Loranelle L Lockyear | PubMed Health

PubMed2006

Reversible, room temperature bonding of glass devices for microfluidics.

This chapter describes a procedure for bonding glass microdevice substrates to their top plates by contact alone. This method results in devices that are robustly bonded but that can be separated, cleaned, and reused. For glass chips that have been used for applications involving the transport of hard particles, cells, or other biological material, reversible bonding provides a way of increasing the chip's lifetime and utility. Glass microdevices that are cleaned following this procedure can be successfully used many times for electrophoresis or pressure-driven applications.

Glass↗

PubMed2006

Solid-phase extraction in packed beds on glass microdevices.

This chapter provides a detailed description of a network of channels that includes a chamber for trapping beads in a microfluidic device. Instructions are included for the packing and use of the bead bed for solid-phase extraction (SPE). The SPE procedure may be used, e.g., as a filter to clean up a dirty sample prior to analysis, or as a means of pre-concentration for a dilute sample. Once the bead bed is in place, it may be used multiple times without sample breakthrough.

Glass↗

PubMed2004

Synthesis, characterization, and photochemical and computational investigations of Ru(II) heterocyclic complexes containing 2,6-dimethylphenylisocyanide (CNx) ligand.

The isocyanide ligand forms complexes with ruthenium(II) bis-bipyridine of the type [Ru(bpy)(2)(CNx)Cl](CF(3)SO(3)) (1), [Ru(bpy)(2)(CNx)(py)](PF(6))(2) (2), and [Ru(bpy)(2)(CNx)(2)](PF(6))(2) (3) (bpy = 2,2'-bipyridine, py = pyridine, and CNx = 2,6-dimethylphenylisocyanide). The redox potentials shift positively as the number of CNx ligands increases. The metal-to-ligand charge-transfer (MLCT) bands of the complexes are located at higher energy than 450 nm and blue shift in proportion to the number of CNx ligands. The complexes are not emissive at room temperature but exhibit intense structured emission bands at 77 K with emission lifetimes as high as 25 micros. Geometry optimization of the complexes in the singlet ground and lowest-lying triplet states performed using density functional theory (DFT) provides information about the orbital heritage and correlates with X-ray and electrochemical results. The lowest-lying triplet-state energies correlate well with the 77 K emission energies for the three complexes. Singlet excited states calculated in ethanol using time-dependent density functional theory (TDDFT) and the conductor-like polarizable continuum model (CPCM) provide information that correlates favorably with the experimental absorption spectra in ethanol.

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