Biomedical subjects

Lilly Linder

Publications and source records attributed to Lilly Linder.

6 recordsLinked to original sources

PubMed2006

Angiotensin II decreases the renal MRI blood oxygenation level-dependent signal.

Acute experimental reduction of renal blood flow decreases the renal blood oxygenation level-dependent (BOLD) MRI signal in animals. Angiotensin II also reduces renal blood flow, but the ability of BOLD MRI to dynamically detect this response has not yet been investigated in humans. Six healthy male volunteers underwent an individual dose-finding study to identify the intravenous doses of angiotensin II, norepinephrine, and sodium nitroprusside necessary to induce a 15-mm Hg peak mean arterial blood pressure change. MRI studies followed within 3 weeks, when angiotensin II (8.8+/-1.4 ng/kg), norepinephrine (52+/-12 ng/kg), and sodium nitroprusside (2.0+/-0.3 microg/kg) were given twice in an unblocked, randomized sequence while imaging experiments were performed on a 1.5-T Siemens Sonata. A multiecho echo-planar imaging sequence was used to acquire T2* maps with a temporal resolution of 1 respiratory cycle. Averaged over a renal cortex dominated region of interest, angiotensin II caused a shortening of T2* between 6% and 10%. Sodium nitroprusside and norepinephrine, although of equal potency concerning blood pressure responses, did not alter the renal BOLD signal. The renal BOLD response to angiotensin II appeared with short onset latency (as early as 10 seconds after peripheral intravenous angiotensin II bolus administration) suggesting that this response is a consequence of altered perfusion rather than increased renal oxygen consumption. The methods described here are suitable to assess renal responsiveness to angiotensin II and may, thus, be of great value in human hypertension research.

Adult↗

PubMed2005

Effect of water deprivation on cognitive-motor performance in healthy men and women.

Whether mental performance is affected by slowly progressive moderate dehydration induced by water deprivation has not been examined previously. Therefore, objective and subjective cognitive-motor function was examined in 16 volunteers (8 females, 8 males, mean age: 26 yr) twice, once after 24 h of water deprivation and once during normal water intake (randomized cross-over design; 7-day interval). Water deprivation resulted in a 2.6% decrease in body weight. Neither cognitive-motor function estimated by a paced auditory serial addition task, an adaptive 5-choice reaction time test, a manual tracking test, and a Stroop word-color conflict test nor neurophysiological function assessed by auditory event-related potentials P300 (oddball paradigm) differed (P > 0.1) between the water deprivation and the control study. However, subjective ratings of mental performance changed significantly toward increased tiredness (+1.0 points) and reduced alertness (-0.9 points on a 5-point scale; both: P < 0.05), and higher levels of perceived effort (+27 mm) and concentration (+28 mm on a 100-mm scale; both: P < 0.05) necessary for test accomplishment during dehydration. Several reaction time-based responses revealed significant interactions between gender and dehydration, with prolonged reaction time in women but shortened in men after water deprivation (Stroop word-color conflict test, reaction time in women: +26 ms, in men: -36 ms, P < 0.01; paced auditory serial addition task, reaction time in women +58 ms, in men -31 ms, P = 0.05). In conclusion, cognitive-motor function is preserved during water deprivation in young humans up to a moderate dehydration level of 2.6% of body weight. Sexual dimorphism for reaction time-based performance is present. Increased subjective task-related effort suggests that healthy volunteers exhibit cognitive compensating mechanisms for increased tiredness and reduced alertness during slowly progressive moderate dehydration.

Adult↗

PubMed2004

Acute hyperhomocysteinemia decreases NO bioavailability in healthy adults.

Hyperhomocysteinemia is associated with decreased vascular reactivity and increased cardiovascular morbidity. Oxidative stress and reduced NO bioavailability have been proposed as a mechanism for the adverse effects of chronically elevated plasma homocysteine levels. Recent studies suggest that acute elevations of plasma homocysteine may also impair endothelial function and vasodilation, however, the mechanism is not clear. In the present study, we investigated whether moderate hyperhomocysteinemia after methionine loading decreases NO bioavailability, increases oxidative stress, and impairs receptor-mediated NO-dependent venodilation in healthy adults. After oral methionine loading (0.1g/kg), mean homocysteine concentrations increased 3.2-fold, from 6.9 +/- 0.5 to 27.8 +/- 1.9 micromol/l (n = 16), whereas plasma NO(x) concentrations, an indicator of NO release, were decreased by 12% compared to placebo treatment (P = 0.005). Vitamin E levels in freshly isolated low density lipoprotein (LDL), a sensitive marker of LDL oxidation, and LDL lipid (hydro)peroxide levels were unchanged after methionine loading. Endothelium-dependent venodilation induced by bradykinin was reduced by 18% during hyperhomocysteinemia (P = 0.06). Taken together our data suggest that the reduced NO bioavailability was likely due to decreased NO synthesis and release rather than to NO destruction by oxidative stress.

Adult↗

PubMed2004

Mental relaxation improves long-term incidental visual memory.

Experimental evidence has linked increased arousal to enhanced memory retention. There is also evidence that procedures reducing arousal, i.e., mental relaxation, might improve memory, but conflicting results have been reported. To clarify this issue, we studied the effects of a single session of relaxation training on incidental visual long-term memory. Thirty-two relaxation-naive subjects viewed 280 slides without being told that there would be subsequent memory testing. Afterwards, subjects listened to a 12 min relaxation tape; 16 subjects relaxed by following the instructions (relaxation group), and the other 16 subjects pressed a button whenever a body part was mentioned (control group). While listening to the relaxation tape, high frequency heart rate variability (HRV) was greater and low frequency HRV was lower in the relaxation group, implying effective relaxation and increasing parasympathetic activation. The relaxation group had superior memory retention 4 weeks later (p = .004), indicating enhancement of long-term memory performance. This effect could not be explained by retroactive interference experienced in the control group because short-term memory performance immediately after the tape was slightly better in the control group. Retention of materials acquired after the relaxation session remained unaffected, suggesting relaxation has retrograde effects on memory consolidation. Our data demonstrate a favorable influence of relaxation on at least this aspect of learning. Our data also extend previous knowledge on the beneficial effects of ascending parasympathetic stimulation on memory retention in that enhanced long-term memory consolidation may also occur in the presence of central and descending parasympathetic activation triggered by willful psychomotor activity.

Adult↗

PubMed2004

Increased high-frequency heart rate variability during insulin-induced hypoglycaemia in healthy humans.

Despite causing sympathetic activation, prolonged hypoglycaemia produces little change in HR (heart rate) in healthy young adults. One explanation could be concurrent parasympathetic activation, resulting in unchanged net effects of autonomic influences. In the present study, hypoglycaemic (2.7 mmol/l) and normoglycaemic (4.7 mmol/l) hyperinsulinaemic clamp studies were performed after normoglycaemic baseline clamp periods with 15 healthy volunteers (seven male; mean age, 27 years) on two occasions in a randomized single-blind cross-over design. Non-invasive indices of cardiac autonomic activity and hormones were measured at baseline and 1 h after the beginning of hypoglycaemia or control normoglycaemia. Plasma insulin levels and mean HR were similar during both conditions. During hypoglycaemia, there was a 485% increase in plasma adrenaline (epinephrine). A shortening of the pre-ejection period by 45% suggested strong sympathetic cardiac activation. High-frequency (0.15-0.45 Hz) HRV (HR variability) increased, indicating a concomitant increase in parasympathetic tone. Thus, during hypoglycaemia-induced sympathetic cardiac activation in healthy adults, parasympathetic mechanisms are involved in stabilizing mean HR.

Adult↗

PubMed2003

Cognitive and psychomotor function in hypoglycemia: response error patterns and retest reliability.

Hypoglycemia has been shown to impair cognitive and psychomotor function, but it has been unclear which measures are most reliable and sensitive for detecting these effects. In a single-blind repeated measures design, healthy young adults (N=17, 8 male, mean age 27 years) performed three PC-based cognitive and psychomotor function tests: a paced auditory serial addition task (PASAT), an adaptive five-choice reaction time test (CRTT), and a manual tracking test on two occasions 4 weeks apart. In each session, a hyperinsulinemic clamp method was used during a normoglycemic (plasma glucose: 4.7 mmol/l) baseline testing period, followed in one session by a normoglycemic target testing period, and in the other session by a hypoglycemic (2.7 mmol/l) target testing period. All cognitive and psychomotor function measures showed high test-retest reliability (r ranging from.69 to.95) and sensitivity to hypoglycemia (P<.01). A new finding is that on the PASAT, hypoglycemia appears to differentially increase the rate of omission errors more than it increases false responses. Data on PASAT reaction time (RT) are also presented.

Adult↗