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Lisa A Holland

Publications and source records attributed to Lisa A Holland.

8 recordsLinked to original sources
PubMed2005

Probing affinity via capillary electrophoresis: advances in 2003-2004.

This review addresses recent advances in capillary electrophoresis of biological-based molecular interaction from a broader perspective, based on applications reported during the period 2003-2004. These capillary electrophoresis-based studies of molecular interactions include affinity capillary electrophoresis, electrokinetic chromatography, and free zone electrophoresis. The review is written as a general synopsis of applications and does not cover the theory or protocol involved in the implementation of the analyses.

Carbohydrates↗
PubMed2005

Rapid assessment of beta-asarone content of Acorus calamus by micellar electrokinetic capillary chromatography.

This report outlines a rapid, reproducible method for the determination of beta-asarone, a known carcinogen, using micellar electrokinetic capillary chromatography (MEKC)-UV-vis absorbance and a simple alcohol extraction. The MEKC method is based on a running buffer comprised of 100 mM sodium dodecyl sulfate (SDS), pH 10. The method is reproducible and provides baseline separation of alpha-asarone and beta-asarone. This protocol was used to determine the beta-asarone content of Acorus calamus rhizome of a diploid variety harvested from the wetlands of the United States and the triploid variety from India obtained commercially. The results indicate raw product that originated from India contained 4.4% w/w beta-asarone, while that from the United States contained 0.2% w/w beta-asarone. Neither sample contained detectable concentrations of alpha-asarone. This is the first report of the use of MEKC to determine asarone in a natural source.

Acorus↗
PubMed2005

Recent advances in micellar electrokinetic chromatography.

This review contains nearly 200 reference citations, and covers advances in electrokinetic capillary chromatography based on micelles, including stabilized micelle complexes, polymeric and mixed micelles from 2003-2004. Detection strategies, analyte determinations, and applications in micellar electrokinetic capillary chromatography (MEKC) are discussed. Information regarding methods of analyte concentration, analyte specific analyses, and nonstandard micelles has been summarized in tabular form to provide a means of rapid access to information pertinent to the reader.

Chromatography, Micellar Electrokinetic Capillary↗
PubMed2004

Determination of dissociation constants for a heparin-binding domain of amyloid precursor protein and heparins or heparan sulfate by affinity capillary electrophoresis.

Dynamic affinity capillary electrophoresis (ACE) was used for determining the binding constants between heparin-like glycosaminoglycans and the (96-110) heparin-binding domain of amyloid precursor protein (APP). The migration time shift of the (96-110) APP peptide was monitored as the concentration of heparin was increased in the background electrolyte. The compounds investigated included low-molecular-weight heparin, porcine mucosa heparin, and heparan sulfate. Change in mobility as a function of glycosaminoglycan concentration was plotted using both linear regression (Scatchard analysis) and nonlinear regression. Dissociation constants (K(d)) were determined and compared for both sets of analyses with the low-molecular-weight heparin giving the most reproducible results and best fit with a K(d) value of 3.9 microM.

Amyloid beta-Protein Precursor↗
PubMed2004

Membrane-mediated capillary electrophoresis: interaction of cationic peptides with bicelles.

Electrokinetic capillary chromatography is applied to determine the membrane affinity of peptides using both 1,2-dihexanoyl-sn-glycero-3-phosphocholine (DHPC) micelles and DHPC/1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) bicelles under controlled conditions. The effect of temperature and the bicelle q value in surface association with cationic peptides is studied. The cationic peptides selected have a well-defined membrane structure (indolicidin), induced secondary structure (melittin, magainin 2), or do not possess classical secondary structure (atrial natriuretic peptide (ANP) 1-28, 4-28, 5-27). Electrokinetic capillary chromatography facilitated by DMPC and DHPC additives provides a rapid means of estimating lipophilicity and screening for peptides that have membrane affinity.

Antimicrobial Cationic Peptides↗
PubMed2003

Bilayered phospholipid micelles and capillary electrophoresis: a new additive for electrokinetic chromatography.

Phospholipid micelles and bilayered micelles (bicelles) were investigated as a new media for electrokinetic chromatography. The benefit to using these additives for micellar electrokinetic chromatography (MEKC) is the potential of a simple bilayer membrane model operated with fast analysis time, and low sample injection volumes. The system is used to separate peptides/protein and is tested with a series of beta-blockers. The results suggest that bicelle electrokinetic chromatography provides selectivity and holds potential as an alternative approach to modeling membrane phenomenon.

Adrenergic beta-Antagonists↗
PubMed2002

Amperometric and voltammetric detection for capillary electrophoresis.

The focus of this article is amperometric and voltammetric detection coupled with capillary electrophoresis. Fundamental concepts and progress in the field of capillary electrophoresis with electrochemical detection (CEEC) that have occurred within the past three years, including new methodologies and unique applications, are highlighted. This review contains 95 references.

Animals↗