Study on vitamin K3-cyclodextrin inclusion complex and analytical application.
The inclusion interaction of the complexes between Vitamin K(3) (VK(3)) and beta-cyclodextrin (beta-CD), hydroxypropyl-beta-cyclodextrin (HP-beta-CD) and sulfobutylether-beta-cyclodextrin (SBE-beta-CD) were studied by using steady-state fluorescence measurements. The various factors affecting the inclusion process were examined in detail. The formation constants and inclusion stoichiometry for VK(3)-CDs were determined. The results showed that the inclusion ability of beta-CD and its derivatives was the order: SBE-beta-CD>HP-beta-CD>beta-CD. The related inclusion mechanism is proposed to explain the inclusion process. A method of determining VK(3) was established with the linear range was 2.5 x 10(-6)-5.0 x 10(-4) M, and was used to determine the VK(3) tablets. The recoveries were in the range of 97.52-103.5%. The results were satisfactory.