[Analysis of non-improving symptoms after endoscopic sinus surgery for chronic sinusitis].
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Adult↗
Biomedical subjects
Li-min Luo
Publications and source records attributed to Li-min Luo.
[A review of methods for correction of artifacts in functional MRI].
There are many kinds of artifacts in image series of functional MRI, such as head motion artifacts, physiological motion artifacts, blood flow artifacts, ghost artifacts and susceptibility artifacts. These artifacts which are unassociated with the neural activities, have so severe affects on the analysis of functional MRI data that they not only reduce the sensibility and reliability of functional MRI, but also make the detecting, locating and visualizing of the functional active regions more complicated. The mechanism and the effect of artifacts in functional MRI are discussed here, the methods of correcting artifacts are reviewed, and research prospects are discussed too.
Artifacts↗
[Immunogenicity of a multiple epitope DNA vaccine against hepatitis B virus].
AIM: To study the immunogenicity of a multiple epitope DNA vaccine against hepatitis B virus(HBV). METHODS: A multiple epitope HBV antigen gene BPT was synthesized and cloned into eukaryotic expression vector pcDNA3.1 and then BALB/c mice were immunized with the DNA vaccine. The specific humoral and cellular immune responses were detected by indirect ELISA, cytotoxicity of CTL, and lymphocyte proliferation. The immunized mice were also observed for the possible toxicity and side effects after administration of the DNA vaccine. RESULTS: Immunization with pcDNA3.1/BPT elicited high-level antigen-specific IgG and antigen-specific CTL response, and stimulated lymphocyte proliferation. RT-PCR analysis of spleen lymphocytes showed that levels of IL-12 mRNA in immunized mice were notably higher than that in control mice. CONCLUSION: The multiple epitope HBV DNA vaccine can induce specific humoral and cellular immune responses, which lays a certain foundation for development of prophylactic and therapeutic HBV vaccine.
Animals↗
[Inhibitory effect of recombinant human endostatin on angiogenesis and lung metastasis of mouse lung adenocarcinoma LA795].
OBJECTIVE: To study the inhibitory effect of recombinant human endostatin (rhES) on the angiogenesis and lung metastasis of mouse lung adenocarcinoma LA795. METHODS: The recombinant yeast strain containing the gene sequence encoding highly soluble rhES was induced by methanol for rhES production, which was purified with heparin affinity chromatography. T739 mice with subcutaneous inoculation of LA795 cells were randomized into 2 groups (10 in each group) to receive injection of either rhES (20 mg/kg x b x w x per day) or PBS in the same volume for 14 consecutive days starting from the sixth day after the inoculation. The angiogenesis and lung metastasis of the implanted tumors were subsequently observed. RESULTS: Purified rhES was successfully obtained. As shown by immunohistochemistry, the tumors in the mice receiving rhES treatment exhibited less density of the microvessels than those in the PBS-treated mice did (P<0.01). Pathological examination of the lung tissue of the mice in rhES group found no visible signs of tumor metastasis, which, in contrast, was widespread in PBS group. The weight of the lungs was also significantly different (P<0.01). CONCLUSION: rhES possesses good biological properties and can potently inhibit the angiogenesis and lung metastasis of mouse lung adenocarcinoma LA795.
Adenocarcinoma↗
[Synthesis, cloning, expression and antigenicity of the therapeutic multi-epitope gene of hepatitis B virus and of its products].
OBJECTIVE: To study the synthesis, cloning, expression and antigenicity of therapeutic multi-epitope gene of hepatitis B virus. METHODS: The therapeutic multi-epitope gene of hepatitis B virus was synthesized and cloned into the vector pWR450-1, then was expressed in E.coli and the products were purified. The immunogenicity of the expressed protein was analyzed by Western-blotting. RESULTS: Recombinant plasmid PWR/BPT was constructed successfully and the protein of multi-epitope gene of hepatitis B virus was expressed in E. coli, which showed ideal antigenicity by Western-blotting. CONCLUSIONS: The designed multi-epitope therapeutic gene of hepatitis B virus was proved to be correct and the expressed protein may be a good therapeutic vaccine.
Blotting, Western↗
[Inhibitory effects of recombinant human endostatin on growth and metastasis of lung adenocarcinoma LA795 in mice].
BACKGROUND & OBJECTIVE: Generally, growth and metastasis of tumor are critically dependent on angiogenesis. Endostatin can specifically inhibits tumor angiogenesis. The current study was designed to evaluate the inhibitory effect of recombinant human endostatin (rhES) secreted by pichia, pastoris, GS115 on the growth and metastasis of mice lung adenocarcinoma LA795 in mice T739. METHODS: To select a strain that could highly express recombinant human endostatin, then induce the clone to express rhES by adding methanol. Purification of rhES was performed with heparin affinity chromatography. LA795 tumor cells were inoculated subcutaneously into the dorsa of T739 mice, and the mice were randomized into two groups. The first group was given rhES(20 mg/kg/d), and the second group was given equal volume of PBS, for 14 consecutive days. The volume of tumors were measured. And the tumor metastasis in the lungs of the mice was observed. RESULTS: The selected clone was induced to secrete enough soluble rhES. The purified protein could strongly inhibit growth and metastasis of mice lung adenocarcinoma LA795 in T739 mice (P < 0.001). CONCLUSION: The rhES secreted by pichia, pastoris, GS115 has good biological activities and greatly inhibit growth and metastasis of mice lung adenocarcinoma LA795 in mice T739.
Adenocarcinoma↗
[Virtual imagery in medicine].
The development of modern medical imaging and related technologies, and the increasing requirements for accuracy in clinical diagnosis and treatments give the emergence of a new discipline--virtual imagery in medicine. In this paper, we try to summarize its technical aspects, including virtual reality, 3-D image reconstruction and visualization, virtual endoscopy, and so on.
Algorithms↗
[The 3D PACS image system based on pipeline framework].
We put forward an image rendering system based on pipeline framework for processing and displaying medical images. Compared to original computer graphics algorithms divided into volume rendering and surface rendering, this framework can effectively comprehend methods of computer graphics and image processing, import some new concepts such as vertex buffer, pixel buffer and texture buffer. We implement Shaded Surface Display, Maximum Intensity Projection, Digitally Reconstructed Radiography, Multi planar Reformation, Curved Planar Reformation and Interactive Virtual Endoscopy in our new developed PACS image system.
Algorithms↗
[DICOM in radiotherapy].
Digital Image Communication in Medicine (DICOM) defines a standard method to store and transmit digital medical image information, in which there is a piece of implemented protocol named DICOM-RT that specially addresses both the transmission of radiation therapy image data and the ancillary data. In this paper, we firstly introduce the DICOM-RT with the emphases on its components, relationship with radiotherapy and how to produce the DICOM-RT object that refer to some certain radiotherapy information. Then we expatiate on the impact that benefits from applying DICOM-RT to radiotherapy, with an aid to accelerate its application in China.
Computer Communication Networks↗