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Biomedical subjects

M A Brunelli

Publications and source records attributed to M A Brunelli.

At least 37 records · Page 2Linked to original sources
PubMed1977

Experimental and clinical investigations on stem cell take and colony formation.

First, lymphocyte transplantation into total body-irradiated rats is discussed. The effect on spleen colony formation caused by the transplantation of untreated lymphocytes, as well as of lymphocytes previously incubated with PHA, or with PHA plus L-asparaginase, or with lymphokines, was studied. Then the effect of the urinary colony-stimulating factor in vitro, and the in vitro feeder-layer activity of leucocytes on colony formation of human and mice bone marrow cells in haematological diseases is dealth with. The injection of rat lymphocytes previously incubated for 24 hours with PHA resulted in a higher number and a larger size of colonies in the spleen of the recipient rats. Lymphocytes preincubated with lymphokines gave rise to the formation of spleen colonies which were larger than those developing after the injection of untreated lymphocytes. When the lymphocytes were previously incubated with PHA plus L-asparaginase, PHA failed to stimulate colony formation in the spleen. The phenomenon is explained by assuming that PHA, as an aspecific stimulator of cell division, initiated the division of CFUs, thus the CFUs content of the preincubated samples increased, resulting in an increase in the number of colonies formed after the transplantation of lymphocytes pretreated with PHA. Another possible explanation is that CFUs division, or their spleen take is enhanced by the immunocompetent lymphocytes activated by PHA, either directly or via soluble mediators produced or released by immunocompetent lymphocytes such as lymphokines. The study of colony-forming cells and colony-stimulating activity in primary myelofibrosis (PM) showed an increase in the number of circulating CFUc in this condition, and an abnormal density of these cells reaching a peak below 1.062. The lowering of CSA in the first two peripheral blood gradient fractions agrees with the observation in the same fractions of a high percentage of CFUc at the expense of the CSC population. Thus, double cell population seems to exist in PM. One is greatly abnormal with a low specific density and high plating efficiency, whereas the other population is almost normal, showing a higher specific density and a lower plating efficiency.

Animals↗
PubMed1976

Colony forming activity, density distribution and morphological features of the stem cell population in primary myelofibrosis.

In four patients with untreated primary myelofibrosis, the peripheral-blood cell growth in agar culture (frequency of clusters and colonies) was studied. As cell sources, were used either the buffy coat or the fractions obtained from an albumin discontinuous density gradient, prepared according to Dicke's technique. Besides the agar culture, also morphological features of the blasts of the granulocytic cell series of the gradient fractions were investigated, in order to relate the above-mentioned cell parameters. The results, taken as a whole, pointed out: a) increase in circulating CFUc's (expressed as the number/10(5) nucleated cells) in the patients, when compared to the controls; b) high percentage of CFUc's with an abnormal specific density (below 1.062 in 3 out of 4 patients); c) presence in 3 out of 4 patients, of two populations of blasts: one showing low specific density, small size, high N/C rate, high plating efficiency and no nucleoli; the other with a higher specific density and low plating efficiency, showing the typical morphological features of the myeloblasts.

Adult↗
PubMed1976

A preliminary study on humoral control of granulopoiesis in primary myelofibrosis and chronic granulocytic leukaemia.

The extent of the urine Colony Stimulating Factor (CSF/u), as well as the Colony Stimulating Activity (CSA) of peripheral-white blood cells (WBC) were evaluated in two groups of patients with myeloproliferative disorders, at the disease onset. The first patient group deals with Primary Myelofibrosis (PM), whereas the second group deals with Chronic Granulocytic Leukaemia (CGL). The Stanley technique was used for the above-mentioned assay, and in each group of patients either human or murine bone-marrow cell-suspensions were cultivated. In all patients with PM, a low CSF/u was detected, while patients with CGL showed a high CSF/u, regardless whether Ph1-positive or not. CSA of WBC in the PM-group was normal. In contrast, a significant low CSA in the CGL-group was noted. This fact could be of some importance in differentiating the two disorders at their onset.

Animals↗
PubMed1975

Stimulating factors in abnormal haematologic conditions.

Part of the present knowledge, due to the in vitro assays of human normal and leukaemic granulocytopoiesis, is briefly reviewed, with special attention to the meaning of the leukaemic CFU-C, and to the possible pathogenetic role of the relationship of CFU-C with CSF, in normal vs. leukaemic conditions. It is suggested that the role of CSF level during aplasia following cytotoxic therapy could be worthwhile of further investigation. Data are reported on the CSF activity of peripheral blood leukocytes in smouldering leukaemia (low), and in marrow aplasia (high), and of chronic myeloid leukaemia spleen cells (low CSF). Preliminary observations on the CFU-C content of spleen and liver in chronic myeloid leukaemia indicate that the role of these organs should receive adequate consideration.

Bone Marrow Cells↗