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Biomedical subjects

Lifeng Wang

Publications and source records attributed to Lifeng Wang.

10 recordsLinked to original sources
PubMed2026

Age as a core disease modifier: Distinct clinical, molecular and prognostic landscapes of essential thrombocythaemia in adolescents and young adults.

Essential thrombocythaemia (ET) in adolescents and young adults (AYA, 15-39 years) is a distinct entity with an incompletely defined prognosis. In this multicentre retrospective study, 1728 ET patients from 29 centres across China were stratified into AYA (n = 328) and non-AYA (≥40 years, n = 1400) cohorts. We compared their clinical profiles, genomic landscapes, long-term outcomes and risk factors for progression to post-ET myelofibrosis (MF). AYA patients had fewer cardiovascular risks and lower thrombosis rates, but higher rates of extreme thrombocytosis. Molecularly, AYA patients were enriched for calreticulin (CALR) mutations, whereas Janus kinase 2 (JAK2) predominated in older patients. The burden of non-driver mutations (tet methylcytosine dioxygenase 2 [TET2], DNA methyltransferase 3A [DNMT3A], ASXL transcriptional regulator 1 [ASXL1], SH2‑B adaptor protein 3 [SH2B3]) was lower in AYA patients. Consequently, AYA patients achieved superior long-term outcomes across all key survival endpoints, including overall, myelofibrosis-free and leukaemia-free survival. Analysis of post-ET MF progression risks identified age-specific patterns: CALR mutations are enriched in younger patients and show an age-specific association with MF progression. AYA-ET constitutes a unique clinicomolecular subtype with a favourable prognosis, supporting age-stratified management. The enrichment of CALR mutations and their specific link to MF progression in young patients underscore the urgent need for targeted therapies against CALR-mutant clones.

adolescents and young adults (AYA)↗
PubMed2026

Effects of sub-anesthetic doses of esketamine on immune function and postoperative negative emotions in acoustic neuroma patients: a randomized clinical trial.

BACKGROUND: Patients undergoing acoustic neuroma (AN) surgery often experience&#xa0;postoperative negative emotions, including anxiety, depression, and immune function suppression. This trial evaluated whether perioperative sub-anesthetic esketamine improves early postoperative negative emotions and immune function. METHODS: In this single-center, double-blind, randomized trial, 84 patients scheduled for AN surgery were assigned to esketamine (n = 42) or placebo (n = 42). The esketamine cohort received a continuous intravenous infusion of esketamine at 0.2&#x2009;mg&#xb7;kg-1&#xb7;h-1 during anesthesia, followed by 1&#x2009;mg&#xb7;kg-1 esketamine as an adjuvant in patient-controlled intravenous analgesia (PCIA). The placebo group received saline. The primary outcome was the incidence of depression on postoperative day (POD1), defined as a Hospital Anxiety and Depression Scale-Depression subscale (HADS-D) score > 7. RESULTS: Seventy-seven patients completed the study (39 in the esketamine group, 38 in the placebo group). Esketamine significantly reduced the incidence of depression at POD1 (7.7% versus 31.6%; relative risk 0.24, 95% CI: 0.08-0.80, p&#x2009;=&#x2009;0.008) and POD3 (0.0% versus 15.8%, relative risk 0.00, 95% CI: 0.00-0.47, p&#x2009;=&#x2009;0.031) compared with placebo. The incidences of anxiety on POD1 and 3 and sleep disturbances on POD1 were also significantly reduced (p&#x2009;<&#x2009;0.05). Notably, no significant differences were observed between the two groups in terms of immune function, postoperative pain scores, or intraoperative morphine equivalent. Adverse events did not differ between the groups. CONCLUSION: Perioperative sub-anesthetic esketamine reduced postoperative depression and anxiety, and improve sleep quality after AN surgery, without significant effects on early immune function or acute postoperative analgesia. TRIAL REGISTRATION: Chinese Clinical Trial Registry, ChiCTR2400084537.

Humans↗
PubMed2006

Ultralow temperature synthesis of ordered hexagonal smaller supermicroporous silica using semifluorinated surfactants as template.

Ordered hexagonal smaller supermicroporous silica (JLU-14L and JLU-11L) has been successfully synthesized at ultralow temperature (0 approximately -20 degrees C) by semifluorinated surfactants, which is extensively investigated by X-ray diffraction, N2 adsorption, and transmission electron microscopy. X-ray diffraction shows that the peak intensity of the samples increases with decreasing synthesis temperatures in the range of 0 approximately -20 degrees C, indicating that the ordering of porous silica improves with decreasing synthesis temperature. N2 adsorption/ desorption isotherms show that JLU-14L has both ordered smaller hexagonal supermicropores (1.27 and 1.28 nm) and disordered micropores (0.67 nm). Such hierarchically porous materials with micro/supermicroporosity should be potentially important for fast diffusion of reactants and products in catalytic reactions. The ultralow temperature synthesis is a crucial factor for the formation of ordered smaller supermicropores and the control of microporosity in JLU-14L.

Adsorption↗
PubMed2005

Size dependence of the thin-shell model for carbon nanotubes.

There has been much debate on the choice for the representative wall thickness for the thin-shell model, although this model has demonstrated remarkable success in capturing many types of behavior of single-walled carbon nanotubes (SWNTs), in determining the buckling strains under compression, torsion, and bending, in particular. This analysis, using the Tersoff-Brenner potential and ab initio calculations, shows that the elasticity of the model thin shell evolves from isotropic to square symmetric with the decreasing tube diameter, leading to significant diameter dependence for all the elastic moduli and the representative wall thickness. Furthermore, the elastic moduli of multiwalled carbon nanotubes of diameters up to 10 nm are also size dependent.

Journal Article↗
PubMed2005

Comparative and functional genomic analyses of the pathogenicity of phytopathogen Xanthomonas campestris pv. campestris.

Xanthomonas campestris pathovar campestris (Xcc) is the causative agent of crucifer black rot disease, which causes severe losses in agricultural yield world-wide. This bacterium is a model organism for studying plant-bacteria interactions. We sequenced the complete genome of Xcc 8004 (5,148,708 bp), which is highly conserved relative to that of Xcc ATCC 33913. Comparative genomics analysis indicated that, in addition to a significant genomic-scale rearrangement cross the replication axis between two IS1478 elements, loss and acquisition of blocks of genes, rather than point mutations, constitute the main genetic variation between the two Xcc strains. Screening of a high-density transposon insertional mutant library (16,512 clones) of Xcc 8004 against a host plant (Brassica oleraceae) identified 75 nonredundant, single-copy insertions in protein-coding sequences (CDSs) and intergenic regions. In addition to known virulence factors, full virulence was found to require several additional metabolic pathways and regulatory systems, such as fatty acid degradation, type IV secretion system, cell signaling, and amino acids and nucleotide metabolism. Among the identified pathogenicity-related genes, three of unknown function were found in Xcc 8004-specific chromosomal segments, revealing a direct correlation between genomic dynamics and Xcc virulence. The present combination of comparative and functional genomic analyses provides valuable information about the genetic basis of Xcc pathogenicity, which may offer novel insight toward the development of efficient methods for prevention of this important plant disease.

Bacterial Proteins↗
PubMed2005

Developmental expression of the high mobility group B gene in the amphioxus, Branchiostoma belcheri tsingtauense.

High-Mobility Group (HMG) B proteins are abundant and highly conserved non-histone proteins, which play an important architectural role in the assembly of nucleoprotein complexes and in the Regulation of transcription. These proteins have also been shown to play key roles during embryonic development and cell differentiation. Here we report a full-length cDNA sequence of the HMG protein, AmphiHMGB from Amphioxus. Sequence analysis indicates that this putative AmphiHMGB protein contains four domains: HMG-box A, HMG-box B, basic region, acidic carboxyl-terminal tail and a linker. Phylogenetic analysis suggests that AmphiHMGB falls outside the vertebrate clade. HMGB gene duplication occurred near the base of the vertebrate gene Clade. The dynamic expression of AmphiHMGB during embryonic development reveals for the first time that it may involve differentiation of neural ectoderm, mesoderm and endoderm in this animal.

Amino Acid Sequence↗
PubMedDate not supplied

Light field morphing using 2D features.

We present a 2D feature-based technique for morphing 3D objects represented by light fields. Existing light field morphing methods require the user to specify corresponding 3D feature elements to guide morph computation. Since slight errors in 3D specification can lead to significant morphing artifacts, we propose a scheme based on 2D feature elements that is less sensitive to imprecise marking of features. First, 2D features are specified by the user in a number of key views in the source and target light fields. Then the two light fields are warped view by view as guided by the corresponding 2D features. Finally, the two warped light fields are blended together to yield the desired light field morph. Two key issues in light field morphing are feature specification and warping of light field rays. For feature specification, we introduce a user interface for delineating 2D features in key views of a light field, which are automatically interpolated to other views. For ray warping, we describe a 2D technique that accounts for visibility changes and present a comparison to the ideal morphing of light fields. Light field morphing based on 2D features makes it simple to incorporate previous image morphing techniques such as nonuniform blending, as well as to morph between an image and a light field.

Algorithms↗
PubMedDate not supplied

Decorating surfaces with bidirectional texture functions.

We present a system for decorating arbitrary surfaces with bidirectional texture functions (BTF). Our system generates BTFs in two steps. First, we automatically synthesize a BTF over the target surface from a given BTF sample. Then, we let the user interactively paint BTF patches onto the surface such that the painted patches seamlessly integrate with the background patterns. Our system is based on a patch-based texture synthesis approach known as quilting. We present a graphcut algorithm for BTF synthesis on surfaces and the algorithm works well for a wide variety of BTF samples, including those which present problems for existing algorithms. We also describe a graphcut texture painting algorithm for creating new surface imperfections (e.g., dirt, cracks, scratches) from existing imperfections found in input BTF samples. Using these algorithms, we can decorate surfaces with real-world textures that have spatially-variant reflectance, fine-scale geometry details, and surfaces imperfections. A particularly attractive feature of BTF painting is that it allows us to capture imperfections of real materials and paint them onto geometry models. We demonstrate the effectiveness of our system with examples.

Algorithms↗