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Biomedical subjects

Louis Tong

Publications and source records attributed to Louis Tong.

At least 19 recordsLinked to original sources
PubMed2006

Atropine for the treatment of childhood myopia.

PURPOSE: To evaluate the efficacy and safety of topical atropine, a nonselective muscarinic antagonist, in slowing the progression of myopia and ocular axial elongation in Asian children. DESIGN: Parallel-group, placebo-controlled, randomized, double-masked study. PARTICIPANTS: Four hundred children aged 6 to 12 years with refractive error of spherical equivalent -1.00 to -6.00 diopters (D) and astigmatism of -1.50 D or less. INTERVENTION: Participants were assigned with equal probability to receive either 1% atropine or vehicle eye drops once nightly for 2 years. Only 1 eye of each subject was chosen through randomization for treatment. MAIN OUTCOME MEASURES: The main efficacy outcome measures were change in spherical equivalent refraction as measured by cycloplegic autorefraction and change in ocular axial length as measured by ultrasonography. The primary safety outcome measure was the occurrence of adverse events. RESULTS: Three hundred forty-six (86.5%) children completed the 2-year study. After 2 years, the mean progression of myopia and of axial elongation in the placebo-treated control eyes was -1.20+/-0.69 D and 0.38+/-0.38 mm, respectively. In the atropine-treated eyes, myopia progression was only -0.28+/-0.92 D, whereas the axial length remained essentially unchanged compared with baseline (-0.02+/-0.35 mm). The differences in myopia progression and axial elongation between the 2 groups were -0.92 D (95% confidence interval, -1.10 to -0.77 D; P<0.001) and 0.40 mm (95% confidence interval, 0.35-0.45 mm; P<0.001), respectively. No serious adverse events related to atropine were reported. CONCLUSIONS: Topical atropine was well tolerated and effective in slowing the progression of low and moderate myopia and ocular axial elongation in Asian children.

Administration, Topical↗
PubMed2006

Targeted inhibition of p57 and p15 blocks transforming growth factor beta-inhibited proliferation of primary cultured human limbal epithelial cells.

PURPOSE: To evaluate the role of cyclin-dependent kinase inhibitors p57 and p15 in transforming growth factor (TGF)-beta1 or TGF-beta2 inhibited proliferation of primary cultured human limbal epithelial cells using short interfering RNA (siRNA). METHODS: Primary cultured human limbal epithelial cells were treated with TGF-beta1 or TGF-beta2 for 6 and 24 h, and total RNA extracted for RT-PCR and real-time PCR using primers for p21, p27, and p57 (CipP/Kip family) and p15 and p19 (INK4 family). Proteins were extracted for western blot analysis of p57 and p15. For RNA interference, primary cultured human limbal epithelial cells were transfected with annealed double-stranded siRNA (67 nM) specific for p57, p15, or siRNA-Fluorescein (siRNA-F; as a negative control) followed by treatment with TGF-beta1 or TGF-beta2 at 1 ng/ml. P57 and p15 were quantitatively detected by real-time PCR and western blot; and immunolocalized by immunofluorescent staining. The effects of TGF-beta1 or TGF-beta2 on cell proliferation were evaluated by BrdU incorporation and MTT assay. RESULTS: TGF-beta1 or TGF-beta2 significantly inhibited primary cultured human limbal epithelial cell proliferation measured by BrdU incorporation and MTT assay. TGF-beta1 or TGF-beta2 upregulated the expression of p57 and p15 mRNA and protein, but did not effect the expression of p19, p21, or p27. The siRNA transfection efficiency of these cells was 75% and no cellular toxicity was observed by 24 h. The TGF-beta1 or TGF-beta2 stimulated expression of p57 and p15 mRNA were markedly blocked by siRNA-p57 or siRNA-p15, respectively, but not by siRNA-F. The TGF-beta1 or TGF-beta2 suppression of epithelial proliferation measured by BrdU incorporation and MTT generation was increased to near normal levels by siRNA-p57 or siRNA-p15. Western blot and immunofluorescent staining showed that levels of p57 and p15 proteins were equally reduced in the cytoplasm and nucleus. CONCLUSIONS: These findings demonstrate that TGF-beta1 and/or TGF-beta2 inhibit proliferation of primary cultured human limbal epithelial cells and that p57 and p15 play roles in this process.

Cell Proliferation↗
PubMed2006

Longitudinal study of anisometropia in Singaporean school children.

PURPOSE: The purpose of this study was to document the incidence rates of anisometropia, year-by-year prevalences, changes in the intereye difference in spherical equivalent (SE), and its association with myopia progression and axial length changes in a cohort of Singaporean school children. METHODS: This is a prospective cohort study of Singaporean school children (n = 1979) aged 7 to 9 years who were examined annually with cycloplegic refraction and ultrasonography over a 3-year period. RESULTS: In the 1908 children without anisometropia at commencement, the 3-year cumulative incidence rate of anisometropia (difference in SE at least 1.0 D) was 144 (7.55%; 95% CI: 6.42-8.85). The mean intereye difference in SE in all children at baseline was 0.29 +/- 0.46 D (SD: 0.46) and increased to 0.44 D (0.59) on the last examination. On the initial examination, 3.6% (95% CI: 2.8-4.4) or 71 children had anisometropia. Of the 59 of 71 children who completed all examinations, only 3 (5.1%) had an increase in the intereye difference in SE by at least 0.5 D, whereas 2 (3.4%) had a decrease of at least 0.5D. The mean intereye difference in SE was stable between visits. The change in intereye difference in SE correlated with the change in intereye axial length (r = 0.43). Compared with the isometropic children, each eye of the anisometropic children had a higher rate of progression of myopia. CONCLUSIONS: The 3-year incidence of anisometropia was 7.55% in these young Singaporean children. Although the frequency of anisometropia increased with time, the difference in SE between eyes tended to remain stable.

Anisometropia↗
PubMed2006

Expression and regulation of cornified envelope proteins in human corneal epithelium.

PURPOSE: Stratified squamous epithelial cells assemble a specialized protective barrier structure on their periphery, termed the cornified envelope. The purpose of this study was to evaluate the presence and distribution of cornified envelope precursors in human corneal epithelium, their expression in human corneal epithelial cell cultures, and the effect of ultraviolet radiation (UVB) and transglutaminase (TG) inhibition on their expression. METHODS: Tissue distribution of small proline-rich proteins (SPRRs) and filaggrin and involucrin was studied in human cornea sections by immunofluorescence staining. Primary human corneal epithelial cells (HCECs) from limbal explants were used in cell culture experiments. A single dose of UVB at 20 mJ/cm2 was used to stimulate these cells, in the presence or absence of mono-dansyl cadaverine (MDC), a TG inhibitor. SPRR2 and involucrin protein levels were studied by immunofluorescence staining and Western blot analysis. Gene expression of 12 proteins was investigated by semiquantitative reverse transcription-polymerase chain reaction. RESULTS: In human cornea tissue, SPRR1, SPRR2, filaggrin, and involucrin protein expression were detected in the central and peripheral corneal and limbal epithelium. In HCECs, SPRR2 and involucrin proteins were detected in the cytosolic fraction, and involucrin levels increased after UVB. Both SPRR2 and involucrin levels accumulated in the presence of MDC. Nine genes including involucrin, SPRR (types 1A, 1B, 2A, 2B, and 3), late envelope protein (LEP) 1 and 16, and filaggrin were expressed by HCECs. SPRR 4, loricrin, and LEP 6 transcripts were not detected. UVB downregulated SPRR (2A, 2B) and LEP 1 transcripts. CONCLUSIONS: Various envelope precursors are expressed in human corneal epithelium and in HCECs, acute UVB stress differentially alters their expression in HCECs. The expression of envelope precursors and their rapid modulation by UVB supports the role of these proteins in the regulation of ocular surface stress. TG function may be relevant in the regulation of soluble precursors in UVB-stimulated corneal epithelium.

Adolescent↗
PubMed2006

Transglutaminase participates in UVB-induced cell death pathways in human corneal epithelial cells.

PURPOSE: Ultraviolet light (UVB) is known to cause apoptosis in human corneal epithelial cells. This study evaluates the role of transglutaminase in regulating tumor necrosis factor (TNF) receptor clustering as well as caspase activation in UVB-induced apoptosis in human corneal epithelial cells. METHODS: A human corneal epithelial cell line was used. A single dose of UVB (20 mJ/cm2) was used as a stimulus. Cell viability and cell death were investigated by MTT, terminal deoxynucleotidyl transferase-mediated dUTP-digoxigenin nick end labeling (TUNEL), and caspase-3 assays. Immunofluorescent staining was used to investigate TNF receptor-I clustering at various time intervals after UVB. Short interfering RNA was used to knock down transglutaminase-2 expression. Fluorescein-cadaverine uptake was used to assess transglutaminase activity. A noncovalent peptide delivery system was used to transfect guinea pig liver transglutaminase into corneal epithelial cells. RESULTS: UVB increased transglutaminase activity, reduced cell viability, and increased TUNEL staining. UVB or TNF-alpha promoted TNF-receptor-I clustering, a process inhibited by the transglutaminase inhibitor, mono-dansyl cadaverine. UVB also increased activated caspase-3, in a manner suppressible by mono-dansyl cadaverine. Intracellular delivery of exogenous transglutaminase markedly increase caspase-3 activation compared with the vehicle control. CONCLUSIONS: Transglutaminase enzymatic activity is involved in corneal epithelial cell death after UVB and appears to participate in two steps regulating this process, clustering of TNF receptor-I and caspase-3 activation.

Apoptosis↗
PubMed2005

Prevalence of cataract in rural Indonesia.

PURPOSE: To describe the prevalence of cataract in adults in rural Sumatra, Indonesia. DESIGN: Population-based cross-sectional study. PARTICIPANTS: A random sample of all adults aged 21 years or older living in 3 rural villages in central Sumatra was assessed. Nine hundred nineteen of 1089 (84.4%) eligible adults participated. METHODS: A team of 7 ophthalmologists examined the anterior segment of both eyes using a portable slit lamp after pupil dilatation. Lens opacity was graded according to the Lens Opacities Classification System III (LOCS III). A structured questionnaire was used to collect data on education level and income. MAIN OUTCOME MEASURES: Cataract was defined as either a LOCS III nuclear region score of > or =4.0, cortical > or =4.0, or posterior subcapsular (PSC) cataract > or =2.0, in either eye. RESULTS: Two hundred one (21.9%) of 919 subjects were found to have cataract. The age-adjusted prevalence rate of cataract (including cataract surgery) was 23.0% (95% confidence interval, 20.8-25.2). The most common type of cataract for both genders (adjusted for age) was mixed (13%) followed by nuclear only (5.7%), and cortical only (4%). The prevalence rate of any cataract for adults aged 21 to 29 was 1.1%, increasing to 82.8% for those aged older than 60 years. Similar trends with age were noted for nuclear, cortical, and PSC cataract. Women had higher prevalence rates than men for all types of cataract except cortical. There was a trend of increasing prevalence of all types of cataract with decreasing education (P<0.001). CONCLUSIONS: Cataract prevalence in adults aged 21 years and older in rural Indonesia is among the highest reported in Southeast Asia. Despite this, there are inadequate resources available to manage this treatable disease. Allocation of resources to tackle the present burden of cataract would likely have large personal, social, and economic benefits.

Adult↗
PubMed2005

Stress-activated protein kinase signaling pathways in dry eye and ocular surface disease.

Inflammation is recognized as a key component in the pathogenesis of dry eye and a variety of ocular surface diseases. Stress-activated protein kinases have been identified as pathways signaling ocular surface stresses, such as increased tear film osmolarity and ultraviolet light exposure. Activation of these stress pathways results in transcription of stress related genes, including inflammatory cytokines (e.g. interleukin-1 and tumor necrosis factor (TNF)-alpha and matrix metalloproteinases (MMPs), such as MMP-9. Treatment of osmo-stressed cultured corneal epithelia with inhibitors of the stress associated kinase c-jun N-terminal kinases was found to decrease production of MMPs by these cells. These findings suggest that SAPKs may be key therapeutic targets for dry eye and ocular surface diseases.

Journal Article↗
PubMed2005

Incidence and progression of myopia in Singaporean school children.

PURPOSE: To determine the incidence and progression rates of myopia in young Singaporean children. METHODS: A prospective cohort study, the Singapore Cohort Study of the Risk Factors for Myopia (SCORM), was conducted in two schools in Singapore (1999-2002). Children aged 7 to 9 years (n=981) were followed up over a 3-year period. Cycloplegic autorefraction and biometry parameter measures were performed annually, according to the same protocol. RESULTS: The 3-year cumulative incidence rates were 47.7% (95% confidence interval [CI]: 42.2-53.3), 38.4% (95% CI: 31.4-45.4), and 32.4% (95% CI: 21.8-43.1) for 7-, 8-, and 9-year-old children, respectively. The 3-year cumulative incidence rates were higher in Chinese (49.5% vs. 27.2%) and in 7-year-old compared with 9-year-old children at baseline (47.7% vs. 32.4%), though the latter relationship was of borderline significance after adjustment for race, gender, amount of reading (books/week), and parental myopia (P=0.057). Premyopic children with greater axial lengths, vitreous chamber depths, and thinner lenses were more prone to the development of myopia, after controlling for age, gender, race, reading, and parental myopia. The 3-year mean cumulative myopia progression rates were -2.40 D (95% CI: -2.57 to -2.22) in 7-year-old myopic children, -1.97 (95% CI: -2.16 to -1.78) in 8-year-olds, and -1.71 (95% CI: -1.98 to -1.44) in 9-year-olds. CONCLUSIONS: Both the incidence and progression rates of myopia are high in Singaporean children.

Age Distribution↗
PubMed2004

Anisometropia in Singapore school children.

PURPOSE: To report the prevalence rates of anisometropia in a school population and determine the relative contribution of refractive power and axial length to the measured anisometropia. DESIGN: Population-based cross-sectional study. METHODS: Autorefraction, keratometry, and ultrasonography studies were made. SETTING: Three schools, located on the eastern, northern, and western part of Singapore. STUDY POPULATION: In all, 1,979 children aged 7 to 9 years were recruited for this study. The study sample included Chinese (n = 1,481), Malay (n = 324), and Asian Indian (n = 174) children; 720 subjects have myopia (spherical equivalent <= -0.5 diopters) in at least one eye. MAIN OUTCOME MEASURE: Anisometropia. RESULTS: The prevalence rates of anisometropia, in terms of spherical equivalent (SE) difference of at least 1.5 diopters and 2.0 diopters were 1.57% (95% confidence interval [CI]: 1.1, 2.2) and 1.01% (95% CI: 0.6, 1.6), respectively. The prevalence rate of anisometropia (at least 2.0 diopters) among the children with at least one myopic eye was 2.4% (95% CI: 1.4, 3.8), whereas in those without any myopic eyes, the prevalence rate was only 0.2% (95% CI: 0.06, 0.8). The spherical equivalent difference between the right and left eyes was positively correlated with the difference in axial lengths (P <.001). The difference in corneal refractive power is not statistically different between the anisometropic and the nonanisometropic children. CONCLUSIONS: The anisometropia prevalence rate in a childhood population with a relatively high prevalence of myopia was reported. The origin of the anisometropia is axial, and these results suggest that the differential rate of elongation between the two eyes of nonmyopic subjects results in anisometropia.

Anisometropia↗
PubMed2004

Optic disk and retinal characteristics in myopic children.

PURPOSE: To examine the baseline retina and optic disk characteristics in children. DESIGN: Cross-sectional study. METHODS: Subjects included 679 subjects with myopia and 100 with emmetropia, aged 8 to 13 years. Digital retina imaging and cycloplegic autorefraction were conducted to look for signs of retinal degeneration. Other outcome measures were vertical and horizontal cup-to-disk (CD) ratios, CD area ratio, parapapillary atrophy (PPA)-to-disk area ratio, and disk rotational angle. RESULTS: The prevalence of lattice degeneration was 0.15% (95% confidence interval: 0.008-1.00). No other retinal abnormalities were detected. Increased PPA-to-disk area ratio was associated with increased axial length, increased myopia severity, and increasing age. Cup-to-disk ratios were smaller in subjects with myopia compared with those with emmetropia, but this did not correlate with severity of myopia. In subjects with severe myopia, the upper pole of the disk was rotated away from the fovea but to lesser extent than in those with emmetropia or milder myopia. CONCLUSIONS: Apart from PPA, degenerative features were not commonly found in this study.

Adolescent↗
PubMed2004

Deep anterior lamellar keratoplasty in a patient with descemetocele following gonococcal keratitis.

PURPOSE: To describe the presentation and subsequent management of a case of severe gonococcal keratitis in a young man. DESIGN: Case report. METHODS: A young man presented with severe gonococcal keratitis. Topical and systemic antibiotics were given with no improvement, and progressive corneal melting was noted. Deep lamellar keratoplasty was performed. The clarity and state of corneal graft and the postoperative visual acuity were noted. RESULTS: At 6 months posttreatment, the graft was clear, and no recurrence of infection was noticed. Corrected visual acuity was 20/25. CONCLUSION: Deep lamellar keratoplasty should be considered as a therapeutic option in patients with severe gonococcal keratitis that does not respond to antibiotic treatment.

Acute Disease↗
PubMed2004

Screening for myopia and refractive errors using LogMAR visual acuity by optometrists and a simplified visual acuity chart by nurses.

PURPOSE: To compare the sensitivity and specificity of a widespread method of screening for refractive errors in Singapore schoolchildren using a simplified acuity screening chart with a more rigorous method using the Early Treatment Diabetic Retinopathy Study (ETDRS) chart. A secondary aim is to estimate the best cutoff values for the detection of refractive errors using these two methods. METHODS: This is a population-based study, involving 1779 schoolchildren from three schools in Singapore. Logarithm of the minimum angle of resolution (logMAR) visual acuity was recorded using a modified Bailey-Lovie chart by trained optometrists, and visual acuity measurement was also undertaken using a simplified 7-line visual acuity screening chart by school health nurses. The main outcome measures were the receiver-operating characteristics (ROC's) of logMAR and the simplified screening visual acuity to detect myopia or any refractive errors. The difference between measurements, simplified screening visual acuity--logMAR visual acuity, was calculated. RESULTS: The optimal threshold using the simplified screening visual acuity chart for the detection of myopia or any refractive error was 6/12 or worse. Using logMAR visual acuity, the most efficient threshold for the detection of myopia was 0.26, but this was 0.18 for the detection of any refractive error. The area under the ROC curves was significantly greater in the case of the logMAR visual acuity measurement compared with the simplified screening visual acuity measurement for the detection of myopia or any refractive errors. The 95% limits of agreement for the two methods (simplified screening--logMAR acuity) was -0.219 to +0.339. CONCLUSIONS: Bearing in mind that the visual acuity measurements were performed by two different groups of professionals, visual acuity screening using the ETDRS method appears to be more accurate than the simplified charts for the detection of myopia or any refractive errors in children.

Child↗
PubMed2004

Corneal thickness determination and correlates in Singaporean schoolchildren.

PURPOSE: To determine the central cornea thickness (CCT) in Singaporean children and to examine the possible relationship between intraocular pressure (IOP) and other biometric factors and CCT. METHODS: This was a cross-sectional study. The subjects (N=652) were obtained from the Singapore Cohort Study of the Risk Factors for Myopia (SCORM). The subjects' ages ranged from 9 to 11 years. There were 485 Chinese, 92 Malayan, and 75 Asian Indian children. Measurement procedures included air-puff tonometry, noncontact slit lamp optical pachymetry, cycloplegic autorefraction, and autokeratometry. RESULTS: The mean CCT was 543.6 +/- 32.0 microm. Chinese children had thicker corneas than Malayan or Indian children (P=0.002). The boys had thicker corneas than girls (P=0.011), but the mean difference was only 6.4 microm. There was high correlation of CCT (r=0.98) and IOP (r=0.88) between right and left eyes. IOP was correlated with CCT (r=0.45, P <0.001). In a multiple linear regression model, each millimeter of mercury of IOP was associated with a CCT difference of 5.90 microm (95% confidence interval [CI], 4.98-6.82). The radius of corneal curvature correlated with CCT (r=0.19, P <0.001). The following parameters were not significantly (P >0.05) associated with CCT: age, family income, father's education, axial length, and spherical equivalent. CONCLUSIONS: The mean CCT in Singaporean children aged 9 to 11 years was 543.6 microm and showed ethnic and gender variation. CCT affected measured IOP and correlated weakly with corneal curvature. Compared with data in adults, a change in CCT was associated with a greater difference in measured IOP.

Anthropometry↗
PubMed2004

Incidence and progression of astigmatism in Singaporean children.

PURPOSE: This study investigated the incidence and progression, as well as factors associated with changes in astigmatism in school children. METHODS: This was a prospective cohort study. Children 7 to 9 years of age, of Chinese, Malay, and Asian Indian ethnicity, were examined annually over a 4-year period. Cycloplegic autorefraction was performed. A questionnaire was used to evaluate risk factors for incidence and progression of astigmatism. RESULTS: The cumulative 3-year incidence rate of astigmatism was 33.6% (cylinder power of 0.5 D or worse) or 11.5% (cylinder power of 1.0 D or worse). Myopic children had a higher incidence rate of astigmatism than nonmyopes (P <0.001). The mean J0 change per year was 0.012 D (95% CI: 0.007-0.018), whereas J45 did not show a significant change each year (mean, 0.001 D per year). Chinese children had greater worsening of J0 per year (P <0.001). Girls also had significantly greater progression of J0 than did boys (P <0.001). Similarly, myopia at baseline (P <0.001) and the hours of computer use (P=0.049) were associated with a greater progression rate of J0. J0 tended to improve in children with compound hyperopic astigmatism, worsen in children with compound myopic astigmatism, and remain stable in mixed astigmatics. CONCLUSIONS: Although there was minimal progression of astigmatism in school age children (0.44-0.53 D) over this period of follow-up, incident cases of astigmatism (>1.0 D) were not uncommon. The progression rate of astigmatism was affected by the ethnicity, presence of myopia, axis, and subtype of astigmatism.

Astigmatism↗
PubMed2003

Repeatability of ocular blood flow pneumotonometry.

PURPOSE: To establish the repeatability of ocular blood flow (OBF) pneumotonometry and its agreement with Goldmann tonometry. DESIGN: Instrument evaluation study. PARTICIPANTS: Ten female healthcare professionals. METHODS: Intraocular pressure (IOP) was measured by one experienced ophthalmologist in both eyes of 10 healthy female subjects on 10 different occasions at the same time of day. The two methods were performed by alternate allocation, and laterality was chosen by random order. Repeatability coefficients and agreement plots were calculated by using the Bland-Altman method. MAIN OUTCOME MEASURES: Intraocular pressure repeatability coefficients. RESULTS: Mean IOPs were 15.6 mmHg (right) and 15.1 mmHg (left) by OBF pneumotonometry and 12.6 mmHg (right) and 12.4 mmHg (left) by Goldmann tonometry (P < 0.001). The repeatability coefficients were 7.06 (right) and 7.66 (left) for the OBF pneumotonometer and 4.81 (right) and 3.87 (left) for the Goldmann tonometer. With regard to agreement, the OBF pneumotonometer read significantly higher than did the Goldmann tonometer. The mean bias for the right eye was 2.92 (95% limits of agreement, -4.37 to 10.20), and for the left eye it was 2.68 (95% limits of agreement, -3.93 to 9.28). CONCLUSIONS: In our group of healthy females, the repeatability of the OBF pneumotonometer was worse than that of the Goldmann tonometer. This casts doubt on the value of the OBF pneumotonometer as a tool for measuring IOP. The agreement plots indicate that the OBF pneumotonometer may produce significant numbers of false-positive results in screening programs.

Adult↗
PubMed2003

Agreement between Scheimpflug photography and A-scan ultrasonography in anterior segment ocular measurements in children.

PURPOSE: The Scheimpflug principle, as a method of performing biometry on the anterior segment, has not been widely used in the clinical setting compared with ultrasonography. The purpose of this study was to examine the agreement in anterior chamber length and lens thickness measurements between A-scan ultrasonography and Scheimpflug photography. METHODS: A total of 252 children aged between 6 and 12 years participated in this study. All subjects had instillation of cycloplegic eye drops before measurement. Scheimpflug photography and A-scan ultrasonography were performed in this sequence on the same day. The anterior segment length on the Scheimpflug images was taken as the axial corneal thickness added to the anterior chamber depth. A-scan ultrasonography was performed by placing the probe on the corneas after topical anesthesia. Differences were obtained by subtracting the A-scan ultrasonography measurements from the Scheimpflug measurements. Data were analyzed using difference vs. mean plots. RESULTS: For anterior chamber length, the mean difference between the two methods was -0.03 +/- 0.16 mm, with 95% limits of agreement of -0.35 to +0.30 mm. However, Scheimpflug readings were greater than that of ultrasonography for smaller measurements, and the opposite was true for larger measurements. For lens thickness, the mean difference was -0.2 +/- 0.10 mm, with 95% limits of agreement of -0.40 to +0.00 mm. For the measurement of lens thickness, adding a correction factor of 0.2 mm to Scheimpflug's readings should replace that of A-scan readings reasonably well. CONCLUSIONS: The readings obtained from these two methods of biometry must be used with the knowledge of the instruments. We discuss the possible reasons for the difference in readings obtained using these two instruments.

Anterior Eye Segment↗
PubMed2002

Refractive error and monochromatic aberrations in Singaporean children.

Higher order optical aberrations were measured in 273 cyclopleged Singaporean school children using a Bausch and Lomb Zywave aberrometer, with 268 of these subjects also undergoing corneal topography measurements (Tomey TMS 2 system). Subjects with low myopia (> -3.00 to -0.50 D) showed slightly, but significantly, less positive levels of spherical aberration than other refractive error groups. Chinese subjects also showed significantly higher amounts of aberrations than Malay subjects, particularly for vertical coma, but also for horizontal coma and spherical aberration. Anterior corneal spherical aberration (calculated from topography) was significantly correlated with whole eye spherical aberration, but did not vary significantly with refractive error or racial background. Residual spherical aberration (i.e. of posterior cornea and crystalline lens) did vary significantly with refractive error and race. Our results do not provide any evidence for aberration-driven form-deprivation as a major mechanism of myopia development.

Child↗