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Biomedical subjects

Li Yong

Publications and source records attributed to Li Yong.

7 recordsLinked to original sources
PubMed2006

Quantitative analysis of trace chromium in blood samples. Combination of the advanced oxidation process with catalytic adsorptive stripping voltammetry.

A new method for pretreating blood samples for trace Cr analysis is described. The advanced oxidation process (AOP with H2O2 and 5.5-W UV irradiation for 60 min) is used to remove biological/organic species for subsequent analysis. Prior to the AOP pretreatment, acid (HNO3) is used at pH 3.0 to inhibit the enzyme catalase in the blood samples. Catalytic adsorptive stripping voltammetry at a bismuth film electrode gives a Cr concentration of 6.0 +/- 0.3 ppb in the blood samples. This concentration was confirmed by dry-ashing the blood samples and subsequent analysis by atomic absorption spectroscopy. This current method may be used to monitor chromium, a trace metal in humans, and the efficacy and safety of chromium supplements as adjuvant therapy for diabetes.

Adsorption↗
PubMed2006

Syntheses and structures of sterically congested linear and branched cobalta[n]triangulanes.

Treatment of {eta(5):eta(1)[2-(di-tert-butylphosphanyl-P)ethyl]cyclopentadienyl}cobalt(I) chloride (5) with methylenecyclopropane (3) or bicyclopropylidene (4), as well as with their spirocyclopropanated analogues methylenespiropentane (7), cyclopropylidenespiropentane (10), or 7,7'-bi(dispiro[2.0.2.1]heptylidene) (15) in the presence of sodium amalgam at -50 degrees C, furnished the stable cobalt complexes 6, 9, 8, 11, and 16, respectively, in 72, 83, 84, 86, and 54 % isolated yield, respectively. The complexes 14 and 16 were also obtained by ligand exchange of the ethene complex {eta(5):eta(1)[2-(di-tert-butylphosphanyl-P)ethyl]cyclopentadienyl}(eta(2)-ethene)cobalt(I) (12) with 13 and 15 in 79 and 52 % yield, respectively. The X-ray crystal-structure analyses of complexes 9, 14, and 16, as well as the NMR-spectroscopic data of all complexes, reveal that they can be regarded as linear and branched cobalta[n]triangulanes. The thermal stability of complexes 6, 8, and 9 up to 109, 145, and 160 degrees C was determined by differential thermal analysis-thermogravimetry (DTA-TG) analysis.

Journal Article↗
PubMed2005

Chinese soft-shelled turtle egg powder lowers serum cholesterol, increases faecal neutral steroids and bile acid excretion, and up-regulates liver cytochrome P450 mRNA level in rats.

The aim of the present study was to investigate the effect of Chinese soft-shelled turtle whole egg powder (TE) on cholesterol metabolism in Sprague-Dawley rats to determine whether it has a cholesterol-lowering effect. Forty male Sprague-Dawley rats were fed a high-fat diet supplemented with TE (0, 0.75, 1.50 or 3.00 g/kg body weight) administrated by gavage for 24 weeks. Serum total cholesterol (TC), HDL-cholesterol (HDL-C), LDL-cholesterol (LDL-C) and faecal total bile acids levels were determined by enzymatic methods. Faecal steroid concentrations were measured by GC. Means and standard deviations were calculated where appropriate for values, and the data were tested by one-way ANOVA. After 24 weeks of feeding a high-fat diet with TE supplementation, serum TC and LDL-C levels, liver cholesterol and liver lipid levels were reduced in rats. TE supplementation did not affect the faecal output, but significantly increased steroid concentrations in faeces, indicating increased steroids excretion. The faecal bile acid excretion was also increased as evidence by elevated mRNA level of liver cytochrome P450, family 7, subfamily A, polypeptide 1. Our results demonstrated that the TE does have a cholesterol-lowering effect by increasing the excretion of total bile acids and neutral steroids.

Animals↗
PubMed2005

[Rapid analysis of genetically modified soybean by a duplex PCR-capillary electrophoresis system with laser-induced fluorescence detection].

OBJECTIVE: To develop a rapid detection method for genetically modified soybean resistant to glyphosate. METHODS: A duplex PCR was performed with primers designed in this study to simultaneously amplify heterogenous genes in transgenic soybean: CaMV-35S promoter, NOS terminator and CP4-EPSPS gene. And a simple capillary electrophoresis with laser-induced fluorescence detection (CE-LIF) was developed and applied to the rapid analysis of the above PCR products by using a 50 cm length x 100 microm i.d. capillary coated with linear polyacrylamide and an 8 g/L HPMC-4000 sieving buffer under 200 V/cm electric field strength. RESULTS: The proposed method was able to simultaneously detect the three heterogenous genes existing in genetically modified soybean under the optimization conditions of PCR and capillary electrophoresis. The measured sequences of the duplex PCR products were identical with the original genes' sequences. Moreover, the sample volumes required were not more than 5 nl and the detection could be completed in less than 24 min. The relative standard deviations (R. S. D.) of the migration times for the PCR products were < or = 3.2%. CONCLUSION: In comparison with agarose gels electrophoresis, the duplex PCR-based capillary electrophoretic method with laser-induced fluorescence detection is rapid, sensitive and accurate, and it is suitable for detection of genetically modified soybean.

DNA, Plant↗
PubMed2003

External quality assessment of automated hematology analyzer performance using fresh human blood samples in Shanghai.

We set out to assess the performance and degree of agreement for the different automated hematology analyzers (AHAs) currently used within the Shanghai region. Analyzers from 5 different manufacturers were represented within the 114 hospitals that participated in this project: Abbott, ABX, Beckman Coulter, Nihon Kohden, and Sysmex. We determined the results obtained on 115 hematology analyzers in the 114 different hospitals for hemoglobin (HGB), red blood cell count (RBC), white blood cell count (WBC), hematocrit (HCT), and platelet count (PLT) from fresh anticoagulated blood samples from healthy donors. The maximum coefficients of variation (CV%) among instruments of the 3 main manufacturers (Abbott, Beckman Coulter, and Sysmex) for RBC, HGB, HCT, WBC, and PLT were 3.2%, 3.8%, 3.6%, 9.3%, and 10.8%, respectively. The maximum deviations observed among these parameters from the 5 major instrument groups were 0.74% (RBC), 2.24% (HGB), 6.37% (HCT), 6.97% (WBC), and 21.06% (PLT). Implementation of the regular, local external quality assessment program in the Shanghai region using fresh blood samples has resulted in a reduction of CV% values for the major clinically important CBC parameters (RBC, HGB, HCT, WBC, and PLT) within the instrument groups over time. An important result was that the CBC parameter CVs were also well within established US Clinical Laboratory Improvement Amendments of 1988 guidelines for AHA performance. Combined with a comprehensive education program, the Shanghai external fresh blood quality assessment program has resulted in closer agreement among the various AHA manufacturers, with only very small differences for RBC, HGB, HCT, WBC, and PLT counts. The success of this program has given us increased confidence in the comparability of results and subsequent patient management using the various types of AHAs currently in use in the hospitals within the Shanghai region.

Automation↗