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Luis Rueda

Publications and source records attributed to Luis Rueda.

3 recordsLinked to original sources

Stochastic automata-based estimators for adaptively compressing files with nonstationary distributions.

This correspondence shows that learning automata techniques, which have been useful in developing weak estimators, can be applied to data compression applications in which the data distributions are nonstationary. The adaptive coding scheme utilizes stochastic learning-based weak estimation techniques to adaptively update the probabilities of the source symbols, and this is done without resorting to either maximum likelihood, Bayesian, or sliding-window methods. The authors have incorporated the estimator in the adaptive Fano coding scheme and in an adaptive entropy-based scheme that "resembles" the well-known arithmetic coding. The empirical results obtained for both of these adaptive methods are obtained on real-life files that possess a fair degree of nonstationarity. From these results, it can be seen that the proposed schemes compress nearly 10% more than their respective adaptive methods that use maximum-likelihood estimator-based estimates.

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Spot detection and image segmentation in DNA microarray data.

Following the invention of microarrays in 1994, the development and applications of this technology have grown exponentially. The numerous applications of microarray technology include clinical diagnosis and treatment, drug design and discovery, tumour detection, and environmental health research. One of the key issues in the experimental approaches utilising microarrays is to extract quantitative information from the spots, which represent genes in a given experiment. For this process, the initial stages are important and they influence future steps in the analysis. Identifying the spots and separating the background from the foreground is a fundamental problem in DNA microarray data analysis. In this review, we present an overview of state-of-the-art methods for microarray image segmentation. We discuss the foundations of the circle-shaped approach, adaptive shape segmentation, histogram-based methods and the recently introduced clustering-based techniques. We analytically show that clustering-based techniques are equivalent to the one-dimensional, standard k-means clustering algorithm that utilises the Euclidean distance.

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A hill-climbing approach for automatic gridding of cDNA microarray images.

Image and statistical analysis are two important stages of cDNA microarrays. Of these, gridding is necessary to accurately identify the location of each spot while extracting spot intensities from the microarray images and automating this procedure permits high-throughput analysis. Due to the deficiencies of the equipment used to print the arrays, rotations, misalignments, high contamination with noise and artifacts, and the enormous amount of data generated, solving the gridding problem by means of an automatic system is not trivial. Existing techniques to solve the automatic grid segmentation problem cover only limited aspects of this challenging problem and require the user to specify the size of the spots, the number of rows and columns in the grid, and boundary conditions. In this paper, a hill-climbing automatic gridding and spot quantification technique is proposed which takes a microarray image (or a subgrid) as input and makes no assumptions about the size of the spots, rows, and columns in the grid. The proposed method is based on a hill-climbing approach that utilizes different objective functions. The method has been found to effectively detect the grids on microarray images drawn from databases from GEO and the Stanford genomic laboratories.

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