Biomedical subjects

Liv Drolsum

Publications and source records attributed to Liv Drolsum.

8 recordsLinked to original sources

PubMed2006

A novel method for preserving cultured limbal epithelial cells.

AIM: To investigate organ culture preservation of cultured limbal epithelial cells in order to enhance the availability of tissue-engineered epithelia that are used to treat patients with limbal stem cell deficiency. METHODS: Limbal epithelial cells were cultured for 3 weeks on intact amniotic membrane fastened to a polyester membrane carrier. The cultured epithelia were stored for 1 week at 23 degrees C in organ culture medium. The preserved epithelia were then examined using a colorimetric cell viability assay, light microscopy and immunohistochemistry. RESULTS: The viability of the preserved epithelia was 84% (20%), and no statistically significant difference was found compared with non-preserved epithelia. In general, the cell borders were maintained, the nuclei showed no sign of degeneration, and the original layered structure was preserved. Mild intercellular oedema was occasionally observed. Expression of p63, K19 and vimentin was maintained. CONCLUSIONS: Cultured limbal epithelial cells can be preserved in organ culture medium for 1 week at room temperature, while maintaining the original layered structure and undifferentiated phenotype.

Amnion↗

PubMed2006

Longterm follow-up after deep sclerectomy in patients with pseudoexfoliative glaucoma.

PURPOSE: To compare the longterm results in pseudoexfoliative glaucoma and primary open-angle glaucoma (POAG) following deep sclerectomy with implant. METHODS: A total of 28 pseudoexfoliative glaucoma eyes and 27 POAG eyes were consecutively included in the study. Intraocular pressure (IOP) and number of glaucoma medications were compared before surgery and at every postoperative visit. Most patients included had very advanced glaucoma and were using multiple therapies (mean 3.3 and 3.6 in the pseudoexfoliative glaucoma and POAG groups, respectively) and the duration of the disease was more than 8 years in both groups. The definition of complete success was the achievement of IOP < 19 mmHg without therapy. RESULTS: After a mean follow-up time of 45 months in the pseudoexfoliative glaucoma group and 43 months in the POAG group, 50.0% of the pseudoexfoliative glaucoma group were considered to represent complete success compared with 33.3% of the POAG group (NS). In survival analyses controlling for covariates, it was found that patients with pseudoexfoliative glaucoma had a higher rate of success over time than POAG patients (p = 0.014). Furthermore, the duration of glaucoma prior to surgery had a negative influence on the success rate (p = 0.034). Four years after surgery, the mean IOP was 15.5 +/- 5.3 mmHg in the pseudoexfoliative glaucoma group and 13.6 +/- 3.5 mmHg in the POAG group (NS). The mean number of medications used by each group at that time was 1.7 +/- 1.5 and 1.2 +/- 1.1, respectively (NS). CONCLUSION: The present study suggests that deep sclerectomy is a safe procedure in pseudoexfoliative glaucoma. Half of the patients were regulated without therapy after 45 months.

Aged↗

PubMed2006

Iris microhaemangiomas and idiopathic juxtafoveolar retinal telangiectasis.

We describe a case of iris microhaemangiomas (iris vascular tufts) causing spontaneous hyphaema and where examination showed that the patient also had idiopathic juxtafoveolar retinal telangiectasis. A systematic search through PubMed, EMBASE and reference lists revealed a total of 90 reported cases of iris microhaemangiomas. To our knowledge, no previous reports have described concurrent findings of iris microhaemangiomas and idiopathic juxtafoveolar retinal telangiectasis in a patient. Both conditions are acquired vascular diseases, but their pathogenesis and aetiologies are unknown. Iris microhaemangiomas are important clinically because they are occasionally a cause of spontaneous hyphaema and may induce transient elevation of intraocular pressure.

Aged↗

PubMed2006

Use of amniotic membrane as an adjuvant in refractory glaucoma.

PURPOSE: To evaluate the results after amnion-shielded trabeculectomy with concomitant use of mitomycin C. METHODS: The study comprised patients with severely refractory glaucomas who had previously undergone two or more regular trabeculectomies with mitomycin C and, in one case, cyclodestructive cryotherapy. The patients were admitted for surgery between May 2003 and May 2004. Trabeculectomy was performed through a limbus-based incision in nine eyes of nine patients. Cryopreserved human amniotic membranes obtained from our biobank were impregnated with 0.4 mg/ml mitomycin C and washed for 2 mins in balanced salt solution. One membrane was sutured to the scleral surface and partly positioned under the scleral flap, and another was secured to subconjunctival tissue. RESULTS: The mean preoperative intraocular pressure (IOP) was 32.2 mmHg (range 22-44 mmHg). After a follow-up of 6-18 months (mean 9.8 months), the mean IOP was 16.4 mmHg (range 11-26 mmHg). The mean number of glaucoma medications was reduced from 2.4 preoperatively to 1.4 postoperatively. There were no devastating complications. CONCLUSIONS: Amnion-shielded trabeculectomy is a procedure that should be evaluated as an option in refractory glaucoma.

Amnion↗

PubMed2003

Long-term follow-up of secondary flexible, open-loop, anterior chamber intraocular lenses.

PURPOSE: To evaluate the long-term results of secondary implantation of modern flexible, open-loop, anterior chamber intraocular lenses (IOLs). SETTING: Department of Ophthalmology, National University Hospital, Oslo, Norway. METHODS: This prospective study comprised 18 patients (20 aphakic eyes) who had secondary IOL implantation from January 1990 to May 1992. In an earlier report, these patients were followed for 4 months. The patients were reexamined in October 1998. The mean follow-up was 93.8 months +/- 6.0 (SD). Two patients had died. No others were lost to follow-up. The intraocular pressure (IOP) in the remaining 16 patients (18 eyes) was compared to that 4 months postoperatively. Visual acuity was compared to the preoperative measurements. A complete slitlamp examination was performed, and complications were noted. RESULTS: There was no statistical decrease in visual acuity during the follow-up. The mean IOP was 16.6 +/- 5.7 mm Hg (range 8 to 27 mm Hg) in 1998 and 13.4 +/- 3.4 mm Hg (range 7 to 19 mm Hg) 4 months postoperatively. The difference was statistically significant (P =.037). Corneal pathology was seen in 3 eyes, 1 of which had developed corneal decompensation. One patient had surgery for retinal detachment 4 years postoperatively. The pupil was deformed in 5 eyes. CONCLUSIONS: This long-term study demonstrates that secondary implantation of flexible, open-loop, anterior chamber IOLs is safe in selected eyes. Anterior chamber IOLs should not be used in eyes with corneal problems and should be used with caution in eyes with glaucoma. These patients require close, continued observation to look for late complications such as IOP elevation.

Adult↗

PubMed2003

Conversion from trabeculectomy to deep sclerectomy. Prospective study of the first 44 cases.

PURPOSE: To evaluate the outcomes of conversion from trabeculectomy to deep sclerectomy with placement of a drainage implant. SETTING: Department of Ophthalmology, Hospital of Buskerud, Drammen, Norway. METHODS: Deep sclerectomy was performed in 41 patients (44 eyes) from November 1999 to January 2002. An absorbable implant (AquaFlow((R)), Staar Surgical) and a nonabsorbable implant (T.Flux((R)), IOLTech Laboratories) were used in 75% and 25% of eyes, respectively. Patients with open-angle glaucoma who needed filtering surgery were enrolled consecutively in a prospective study. Patients with 2 or more previous filtering surgeries or neovascular glaucoma were excluded, as were children. Of the original 48 eyes, accidental perforation of the trabeculo-Descemet's membrane occurred in 8.3%; these eyes were excluded from the study. The severity of optic nerve damage was considered in the decision to start therapy. An intraocular pressure (IOP) above 22 mm Hg was always treated. RESULTS: After a mean follow-up of 12.9 months +/- 7.1 (SD), 61.4% of eyes were controlled without therapy. When eyes with previous surgery were excluded, the rate increased to 66.7% after a mean follow-up of 13.8 +/- 7.2 months. The mean IOP preoperatively and after 12 months was 27.9 +/- 6.9 mm Hg and 14.6 +/- 3.5 mm Hg, respectively (P<.001). The mean number of medications decreased from 3.4 +/- 1.0 preoperatively to 0.6 +/- 1.1 12 months postoperatively (P<.001). A reoperation was required in 2 cases (4.5%). There were no complications related to hypotony or other significant complications. CONCLUSIONS: This study shows that conversion from trabeculectomy to deep sclerectomy with placement of an implant is safe. The postoperative IOP reduction was acceptable, and no significant complications occurred.

Adult↗

PubMed2003

A comparison of Nd:YAG capsulotomy rate in two different intraocular lenses: AcrySof and Stabibag.

PURPOSE: To evaluate the incidence of posterior capsule opacification after implantation of two different intraocular lenses (IOLs), AcrySof and Stabibag, by comparing the neodymium:YAG (Nd:YAG) laser capsulotomy rates. METHODS: The medical records of 596 patients (705 eyes) who underwent phacoemulsification and posterior chamber IOL implantation using either AcrySof (n = 335) or Stabibag (n = 370) IOLs during a 1-year (1999) period were reviewed. Eyes with secondary cataract, combined procedures or operative complications, such as capsule tears, were excluded from the study. The subjects' age, sex, surgeon (two surgeons), and time for Nd:YAG laser capsulotomy were recorded. RESULTS: The mean follow-up was 23 months in both IOL groups. There were no statistically significant differences within the two IOL groups regarding sex distribution or surgeon. The mean age in the AcrySof group was 77.0 years compared to 79.2 years in the Stabibag group (p = 0.001). Nd:YAG laser capsulotomy was performed in 7.6% of patients in the Stabibag group compared to 2.7% in the AcrySof group (i.e. at a significantly higher rate) (p = 0.004). Survival analyses demonstrated that age and type of IOL were the only independent predictors of the incidence of capsulotomy. CONCLUSIONS: This study shows that the frequency of eyes with posterior capsule opacification needing capsulotomy was significantly higher in eyes with Stabibag IOLs compared to eyes with AcrySof IOLs.

Acrylic Resins↗

PubMed2003

Deep sclerectomy in patients with capsular glaucoma.

PURPOSE: To compare the results of deep sclerectomy in capsular glaucoma (CG) with those in primary open-angle glaucoma (POAG). METHODS: This consecutive, prospective study comprised 24 CG patients (28 eyes) and 25 POAG patients (29 eyes) who underwent deep sclerectomy. Two different implants were used: either an absorbable collagen implant (Aqua-Flow) or a non-absorbable hydrophilic acrylic implant (T-Flux). The number of glaucoma medications, intraocular pressure (IOP) and complications were compared postoperatively. The definition of complete success was IOP below 19 mmHg without therapy. RESULTS: After a mean follow-up of 19.9 +/- 10.9 months (range 6-36 months) in the CG group and 16.2 +/- 10.0 months (range 6-36 months) in the POAG group, complete success was seen in 60.7% and in 37.9% of eyes, respectively (p=0.085). After adjustments for disparities in baseline characteristics, survival analysis demonstrated that success rates were better over time in CG eyes than in POAG eyes (p=0.038). At all time-points, except at 24 months, the IOP was lower in the CG group than in the POAG group. This difference was statistically significant at 1 week (p=0.050) and 3 months (p=0.006). At 18 months, the mean decrease in number of medications was 77.3% in the CG group and 65.9% in the POAG group (not statistically significant). Levelled hyphema occurred more frequently in CG eyes (35.7%) than in POAG eyes (13.8%). CONCLUSION: Capsular glaucoma patients had significantly higher success rates over time than POAG patients following deep sclerectomy with implant.

Acrylic Resins↗