Shack-Hartmann-Based wavefront analyzer for calibrating excimer lasers.

PURPOSE: Currently, refractive surgical excimer laser systems are calibrated by ablating plastic lenses, which are measured by lensometer and analyzed by a technician. The accuracy of this method is approximately 0.25 diopters (D) in sphere and cylinder power. Theoretically, objective calibration using wavefront technology would be significantly more accurate, thereby improving surgical outcomes. This study describes a Shack-Hartmann-based instrument, which has been developed to measure ablated plastic lenses for calibration and quality control of the excimer laser. METHODS: A calibration instrument comprising an LED source at 640 nm, a lenslet array, beam-guiding optics, and a CCD camera was designed to perform full wavefront analysis. The measurement plane is conjugate to the lenslet array plane, and the diameter of the pupil is 5 mm. Accuracy was determined by measuring a set of well-calibrated spherical and cylindrical glass lenses. Plastic lenses were ablated, and high-precision measurements were performed by surface profile scanner. RESULTS: In the power range of -6.00 to +4.00 D, repeatability exceeded 0.01 D, accuracy of measurement exceeded 0.04 D, and 10 for the axis of cylinder lenses. The measurement of excimer-ablated plastic lenses agreed with high-precision surface profile scanner measurements within 0.10 D, and repeatability exceeded 0.01 D. CONCLUSIONS: Wavefront technology-based, high-precision measurement of calibration lenses can more accurately set the energy of the excimer laser, which enhances the accuracy of refractive laser correction. In automating calibration, the new instrument removes operator subjectivity and decreases the time needed for calibration.

Calibration↗

Second-harmonic imaging of cornea after intrastromal femtosecond laser ablation.

Nonlinear laser scanning microscopy is widely used for noninvasive imaging in cell biology and tissue physiology. However, multiphoton fluorescence imaging of dense, transparent connective tissue (e.g., cornea) is challenging since sophisticated labeling or slicing is necessary. High-resolution, high-contrast second harmonic generation (SHG) imaging of corneal tissue based on the intrinsic structure of collagen is discussed. The three-dimensional corneal ultrastructure in depths up to hundreds of microns can be probed noninvasively, without any staining or mechanical slicing. As an important application of second harmonic imaging in ophthalmology, the modification of corneal ultrastructure using femtosecond laser intrastromal ablation is systematically investigated to evaluate next-generation refractive surgical approaches.

Animals↗