Biomedical subjects

Li-ming Zhang

Publications and source records attributed to Li-ming Zhang.

10 recordsLinked to original sources

PubMed2006

[Effect of local mild hypothermia on expression of aquaporin-4 following intracerebral hemorrhage in rats].

OBJECTIVE: To examined the effect of local mild hypothermia on the expression of aquaporin-4 (AQP-4) following intracerebral hemorrhage (ICH) in rats and clarified the mechanism of hypothermia on brain edema formation following ICH. METHODS: Two hundreds and forty male Wistar rats were randomly divided into two groups: the intracerebral hemorrhage (ICH) group, in which autologous arterial blood were stereotaxically injected into right caudate nucleus; the local mild hypothermia (ICH + H) group, in which the rats were given 4 h local mild hypothermia after the injection of blood. Each group was divided into 6 subgroups: control, 6 h, 24 h, 72 h, 5 d and 7 d after operation; Brain water content was determined by dry-wet weight method and the permeability of BBB was measured by Evans-Blue extravasation. RT-PCR and Western blot were respectively used to evaluate AQP-4 mRNA and protein expression. RESULTS: In ICH group, compared with control, ICH significantly increased BWC, the permeability of BBB and the expression of AQP-4 mRNA, all began at 6 h and peaked at 72 h (P < 0.01), the increased protein expression of AQP-4 began at 24 h and also peaked at 72 h (P < 0.01). AQP-4 expression positively correlated, both at the mRNA and the protein level, with the permeability of BBB (r = 0.78 and r = 0.76 respectively). In ICH + H group, compared with ICH group, the elevation of BWC, BBB permeability and AQP-4 protein expression were strongly attenuated at all time point by hypothermia treatment (P < 0.01), while AQP-4 mRNA levels demonstrated a modest attenuation from 48 h. At 72 h, AQP-4 mRNA optical density (A) decreased from 1.25 +/- 0.03 (ICH group) to 1.04 +/- 0.02 (P < 0.01), AQP-4 protein expression (A) decreased from 0.77 +/- 0.08 (ICH group) to 0.25 +/- 0.04 (P < 0.01). CONCLUSIONS: This study indicates that BBB breakdown can increase the expression of AQP-4; local mild hypothermia can significantly reduce brain edema formation after ICH by suppressing the elevation of AQP-4 protein expression; Inhibition of BBB breakdown and the elevation of AQP-4 protein expression with local mild hypothermia appear to contribute to brain protection in this model.

Animals↗

PubMed2006

[The calibration of instrument response function during passive FTIR measurement].

In order to obtain infrared spectral radiance distribution of some infrared sources, such as spectral radiant flux density, spectral radiant intensity, spectral radiance and spectral irradiance, the instrument response function under different conditions must be known. In the present paper, the calibration of instrument response function during passive FTIR measurement has been discussed. The experimental results illustrate that under different experimental conditions, the instrument response function varies not only with the temperature of the blackbody but also with the signal amplitude received by the infrared instrument. So, during passive FTIR measurement, the temperature and the emission signal amplitude of the source must be observed carefully in order to get satisfactory instrument response function.

English Abstract↗

PubMed2006

[Automated recognition of VOCs using artificial neural networks].

Quantitative analysis of FTIR spectra, which are seriously overlapped in the spectral bands, was studied by artificial neural networks. The optimum network was chosen by a new criterion, i. e. the degree of approximation. After the network was established, two kinds of spectra were resolved. It was demonstrated that accurate results could be obtained when two components were both included. In addition, the unknown spectrum could be identified and quantified. It was showed that the artificial neural network has excellent non-linear ability of solution. Meanwhile, the method provides an efficient approach to the identification and quantification of the unknown samples.

English Abstract↗

PubMed2006

[Remote passive detection of flame temperature of solid propellant adulterating nanoparticles].

The flame temperature of three kinds of solid propellants was measured by passive remote sensing FTIR with the resolution of 1 cm(-1). These three kinds of solid propellants are adulterate nano-scale metal oxide particles, adulterate normal metal oxide particles, and propellant without any adulterations. The main components of the solid propellant are nitrocellulose and nitroglycerin. The metallic oxides, including 6 nm CuO, 56 nm Fe2O3, 16 nm NiO, and correspondingly the normal particles, were adulterated into the solid propellants respectively. The flame temperature was calculated through the fine structure of the emission fundamental band of H2O at 2.75 microm. The results of the flame temperature of the solid propellants adulterating nano-scale CuO, Fe2O3 and NiO are 3089, 3193 and 3183 K, respectively. The temperatures of the three kinds of solid propellants were compared, and it was shown that there is no obvious difference in the flame temperature among the three kinds of solid propellants.

English Abstract↗

PubMed2006

[Multi-component analysis of FTIR spectra of non-linear system using polynomial partial least squares method].

A non-linear algorithm, polynomial PLS was applied to the simultaneous analysis of OP-FTIR spectra of a five-component system whose FTIR spectra were seriously overlapped. The results were compared with the one obtained from PLS. PPLS yielded good performance, especially for the prediction of benzene and chloroform. RMSEP (root mean squared error of prediction) of benzene and chloroform in PPLS model were 0.043 and 0.087 and the corresponding values in PLS were 0.402 and 0.842, respectively. Meanwhile, variance was accounted by PPLS with fewer latent variables, which indicates the simplicity and robustness of the model. The successful application of PPLS to non-linear system was meaningful for the use of remote sensing FTIR in air monitoring.

English Abstract↗

PubMed2006

[Advanced development of remote sensing FTIR in air environment monitoring].

Remote sensing FTIR(RS-FTIR) is one of the most important technologies in atmospheric pollutant monitoring. It has several prevailing advantages: (1) high resolution and high selectivity; (2) it requiring no sampling and no sample preprocessing; (3) capability of detecting several compounds simultaneously; (4) real time, long distance, and automatic monitoring. So it is extraordinarily appropriate for the quantitative and qualitative determination, and remote, real time, dynamic monitoring of air contaminant, especially the toxic volatile organic compounds (VOCs). The present paper reviews the research progress in remote sensing FTIR for air monitoring made by the Laboratory of Advanced Spectroscopy in recent years, including chemometrics, computed tomography, FTIR spectra interpretation, spatial distribution of air pollutant concentration and passive remote monitoring, etc. These research findings show that the rapid development and application of remote sensing FTIR accelerated the temporal and spatial extension of analytical chemistry, and it will by all means have wide prospects in atmospheric environmental monitoring.

English Abstract↗

PubMed2005

[Junctional epithelialization of dental implants in animals].

OBJECTIVE: To detect the histological changes and the expression of proliferating cell nuclear antigen (PCNA) in junctional epithelialization of dental implants. METHODS: The junctional epithelium was detected by HE staining and the PCNA expression was observed by immunohistochemical staining on the st, 3rd, 6th, 9th, 12th, 15th, 20th and 30th day after implantation in Beagle dogs. RESULTS: The junctional epithelium began to form and PCNA was expressed positively 12 days after implantation. The epithelial hyperplasia tended to stabilization and the expression of PCNA weakened drastically 15 days after implantation. The epithelial attachment showed to be similar to that of natural teeth 20 days later. CONCLUSION: The gingiva epithelium can attach to titanium implants forming junctional epithelium which is similar to the natural teeth.

Animals↗

PubMed2005

[Application and improvement of partial-least-squares in Fourier transform infrared spectroscopy].

Partial least squares(PLS) algorithm is an effective chemometric tool. It takes the advantages of multipal linear regression (MLR) and principal component regression (PCR), which makes Fourier transform infrared spectrometry (FTIR) more powerful and useful. Accompanied with increasing use of FTIR, the algorithm is modified and corrected under different circumstances. The applications of PLS to FTIR were mentioned. Improved algorithms were presented, such as moving windows PLS(MWPLS), robust PLS (RPLS), weighted PLS(WPLS), and non-linear PLS. Data pre-processing, selection of variable, noise elimination and non-linear model of PLS were introduced.

English Abstract↗

PubMed2005

[Advances in passive OP-FTIR].

As an OP-FTIR(Open Path FTIR) technology, passive OP-FTIR not only has the advantages of OP-FTIR, but also has the ability to collect data from any direction without prior background information. This technology allows mobile, fast, man-held and stand-off detection of hazardous chemical cloud.The present paper presents some developments of passive OP-FTIR, including high altitude atmospheric pollution detection, auto-detection of toxic cloud, hot gases detection (such as the determination of major combustion products in aircraft exhausts, remote sensing of smoke plumes, and remote sensing of volcano emissions), temperature and combustion products determination (including cloud temperature determination). The present paper also gives the application of passive OP-FTIR in the military. With the development of FTIR and computer science, more and more applications of OP-FTIR to environment supervision, aviation and space flight, engine exhausts, combustion and military will be fulfilled.

English Abstract↗

PubMed2004

[Treatment of Bell's palsy with combination of traditional Chinese medicine and western medicine].

OBJECTIVE: To evaluation the clinical effect of combination of traditional Chinese medicine and western medicine on Bell's palsy. METHODS: 83 patients with Bell's palsy were randomly divided into two groups (trail group 54 cases and control group 29 cases). Patients in two groups were treated with medicine, acupuncture, physiotherapy, while patients in the trail group were treated with massage and functional exercise as the same time. The results of both groups were evaluated according to Portmann's Simple Scale. RESULTS: The score before treatment of trail group was 2.907 +/- 1.794, while control group was 2.931 +/- 2.034. And the score after treatment of trail group was 18.593 +/- 1.743, while control group was 9.862 +/- 3.091. Score of the function of facial muscles obtained from trail group was distinctly higher than that was from the control group (P < 0.01), as well as the improvement index (P < 0.01, trail group: 0.844 +/- 0.095, control group: 0.712 +/- 0.129). CONCLUSION: There is significant curative effect and suitability in the treatment of Bell's palsy with combination of traditional Chinese medicine and western medicine. The improvement of facial muscles' motive function pre- and post-treatment and quantitative evaluation of curative effect can be objectively obtained by evaluation of facial muscles' function.

Acupuncture Therapy↗