Biomedical subjects

Luzheng Liu

Publications and source records attributed to Luzheng Liu.

3 recordsLinked to original sources

PubMed2004

Assessment of lymphocyte-mediated cytotoxicity using flow cytometry.

Cytotoxic lymphocytes, including cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells, kill target cells by releasing granules containing perforin and granzymes, and/or via Fas-Fas ligand interactions. Both pathways lead to prompt activation within target cells of caspase cascades responsible for apoptosis induction and cell death. We have utilized cell-permeable fluorogenic caspase substrates and multiparameter flow cytometry to detect caspase activation in target cells, and applied these tools to quantify and visualize cytotoxic lymphocyte activities. This novel assay, referred to as the flow cytometric cytotoxicity (FCC) assay, is a nonradioactive single-cell-based assay that provides a more rapid, biologically informative, and sensitive approach to measure cytotoxic lymphocyte activity when compared to other assays such as the 51chromium (51Cr) release assay. In addition, the FCC assay can be used to study CTL-mediated killing of primary target cells of different cell lineages that are frequently not amenable to study by the 51Cr release assay. Furthermore, the FCC assay enables evaluation of the phenotype and fate of both target and effector cells, and as such, provides a useful new approach to illuminate the biology of cytotoxic lymphocytes.

Apoptosis↗

PubMed2002

Visualization and quantification of T cell-mediated cytotoxicity using cell-permeable fluorogenic caspase substrates.

We have developed a non-radioactive flow-cytometry assay to monitor and quantify the target-cell killing activities mediated by cytotoxic T lymphocytes (CTLs). This flow-cytometry CTL (FCC) assay is predicated on measurement of CTL-induced caspase activation in target cells through detection of the specific cleavage of fluorogenic caspase substrates. Here we show that this assay reliably detects antigen-specific CTL killing of target cells, and demonstrate that it provides a more sensitive, more informative and safer alternative to the standard 51Cr-release assay most often used to quantify CTL responses. The FCC assay can be used to study CTL-mediated killing of primary host target cells of different cell lineages, and enables the study of antigen-specific cellular immune responses in real time at the single-cell level. As such, the FCC assay can provide a valuable tool for studies of infectious disease pathogenesis and development of new vaccines and immunotherapies.

Animals↗

PubMed1999

A global learning algorithm for a RBF network.

This article presents a new learning algorithm for the construction and training of a RBF neural network. The algorithm is based on a global mechanism of parameter learning using a maximum likelihood classification approach. The resulting neurons in the RBF network partitions a multidimensional pattern space into a set of maximum-size hyper-ellipsoid subspaces in terms of the statistical distributions of the training samples. An important feature of the algorithm is that the learning process includes both the tasks of discovering a suitable network structure and of determining the connection weights. The entire network and its parameters are thought of evolved gradually in the learning process.

Journal Article↗