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M A Burich

Publications and source records attributed to M A Burich.

3 recordsLinked to original sources
PubMed1986

Evaluation of a rapid technique for antibody elution.

This study evaluated a new rapid acid elution technique using citric acid. This technique was compared to the commonly used digitonin acid procedure. Twenty patient or donor antibodies with specificities in the Rhesus, Kell, Kidd, or Duffy systems were used to coat red cells. Eluates produced from these cells by the citric acid procedure were equivalent or greater in volume and potency to those produced by a digitonin acid method. The citric acid procedure required five to nine minutes to complete, while the digitonin acid procedure required 28 to 44 minutes. The citric acid technique is a rapid means of producing a potent eluate.

Journal Article↗
PubMed1986

Evaluation of rapid antibody dissociation techniques.

Two new antibody dissociation techniques, citric acid treatment and chloroquine diphosphate (CDP) treatment at 37 C, were evaluated for their usefulness in investigating the red cells of patients with a positive direct antiglobulin test (DAT). The techniques were compared to the ZZAP and CDP-22 C procedures for (a) removal of coating antibody prior to performing warm autoadsorptions, and (b) removal of antibody so antigen typings can be performed. Twenty patient or donor antibodies were used to sensitize red cells in vitro. Complete removal of antibody was obtained by the CDP-37 C treatment in 19 cases (95%), by ZZAP treatment in 17 cases (8 5%), by the citric acid method in 10 cases (50%), and by the CDP-22 C method in three cases (15%). The CDP-37 C treatment left all red cell antigens investigated intact, and the citric acid technique left all but Kell system antigens intact. In addition, patient red cells treated by the citric acid technique could be used to adsorb warm-reacting autoantibodies from autologous serum in 15 to 30 minutes at room temperature. Investigation of a positive DAT may be facilitated by using the citric acid and CDP-37 C treatments.

Journal Article↗