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Biomedical subjects

M A Flynn

Publications and source records attributed to M A Flynn.

10 recordsLinked to original sources
PubMed1979

Effect of dietary egg on human serum cholesterol and triglycerides.

One hundred sixteen male volunteers between the ages of 32 and 62 years (mean age 46) consumed two whole fresh eggs daily in their customary diets for 3 months and also eliminated eggs for 3 months before or after eating eggs. The men had had normal-range serum cholesterol and triglycerides for the past 7 years. Four-day food records kept by them in each experimental period were assessed for nutrient intake. A Latin square design allowed analyses for season and sequential effects on serum lipids. The serum cholesterol and triglyceride levels at the end of 6 months were compared with their initial levels on customary free choice diets as well as their levels after the first 3 months of study. No significant increase in mean serum cholesterol was found nor was there a significant association of dietary cholesterol intake with either serum cholesterol or triglyceride.

Adult↗
PubMed1978

Effects of high- and low-fiber diets on human feces.

Increased- and low-fiber diets composed of common hospital foods were tested for their ability to alter stool weight, frequency of defecation, transit time, and absorption efficiency. The diets were calculated to be isocaloric and similar in protein, fat, and carbohydrate. They were dissimilar in ash and fiber. No stimulants, secretagogues, food concentrates, or extracts were used on either diet. Six normal, healthy, male subjects consumed each constant diet for five to eight days. Diets and stool composites were analyzed for protein, fat, fiber, moisture, and ash. Carbohydrate and calories were calculated. Stool weight during ingestion of the low-fiber diet averaged 51 gm. per day; subjects had an average of one bowel movement every 33 hr.; and the mean transit rate was 48 hr. When subjects consumed the increased-fiber diet, values were 157 gm., 19h., and 12 hr., respectively. Measurements of fecal protein, fat, carbohydrate, and calories from the low-fiber diet were less than half those observed when the increased-fiber diet was consumed. By altering specific components of a normal diet, e.g., fiber, significant differences can be made in bowel habit and absorption efficiency.

Adult↗
PubMed1977

Gas-liquid chromatography of fecal neutral steriods.

A method is described for the analysis of fecal neutral steriods with a dual-column gas-liquid chromatography (GLC) system. After saponification of the fecal slurry, the neutral steroids were extracted with hexane. The GLC separation of the compounds and quantitation were achieved by simultaneous injection of the derivatized and derivatized aliquots of the extract onto dual colmuns under identical conditions. The neutral steroids of interest were than identified by matching the retention times with those of known standards, and identification was confirmed by use of an interfaced GLC high-resolution mass spectrometry system. The detection limit was 0.003 mg of steroid/g of fecal slurry. The pricision of the method is illustrated by a relative standard diviation of 2-10% and a recovery of neutral steroids from 73-96%. The method was applied to the determination of fecal neutral steroids in a "High protein diet in colon cancer study". A considerably larger level of coprostanone than of coprostanol was observed. Data on neutral steroids in fecal samples from subjects on different diets are the subject of a separate publication.

Cholestanes↗
PubMed1977

Effect of dietary egg on serum cholesterol and triglyceride of human males.

One hundred fourteen male volunteers (mean age 44.6 years) consumed one whole egg daily in their customary diets for 3 months. Their final serum cholesterol (SCHOL) and triglycerides (STG) levels were compared with their initial levels on customary free choice diets and also with their levels after a 3-month elimination of dietary whole eggs. All subjects had previously confirmed normal serum lipid levels and no history of heart disease. Four-day food records were kept during both experimental dietary periods. A Latin square design allowed analysis for seasonal effects on lipid levels. No significant change in mean SCHOL on either diet was found; there was a seasonal effect on mean STG. Significant linear associations of fat intake and of energy intake were found. There was no significant association of dietary cholesterol intake with either SCHOL or STG.

Adult↗
PubMed1977

Effect of diet on fecal nutrients.

Calculated and chemically analyzed nutrient values for three weighed constant diets were dissimilar for calcium, magnesium, protein, and fat, and similar for fiber and ash. Fecal nutrient concentrations of ten healthy men fed the same constant diets were quite variable. Feeding constant weighed diets of similar composition does not assure the same fecal residue in the large colon of human subjects.

Adult↗
PubMed1977

Effect of a high-beef diet on the fecal bacterial flora of humans.

Ten human volunteers completed a 4-month diet series consisting of 1 month each of a control diet, a meatless diet, a high-beef diet, and the same control diet. Fat and fiber contents were essentially the same in all four diets, but protein content was doubled during the high-beef diet. During the 4th week on each diet, three stool specimens collected from each volunteer were analyzed for chemical composition and content of facultative, aerobic, and anaerobic bacteria. The bacteriological data are presented in this paper. High beef protein consumption had little effect on the composition of the intestinal flora. There were no significant differences in total counts of facultative and aerobic or anaerobic organisms in the feces when volunteers were on meatless or high-beef diets. At the species level, when counts during the two control diets were comparable, in only three instances did the change from the meatless to a high-beef diet significantly influence the bacterial numbers. The ratio of mean counts of anaerobic to facultative and aerobic organisms was approximately 15:1 during the meatless diet and 34:1 during the high-meat diet. The data indicate that animal protein consumption has little effect on the fecal bacterial profile in humans.

Animals↗