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Biomedical subjects

Li Li

Publications and source records attributed to Li Li.

At least 181 records · Page 10Linked to original sources
PubMed2006

[Studies on chemical constituents in roots of Heracleum rapula].

OBJECTIVE: To study the chemical constituents in roots of Heracleum rapula. METHOD: The constituents were isolated by column chromatography on silica gel and ODS, and identified by spectroscopic methods. RESULT: Eight compounds, xanthotoxol (I), 8-geranyloxypsoralen (II), (+)-marmesin (III), beta-sitosterol (IV), stigmasterol (V), oleanolic acid (VI), ferulic (VII), scopoletin (VIII) were isolated and identified. CONCLUSION: Compounds IV, V, VI, VII, VIII were isolated from this plant for the first time.

Coumaric Acids↗
PubMed2006

[Typical and atypical features of focal nodular hyperplasia of the liver on helical CT images].

BACKGROUND & OBJECTIVE: Focal nodular hyperplasia (FNH) is a rare hepatic neoplastic lesion. This study was to evaluate the typical and atypical features of FNH of the liver on helical computed tomography (CT) images to improve the diagnosis accuracy. METHODS: Helical CT images of 32 patients with 37 lesions were reviewed in Cancer Center, Sun Yat-sen University retrospectively, who were confirmed as FNH by histopathologic or clinic examinations. The number, size, margin status, density, enhancing type, presence of a center scar, and presence of a pseudocapsule of FNH lesions were analyzed. RESULTS: The largest diameters of 14 lesions were < or = 3 cm, of 23 lesions were > 3 cm; 20 lesions were subcapsular; pseudocapsule was presented in 7 lesions with the largest diameters of > 3 cm; 22 lesions had center scars, among them, the largest diameters of 20 lesions was > 3 cm. All lesions were hyperattenuated to the liver in hepatic arterial phase; 31 lesions were enhanced homogeneously in hepatic arterial phase; abnormally thickened vessels were presented at the border of 17 lesions. CONCLUSIONS: Typical helical CT features of FNH include homogeneous enhancement in hepatic arterial phase, presence of central scar, and delayed enhancement. Atypical helical CT features of FNH include heterogeneous enhancement in hepatic arterial phase, absence of central scar, and presence of pseudocapsule. The various CT features are related to the pathologic type and the largest diameter of the lesion.

Adenoma↗
PubMed2006

[Developing of a new multicolor-fluorescent labeled STR amplification kit].

OBJECTIVE: To develop a PCR-based STR system for genotyping of 18 loci (Amelogenin, D3S1358, vWA, FGA, D8S1179, D21S11, D18S51, D5S818, D13S317, D16S539, TH01, TPOX, CSF1PO, D7S820, D2S1338, D19S433, D12S391 and D19S253). METHODS: By using primers labeled with four color fluorescent (FAM, HEX, TAMRA and ROX), two multiplex amplification reaction systems were developed to genotype Amelogenin and 17 STR loci. RESULTS: Amelogenin and these 17 STR loci were genotyped successfully in different kinds of biological samples by the kit. CONCLUSION: The STR amplification kit developed in our study gives a new approach to genotype these 18 loci in a efficient, steady and reliable way.

Alleles↗
PubMed2006

Prevention of retinoic acid-induced early craniofacial abnormalities by folinic acid and expression of endothelin-1/dHAND in the branchial arches in mouse.

Prevention of retinoic acid-induced craniofacial abnormalities by folinic acid, and endothelin-1 (ET-1)/dHAND protein and mRNA expression were investigated in mouse embryos using the whole embryo culture, streptavidin-biotin peroxidase complex method, and whole-mount in situ hybridization. In the whole embryo culture, 1.0 and 0.1 mm-folinic acid dose dependently prevented branchial region malformations and decreased defects by 93 % and 77 %, respectively. Folinic acid at concentrations of 1.0 and 0.1 mm significantly increased ET-1 and dHAND protein expression levels compared to retinoic acid-exposed values in embryonic branchial areas. Folinic acid also increased ET-1 and dHAND mRNA levels in the same region. The present results suggest that folinic acid may prevent retinoic acid-induced craniofacial abnormalities via increasing ET-1 and dHAND levels in the branchial region during the organogenic period.

Animals↗
PubMed2006

[Prognostic significance of lymphocyte function associated anti-gen-3 (CD58) in childhood B cell-acute lymphocytic leukemia].

This study was aimed to investigate the value of CD58 in evaluation of early therapeutic effect on childhood B-ALL. The expression features of CD58 in 135 cases of childhood B-ALL were analyzed by four-color flow cytometry; MRD detection protocol for B-ALL using CD58/CD10/CD34/CD19 combination was established; the correlation between the expression features of CD58 and MRD detection was analyzed for the early therapeutic response in childhood B-ALL. The results showed that the mean value of CD58 MFI in 135 cases of B-ALL was 113.08 +/- 63.33, which was significantly higher than that in 15 cases of normal bone marrow controls (14.68 +/- 5.26, P < 0.01). In addition, CD58 was over expressed in 51.9% (70/135) of B-ALL patients, indicating that CD58 could be an effective marker in MRD detection. The CD58/CD10/CD34/CD19 was the second most effective combination next to TdT/CD10/CD34/CD19 in B-ALL MRD detection with flow cytometry. Meanwhile, the positive rate of MRD detection by flow cytometry was significantly lower in CD58 over expression group (P < 0.05). It is concluded that CD58 may be used as an indicator for detection of MRD in B-ALL patients, which would enrich the combination of MRD detection. The CD58 over expression may be considered as a marker of a favorable prognosis in childhood B-ALL.

Biomarkers, Tumor↗
PubMed2006

[Role of interleukin-1beta in regulating human cultured endometrial cell MMP-9 and TIMP-3 expressions in the mid-secretory phase].

OBJECTIVE: To investigate the role of interleukin-1beta (IL-1beta) in regulating the expressions of matrix metalloproteinase-9 (MMP-9) and tissue inhibitor of matrix metalloproteinase-3 (TIMP-3) in cultured human endometrial cells of the mid-secretory phase. METHODS: Human endometrial cells of the mid-secretory phase cultured in the absence of steroid hormones for 24 h were stimulated with rhIL-1beta at concentrations ranging from 50 to 1,000 U/ml for 48 h. The expressions of MMP-9 and TIMP-3 protein in the cells were detected by flow cytometry using immunofluorescent method. RESULTS: MMP-9 was expressed at the level of 1,491.38-/+68.95 in the control group, and at 1,592.40-/+47.57, 1,702.63-/+75.31, 1,994.49-/+52.98, and 2,347.58-/+45.87 in response to cell treatment with IL-1beta at 50, 100, 500, and 1,000 U/ml, respectively, suggesting that IL-1beta significantly increased MMP-9 expression in a dose-dependent manner (P<0.05). The expression level of TIMP-3 was 1,643.31-/+61.29 in the control group, and was 1,597.27-/+49.07, 1,443.93-/+81.23, 1,343.28-/+54.80, and 1,157.85-/+47.95 in 50, 100, 500, and 1,000 U/ml IL-1beta groups, respectively, suggesting that IL-1beta decreased TIMP-3 expression dose-dependently (P<0.05). CONCLUSIONS: IL-1beta significantly upregulated the secretion of MMP-9 but downregulated the secretion of TIMP-3 in human endometrial cells of the mid-secretory phase, resulting in extracellular matrix decomposition to facilitate the invasion by extravillous cytotrophoblasts.

Adult↗
PubMed2006

[Photodynamic effect of hematoporphyrin monomethyl ether on ovarian cancer cell line SKOV3].

BACKGROUND & OBJECTIVE: Photodynamic therapy (PDT) is a new treatment choice for ovarian carcinoma. Hematoporphyrin monomethyl ether (HMME) is a novel photosensitive reagent developed in China. This study was to investigate the photodynamic effect of HMME-based PDT on human epithelial ovarian cancer cell line SKOV3. METHODS: After an incubation with 30 microg/ml HMME for different time, the fluorescent image and intracellular location of HMME in SKOV3 cells were observed under a fluorescent microscope and laser scanning confocal microscope (LSCM). After being treated with different doses (5-50 microg/ml) of HMME and irradiated with different optical doses (1.5-12 J/cm(2)) of laser, the survival rate of SKOV3 cells was measured by MTT assay. Mechanisms of cell death during PDT was determined by Annexin V/PI double staining technique and analyzed by flow cytometry. RESULTS: Red fluorescence appeared shortly after administration of HMME and localized in cytoplasm; intracellular fluorescence intensity reached the peak after 3 h. High concentrations of HMME alone had cytotoxicity to SKOV3 cells, while laser irradiation alone had no effect on cell survival. Survival rate of SKOV3 cells was gradually decreased along with the increase of HMME concentration and laser dose, but such a trend diminished when HMME concentration reached 40 microg/ml. After treatment of HMME, the dead cells were predominantly necrosis cells. CONCLUSION: HMME has a photodynamic effect on SKOV3 cells.

Cell Line, Tumor↗
PubMed2006

[A randomized comparative trial of three combined regimens containing cisplatin for treatment of advanced non-small cell lung cancer].

BACKGROUND & OBJECTIVE: Vinorelbine (NVB), paclitaxel (TAX), and gemcitabine (GEM) are first-line chemotherapeutic drugs in the treatment for non-small cell lung cancer (NSCLC) currently. There are many domestically retrospective studies to compare efficacies and adverse reactions among these three regimens, all of which has composed of cisplatin (DDP). This randomized study was to investigate the efficacies and toxicities of these three regimens in NSCLC treatment, in order to choose a feasible regimen for NSCLC patients. METHODS: A total of 276 NSCLC patients were randomly assigned to a regimen of NP (NVB plus DDP ), or TP (TAX plus DDP), or GP (GEM plus DDP). Efficacies and toxicities were analyzed and compared after two cycles. RESULTS: The response rates were 42.3% (41/97) in NP arm, 43.0% (40/93) in TP arm, and 43.4% (36/83) in GP arm, and complete remission rates were 1.0% (1/97), 2.2% (1/93) and (0/83) respectively, without significant difference. The median survival time, disease-free survival time and 1-year survival rate were 8.5 months, 4.1 months and 31.9%; 8.8 months, 3.8 months and 33.3%, and 9.2 months, 3.9 months, 31.3%, respectively in NP, TP and GP arms without significant difference. The major adverse reactions were stage 3 to 4 myelo-suppression, nausea/vomiting, fatigue and phlebitis. There were highest incidences of leucopenia (42.2%), neutropenia (36.2%) and lowest incidence of thrombocytopenia (53.0%) in GP arm compared to NP arm (77.8%, 67.7%, 12.1%) or TP arm (71.0%, 57.0%, 13.0%) statistically (P<0.01 for all). The rates of nausea/vomiting in GP arm (16.8%) and TP arm (25.8%) were significantly lower than in NP arm (41.4%)(P<0.05); and the rate of fatigue in GP arm (38.5%) or phlebitis in NP arm was most frequent among the three arms (P=0.000, 0.008 respectively). CONCLUSIONS: There is no significant difference in short-term efficacy of chemotherapy regimen NP, TP and GP. TP and GP regimens, both of which possesses its own advantage, are more tolerable than NP regimen which has the most side effects.

Adult↗
PubMed2006

[Study on the chemical constituents of Mitragyna rotundifolia].

Twelve compounds were isolated from the EtOAc-soluble and ButOH-soluble portions of the EtOH extract from the bark of Mitragyna rotundifolia. These compounds were identified by their spectral data as dauricine (1), 3,4-dihydroxybezoic acid (2), beta-sitosterol (3), scopleton (4),3,4,5-trimethyoxyphenol-1-glucopyranoside (5), taraxerol (6), 4-hydroxy-3-methyloxybenzoic acid (7), 3-hydroxy-4-methyloxybenzoic acid (8), caffeic acid (9), gambirine (10), gambireine (11),1,1-dimetheyl-2-acetl-diethyl ether (12), respectively. All compounds were isolated from this genus for the first time.

Alkaloids↗
PubMed2006

[Comparison between PET/CT and MRI in diagnosing lymph node metastasis and N staging of nasopharyngeal carcinoma].

OBJECTIVE: To compare the clinical significance of PET/CT and MRI in diagnosing lymph node metastasis and N staging of nasopharyngeal carcinoma (NPC). METHODS: 116 NPC patients had undergone PET/CT and MRI before therapy. The findings of PET/CT and MRI in diagnosing lymph node metastasis and N staging were compared according to the results of follow-up. RESULTS: A total of 614 lymph nodes in 116 patients were analyzed. 340 positive nodes and 274 negative nodes interpreted by image findings were verified during follow-up. The sensitivity, specificity and accuracy of PET/CT in diagnosing node metastasis was 93.2%, 98.2% and 95.4%, while that of MRI was 88.8%, 91.2% and 89.9%, respectively, with statistically significant difference in each between PET/CT and MRI (P < 0.05). Based on Fuzhou Staging System, 109 patients (94.0%) were correctly staged by PET/CT, and 103 patients (88.8%) by MRI, while according to UICC Staging System, 108 patients (93.1%) were correctly staged by PET/CT, and 100 patients (86.2%) by MRI. CONCLUSION: PET/CT is superior to MRI in diagnosing lymph node metastasis and N staging of nasopharyngeal carcinoma. The false-positive and false-negative assessment based on PET-CT scan findings may be caused by: (1) inflammatory hyperplastic node; (2) node with large areas of necrosis; (3) node in diameter less than spatial resolution limitation of PET.

Adolescent↗
PubMed2006

[Clinical study on effect of ganneng dripping pill in treating chronic hepatitis B].

OBJECTIVE: To observe the antiviral efficacy of Ganneng Dripping Pill (GDP) in treating patients with chronic hepatitis B. METHODS: Eighty-four patients of chronic hepatitis B with positive HBV-DNA, HBsAg, and HBeAg were randomly assigned to 2 groups, the treated group (56 patients) treated with GDP and the control group (28 patients) treated with lamivudine. The therapeutic course for both groups was 6 months. The negative conversion rate of HBeAg and HBV-DNA, changes of liver function, symptoms and physical signs in patients were observed before and after treatment. RESULTS: In the treated group, the negative conversion rate of HBV-DNA and HBeAg was 54.0% (27/50) and 30.0% (15/50) respectively, and the ALT normalizing rate was 80.0% (40/50), while those were 67.86% (19/28), 21.8% (6/28) and 75.0% (21/28) respectively in the control group, showing no significant difference between the two groups (all P > 0.05). However, the AST normalizing rate was 97.96% (48/49) in the treated group, higher than that in the control group (48.0%, 12/25, P < 0.01); and the clinical symptoms, especially the poor appetite, lassitude, gastric distention and yellow urine, were improved more significantly in the treated group than those in the control group (P <0.05 or P < 0.01). CONCLUSION: GDP has a favorable short-term effect on chronic hepatitis B, it could effectively inhibit HBV replication and improve the liver function, symptoms and signs in patients.

Adult↗
PubMed2006

[Immunophenotypic and cytogenetic features of acute myelomonocytic leukemia].

BACKGROUND & OBJECTIVE: The expression of some antigens has been involved in cytogenetic abnormalities in leukemia. This study was to explore the immunophenotypes of acute myelomonocytic leukemia (M4), analyze the correlation of M4 to cytogenetic abnormalities, and provide evidences for the diagnosis and therapy. METHODS: Immunophenotypic analysis was performed using a panel of monoclonal antibodies and three-color immunofluorescent staining methods of flow cytometry in 81 patients with M4 leukemia. Cytogenetic records of 73 patients were available, among which 35 were further investigated by dual-color interphase fluorescent in situ hybridization (FISH) and the immunophenotype of inv(16)-positive patients were analyzed. RESULTS: Among the 81 M4 leukemia patients, CD33 (84.0%) was the most commonly expressed antigen, followed by CD13 (81.5%) and CD14 (24.7%). CD34 was detected in 48 (59.3%) patients. T lymphoid-and B lymphoid-associated antigens were expressed in 23 (28.4%) and 10 (12.3%) patients, respectively. All the 11 patients with M4Eo had CD13 expression. CD33, CD34, and CD2 were expressed in 10, 7, and 5 patients, respectively. Of the 14 patients with inv(16), 13 expressed CD13, 11 expressed CD33, 8 expressed CD34, 5 expressed CD14, 3 expressed CD7, and 3 expressed CD2. Of the 73 patients with cytogenetic records, 30 (41.1%) had clonal chromosomal abnormalities. The expression of CD2 and CD34 was associated with karyotypic abnormalities. The expression of CD2 was higher in M4Eo patients, with no correlation to inv(16). CONCLUSIONS: Acute myelomonocytic leukemia mainly expresses myeloid lineage antigens. The expression of CD2 and CD34 is correlated to karyotypic abnormalities. The expression of CD2 is higher in M4Eo. Inv(16) exists in 40% of M4 patients and the expression of all the antigens has no correlation to inv(16).

Adolescent↗
PubMed2006

[Identification of serum biomarkers for ovarian cancer using protein chips and time of flight mass spectrometry technology].

OBJECTIVE: To screen relatively specific biomarkers in serum of ovarian cancer patients using surface-enhanced laser desorption and ionization time of flight mass spectrometry and protein chip technology. METHODS: Serum samples from 99 ovarian cancer patients, 87 healthy volunteers and 21 patients with other ovarian diseases were analyzed using metal affinity chromatography protein chip IMAC3 and cation exchange protein chip WCX2, which can specifically bind the metal-combining-proteins. Proteomic spectra were generated by mass spectrometry. The peaks were detected and filtrated by Ciphergen proteinchip software 3.2.0. Using the Biomarker Pattern 5.0 software, a diagnostic system was developed for separating ovarian cancer from the healthy group. RESULTS: Thirty-one protein peaks were significantly different between ovarian cancer patients and healthy controls (P < 0.05). A diagnostic model consisting of protein peaks from IMAC3 and WCX2 was established. Large scale blind test generated a sensitivity of 100% and specificity of 98% respectively. A differentially expressed protein with a mass-to-charge rate of 7769 was identified as potential biomarkers to distinguish ovarian cancer from other ovarian diseases. CONCLUSIONS: Time of flight mass spectrometry is a useful tool for the detection and identification of new protein markers in serum. It will provide a highly accurate and innovative approach for the diagnosis of ovarian cancer.

Adult↗
PubMed2006

[Rapid detection of influenza virus A by fluorescence quantitative RT-PCR].

OBJECTIVE: To develop a method to rapidly detect influenza virus type A by fluorescence real-time quantitative RT-PCR. METHODS: According to conservative sequence of influenza virus type A M2 gene, a pair primers and Taqman probe were designed, respectively. After the quantitative curve of the assay were established using tenfold serial dilution of TCID50, the sensitivity and the specificity of clinic samples were determined. RESULTS: The detection sensitivity of the assay was 2.56 x 10(-5) TCID50 and the regression coefficient of the quantitative curve was 0.999. The specificity was determined by testing five other specimens, all of which yielded negative results. CONCLUSION: The detection system based on real-time RT-PCR was rapid, sensitive and steady, which could be used to detect the clinic samples.

Fluorescence↗
PubMed2006

[Mediastinal lymph node staging in non-small cell lung cancer: comparison between spiral computed tomographic findings and pathology].

BACKGROUND & OBJECTIVE: Mediastinal lymph node staging in non-small cell lung cancer (NSCLC) is important to choose standard treatment plan and estimating prognosis. This study was to evaluate the clinical value of spiral CT in staging mediastinal lymph node in NSCLC through comparing spiral CT findings with corresponding pathology. METHODS: A total of 89 patients with pathologically proven NSCLC received spiral CT and mediastinoscopic biopsy. The spiral CT findings and corresponding pathologic findings in staging mediastinal lymph node were compared. The sensitivity, specificity, and accuracy of diagnosing mediastinal lymph node metastasis were calculated. RESULTS: Compared with corresponding pathologic results, the overall sensitivity, specificity, positive predictive value, negative predictive value, and accuracy of mediastinal lymph node staging with spiral CT were 58.9%, 70.0%, 60.5%, 68.6%, and 65.2%, respectively. The accuracy and specificity of spiral CT was relatively low in staging 4R, 5, 6, 7 lymph node groups; the false negative and false positive rates were relatively high in staging 4R, 7 lymph node groups. CONCLUSIONS: Spiral CT is a non-invasive clinical examination which may be used in staging mediastinal lymph node in NSCLC; however, there exists false negative and false positive rates. It should be combined with other investigations, such as mediastinoscopy, to assess a more accurate mediastinal lymph node staging.

Adult↗
PubMed2006

[The research on preparing CeO2 nanocrystalline by homogeneous precipitation method].

CeO2 nanocrystallines were prepared by homogeneous complexed-precipitation method, using cerous nitrate and ammonium tartrate as raw materials. The effects of cerous tartrate complex compound and the way of producing precipitation on the particle size of samples were investigated. The samples were characterized by XRD, TEM and SEM. The SEM micrograph shows that the foam exhibits a perforated porousness stereostructure in shape, and the HRTEM picture of the particles reveals the clear crystal lattice. All the results indicate that the samples were CeO2 nanocrystalline.

Cerium↗