Biomedical subjects

Li Luo

Publications and source records attributed to Li Luo.

31 records · Page 2Linked to original sources

PubMed2005

[Implementation analysis of public functions of centers of disease prevention and control of China].

OBJECTIVE: To evaluate the implementation of public function and items of the centers of disease prevention and control(CDC). METHODS: 161 centers of disease prevention and control have been investigated by two-stage stratified sampling. The implementation analysis of public function and items of CDC is measured by multiplying average operational proportion and average operational degree. RESULTS: 1. For investigated centers, the average rate of function implementation is 42.9% ,with 56.0% at provincial level and 43.7% at city level, 41.3% for the county level; while 49.3% in east areas, 45.4% in middle areas and 35.3% in west areas. 2. Among all 7 functional items, the implemented rate of disease prevention and control is 54.3%, 65.8% for emergency treatment, 35.0% for epidemic situation report and information management of health relative factors, 31.3% for inspection and control of health risk factors, 39.1% for the laboratory examination and evaluation, 36.4% for health education and health promotion, and 56.7% for technological guidance and application study. CONCLUSION: The implemented degree of public function of CDC was generally lower, varied with regions and levels. Among 7 public functional items, the emergency treatment function had the highest implementation, with the lowest for the inspection and control of health relative factors.

China↗

PubMed2005

[Operational proportion of the public function and items of 161 centers of disease prevention and control in 7 provinces].

OBJECTIVE: To explicate the operational proportion of public function and items of the centers of disease prevention and control (CDC). METHODS: 161 CDC have been investigated by two-stage stratified sampling to provide the information about the operation of the public function and items of CDC. RESULTS: (1) The average operational proportion investigated CDC is 71.7%, the province level 92.0%, the city level 73.7%, the county level 68.5%. East areas 76.5%, middle areas 71.5%, west areas 68.1%. (2) The operational proportion of disease prevention and control function and emergency treatment function were higher, but that of epidemic situation report and information management of health relative factor, inspection and control of health relative factor, and the laboratory examination and evaluation were lower. CONCLUSION: Public function and items were rather poorly carried out by the centers of prevention and control in China, especially those in middle and west areas, and those in counties.

China↗

PubMed2005

[The effects of Mcl-1 gene on ATRA-resistant HL-60 cell].

OBJECTIVE: To investigate the role of Mcl-1 gene in resistance of all-trans retinoic acid (ATRA) of leukemia cells. METHODS: Long-term, intermittent and repetitive exposure of HL-60 cells to ATRA was used to establish a multidrug-resistance cell line (HL-60/ATRA). HL-60/ATRA cells were transfected with Mcl-1 small interference RNA (siRNA) by Lipofectamine 2000. Western blot was used to detect the expression of Mcl-1. The proliferation, apoptosis and differentiation were evaluated by MTT assay, in situ nick end-labeling (TUNEL) and NBT assay, respectively. RESULTS: The HL-60/ATRA could keep its undifferentiated and proliferative status to a high concentration of ATRA (100 nmol/L) with highly expressed Mcl-1 protein (relative grey scale 0.624 +/- 0.127). Mcl-1 gene knockdown by siRNA (relative grey scale 0.267 +/- 0.086) could reverse the resistance of ATRA of HL-60/ATRA by inhibiting proliferation, and inducing differentiation and apoptosis [apoptosis rate (18.5 +/- 4.5)%]. CONCLUSION: Mcl-1 gene might be involved in ATRA resistance in HL-60 cells and inhibiting its expression could be a new approach to ATRA resistance reversion.

Apoptosis↗

PubMed2005

[Implementation analysis of public functions of 161 centers of disease prevention and control in 7 provinces].

OBJECTIVE: To evaluate the implementation of public function and items of the centers of disease prevention and control (CDC). METHODS: 161 centers of disease prevention and control have been investigated by two-stage stratified sampling. The implementation analysis of public function and items of CDC is measured by multiplying average operational proportion and average operational degree. RESULTS: (1) For investigated centers, the average rate of function implementation is 42.9%, with 56.0% at provincial level and 43.7% at city level, 41.3% for the county level, while 49.3% in east areas, 45.4% in middle areas and 35.3% in west areas. (2) Among all 7 functional items, the implemented rate of disease prevention and control is 54.3%, 65.8% for emergency treatment, 35.0% for epidemic situation report and information management of health relative factors, 31.3% for inspection and control of health risk factors, 39.1% for the laboratory examination and evaluation, 36.4% for health education and health promotion, and 56.7% for technological guidance and application study. CONCLUSION: The implemented degree of public function of CDC was generally lower, varied with regions and levels. Among 7 public functional items, the emergency treatment function had the highest implementation, with the lowest for the inspection and control of health relative factors.

China↗

PubMed2005

[Human resources allocation in centers of disease prevention and control in China].

OBJECTIVE: To compare the human resources allocation of CDC in different areas and different levels. METHODS: By stratified cluster sampling we get 70 CDC's manpower data, which come from different areas(east, middle part, west)and different levels (province, city, county). RESULTS: (1) The average number of CDC's staff is 62. (2)CDC of province level are understaffed by 15%, while CDC of county level are overstaffed by 3%, and CDC of county level are overstaffed by 12%. (3) Structure of age: The average age of CDC's staff is 62, people under 35 years old in CDC is 39.4%, people above 55 years old is 3.5% (4) Structure of education: People with graduate degree are only 0.8%, and people come from technicalsecondary school are 57.0%. (5) Structure of specialty: A majority of people's majors are clinical medicine, and the percent of people having a non-medical-major are 36.6%. (6) Area distributing. Distribution of average quality score of staff are imbalance in different areas, the average quality score of staff in east area are 1.07 times than that in middle area, and 1.12 times than that in west area.

China↗

PubMed2005

[Factors and indicators which should be considered in calculating human resources allocation criteria of the centers of disease prevention and control in China].

OBJECTIVE: To develop a series of factors and indicators which should be considered in calculating human resources allocation criteria of the centers of disease prevention and control in China. METHODS: Based on the measurement methodology on CDCs' human resources rational allocation, which has developed by the research team, the paper makes the method of experts' consultation and the datum on whether 168 simple CDCs agreed with these influencing factors and indicators. RESULTS: The paper develops 11 factors and 23 indicators, and finds these factors and indicators have a high agreement in simple CDCs. CONCLUSION: These factors and indicators are scientific, acceptable and reasonable.

China↗

PubMed2005

[Study on insufficiency of government investment to the centers of disease prevention and control].

OBJECTIVE: To make clear the superaddition of government investment to the centers of disease prevention and control (CDC) which will ensure fulfillment of public functions of the center of disease prevention and control. Methods Centers of disease prevention and control had been sampled by two-stage stratified sampling method to investigate the status of government investment to the centers of disease prevention and control, which would be compared with the expected government investment to fulfill the public functions of CDC. RESULTS: According to the cost of manpower and the price in 2003, the government should give another 304.9% investment than current government investment (3.88 billions). For CDC of county level, the superaddition rate even reached 416.1%. The percentage of manpower expense would be 32.2% and the percentage of routine expense would be 67.8% of the superaddtion of government investment. The percentage of routine expense even reached 70.7% of the superaddtion of government investment. CONCLUSION: There was a big gap between the current government investment to CDC and the expected government investment to CDC. To fulfill the public function of CDC, the current government investment to CDC should increase by 3-4 times. The superaddition of government investment to CDC should be allocated more to the routine expense.

China↗

PubMed2005

[Study on the fund to ensure the implementation of public function of province-level, city-level and county-level center of disease prevention and control in China].

OBJECTIVE: To calculate the fund to ensure the implementation of public function of province-level, city-level and county-level center of disease prevention and control in China. METHODS: The principle was to fulfill public function, promote professional efficiency and give a comprehensive attention to employee depletion. Basic data were collected by sample CDC investigation. Value of some special indicators was demonstrated by specialist group. Results To ensure the implementation of public function, a total of 15.7 billion Yen per year should be allocated to all province-level, city-level and county-level center of disease prevention and control. The personnel expenses was 8.4 billion Yen and the daily expenses was 7.4 billion Yen per year.

China↗

PubMed2004

Resolution of anaphase bridges in cancer cells.

Chromosomal instability is a key step in the generation of the cancer cell karyotype. An indicator of unstable chromosomes is the presence of chromatin bridges during anaphase. We examined in detail the fate of anaphase bridges in cultured oral squamous cell carcinoma cells in real-time. Surprisingly, chromosomes in bridges typically resolve by breaking into multiple fragments. Often these fragments give rise to micronuclei (MN) at the end of mitosis. The formation of MN is shown to have important consequences for the cell. We found that MN have incomplete nuclear pore complex (NPC) formation and nuclear import defects and the chromatin within has greatly reduced transcriptional activity. Thus, a major consequence of the presence of anaphase bridges is the regular sequestration of chromatin into genetically inert MN. This represents another source of ongoing genetic instability in cancer cells.

Anaphase↗

PubMed2004

Sinorhizobium meliloti ExoR and ExoS proteins regulate both succinoglycan and flagellum production.

The production of the Sinorhizobium meliloti exopolysaccharide, succinoglycan, is required for the formation of infection threads inside root hairs, a critical step during the nodulation of alfalfa (Medicago sativa) by S. meliloti. Two bacterial mutations, exoR95::Tn5 and exoS96::Tn5, resulted in the overproduction of succinoglycan and a reduction in symbiosis. Systematic analyses of the symbiotic phenotypes of the two mutants demonstrated their reduced efficiency of root hair colonization. In addition, both the exoR95 and exoS96 mutations caused a marked reduction in the biosynthesis of flagella and consequent loss of ability of the cells to swarm and swim. Succinoglycan overproduction did not appear to be the cause of the suppression of flagellum biosynthesis. Further analysis indicated that both the exoR95 and exoS96 mutations affected the expression of the flagellum biosynthesis genes. These findings suggest that both the ExoR protein and the ExoS/ChvI two-component regulatory system are involved in the regulation of both succinoglycan and flagellum biosynthesis. These findings provide new avenues of understanding of the physiological changes S. meliloti cells go through during the early stages of symbiosis and of the signal transduction pathways that mediate such changes.

ADP Ribose Transferases↗

PubMed2003

Design and characterization of a hyperstable p16INK4a that restores Cdk4 binding activity when combined with oncogenic mutations.

Cyclin-dependent kinase inhibitor p16(INK4a) is the founding member of the INK4 family of tumor suppressors capable of arresting mammalian cell division. Missense mutations in the p16(INK4a) gene (INK4a/CDKN2A/MTS1) are strongly linked to several types of human cancer. These mutations are evenly distributed throughout this small, ankyrin repeat protein and the majority of them disrupt the native secondary and/or tertiary structure, leading to protein unfolding, aggregation and loss of function. We report here the use of multiple stabilizing substitutions to increase the stability of p16(INK4a) and furthermore, to restore Cdk4 binding activity of several defective, cancer-related mutant proteins. Stabilizing substitutions were predicted using four different techniques. The three most effective substitutions were combined to create a hyperstable p16(INK4a) variant that is 1.4 kcal/mol more stable than wild-type. This engineered construct is monomeric in solution with wild-type-like secondary and tertiary structure and cyclin-dependent kinase 4 binding activity. Interestingly, these hyperstable substitutions, when combined with oncogenic mutations R24P, P81L or V126D, can significantly restore Cdk4 binding activity, despite the divergent features of each destabilizing mutation. Extensive biophysical studies indicate that the hyperstable substitutions enhance the binding activity of mutant p16 through several different mechanisms, including an increased amount of secondary structure and thermostability, reduction in exposed hydrophobic surface(s) and/or a reduced tendency to aggregate. This apparent global suppressor effect suggests that increasing the thermodynamic stability of p16 can be used as a general strategy to restore the biological activity to defective mutants of this important tumor suppressor protein.

Amino Acid Sequence↗

PubMed2002

Syntheses, crystal structures, and physical properties of dinuclear copper(I) and tetranuclear mixed-valence copper(I,II) complexes with hydroxylated bipyridyl-like ligands.

Four copper complexes with hydroxylated bipyridyl-like ligands, namely [Cu(2)(ophen)(2)] (1), [Cu(4)(ophen)(4)(tp)] (2), [Cu(4)(obpy)(4)(tp)] (3), and [Cu(4)(obpy)(4)(dpdc)].2H(2)O (4), (Hophen=2-hydroxy-1,10-phenanthroline, Hobpy=6-hydroxy-2,2'-bipyridine, tp=terephthalate, dpdc=diphenyl-4,4'-dicarboxylate) have been synthesized hydrothermally. X-ray single-crystal structural analyses of these complexes reveal that 1,10-phenanthroline (phen) or 2,2'-bipyridine (bpy) ligands are hydroxylated into ophen or obpy during the reaction, which provides structural evidence for the long-time argued Gillard mechanism. The dinuclear copper(I) complex 1 has three supramolecular isomers in the solid state, in which short copper-copper distances (2.66-2.68 A) indicate weak metal-metal bonding interactions. Each of the mixed-valence copper(i,ii) complexes 2-4 consists of a pair of [Cu(2)(ophen)(2)](+) or [Cu(2)(obpy)(2)](+) fragments bridged by a dicarboxylate ligand into a neutral tetranuclear dumbbell structure. Dinuclear 1 is an intermediate in the formation of 2 and can be converted into 2 in the presence of additional copper(II) salt and tp ligands under hydrothermal conditions. In addition to the ophen-centered pi-->pi* excited-state emission, 1 shows strong emissions at ambient temperature, which may be tentatively assigned as an admixture of copper-centered d-->s,p and MLCT excited states.

Journal Article↗

PubMed2002

Sixty-one cases of angina pectoris due to coronary heart disease treated by external use of the paste of nitrum and realgar powder on zhiyang (GV 9).

In order to inquire into the therapeutic effects of Xiao Shi Xiong Huang San (the Nitrum and Realgar Powder), one of the Dunhuang Prescription, on angina pectoris due to coronary heart disease (APCHD), the authors have treated 61 cases of APCHD by externally applying paste of the powder on Zhiyang (GV 9), with another 30 cases of APCHD treated with the nitroglycerin paste on Zhiyang as the controls. The results showed that the total effective rate was 82% and markedly effective rate 31.2% in the treatment group (the Paste of Nitrum and Realgar Powder), and the total effective rate was 46.6% and markedly effective rate 23.2% in the control group (the nitroglycerin paste). The difference in therapeutic effects between the two groups was very significant (P<0.01), indicating that the therapeutic effect of the former was significantly superior to that of the latter.

Administration, Cutaneous↗