Biomedical subjects
Publications and source records attributed to Ledao Li.
The qualitative and quantitative aspects of several novel methods for drug-protein binding studies, which include chromatography, capillary electrophoresis, mass spectrometry, nuclear magnetic resonance, cloud-point extraction and microdialysis as well as those of the traditional approaches such as equilibrium dialysis and ultrafiltration are reviewed. The advantages and limitations of these methods are discussed.
Three methods of coupling development and elution were studied in this paper. (1) A new mode of solvent supplementation and eluate collection was developed for descending development. By using a new distributor and collector in descending development, components can be separated and eluted continuously. (2) The same effect can be realized with a slope distributor [Su et al., J. Planar Chromatogr. 14 (2001) 203] and a collector by horizontal development. (3) In-situ elution can be used to treat a developed silica plate, which can elute the separated components to the receptor without scraping them off. These three methods can be used individually, and the in-situ elution can be used with other modes of development.