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M A GORDON

Publications and source records attributed to M A GORDON.

At least 19 recordsLinked to original sources

APPLICATION OF FLUORESCENT-ANTIBODY PROCEDURES TO THE STUDY OF PATHOGENIC DEMATIACEOUS FUNGI.II. SEROLOGICAL RELATIONSHIPS OF THE GENUS FONSECAEA.

Gordon, Morris A. (New York State Department of Health, Albany), and Yousef Al-Doory. Application of fluorescent-antibody procedures to the study of pathogenic dematiaceous fungi. II. Serological relationships of the genus Fonsecaea. J. Bacteriol. 89:551-556. 1965.-The staining spectra of fluorescein-labeled antiglobulins to Fonsecaea compactum, F. dermatitidis, and F. pedrosoi were determined with respect to 71 strains of morphologically related and 21 miscellaneous molds (including so-called "black yeasts"). All three conjugates showed considerable reaction with saprophytic Cladosporium spp. F. dermatitidis appeared to be most closely related serologically to the genus Cladosporium; F. compactum conjugate was fairly specific; and the few non-specific reactions of diluted F. pedrosoi reagent were almost unexceptionally with species of Fonsecaea and Cladosporium. Most strains of F. pedrosoi were unstained by F. compactum conjugate, and there was very little relationship shown between either of these species and Phialophora verrucosa. Conjugates of two strains of P. verrucosa failed to react with any of the three species of Fonsecaea. The possible significance of these results for classification of the "black yeasts" and other dematiaceous molds is discussed.

Ascomycota↗

THE GENUS DERMATOPHILUS.

Gordon, M. A. (New York State Department of Health, Albany). The genus Dermatophilus. J. Bacteriol. 88:509-522. 1964.-Seventeen strains of Dermatophilus originating in skin lesions of cattle, sheep, horses, deer, and man were compared as to conditions for growth, colonial characteristics under varying conditions, microscopic morphology, and biochemical reactions. All grew well aerobically at 37 C and were facultatively anaerobic. They were morphologically similar in both gross and microscopic appearance, and most produced motile spores. Stable gray variants often appeared among the orange-yellow "wild-type" colonies. Acid without gas was produced consistently from glucose and fructose, and transitorily from galactose, but was produced from none of eight other carbohydrates except belatedly by some strains from maltose. Almost all strains hydrolyzed casein, most of them digested BCP milk with varying rapidity, and the majority liquefied gelatin, but there was considerable variation in this last property. Differences crossed both host and geographic lines. It is concluded that all isolates can be accommodated in the species D. congolensis Van Saceghem 1915, emend. 1916, 1934, with D. dermatonomus and D. pedis falling into synonymy.

Actinomycetales↗

APPLICATION OF FLUORESCENT-ANTIBODY PROCEDURES TO THE STUDY OF PATHOGENIC DEMATIACEOUS FUNGI.

Al-Doory, Yousef (Division of Laboratories and Research, New York State Department of Health, Albany) and Morris A. Gordon. Application of fluorescent-antibody procedures to the study of pathogenic dematiaceous fungi. I. Differentiation of Cladosporium carrionii and Cladosporium bantianum. J. Bacteriol. 86:332-338. 1963.-The well-known polymorphism and confusing similarity of cultures of pathogenic dematiaceous molds and other so-called "black yeasts" make individual species difficult to identify and even more perplexing to classify. Serological investigations of antigen relationships have proved of value in previous attempts to resolve these taxonomic problems, but thus far no fluorescent-antibody studies in this field have been reported. In the present investigations, fluorescein-labeled antisera to both Cladosporium carrionii and C. bantianum reacted with a number of dematiaceous species in addition to the homologous organisms, but selective dilution and absorption resulted in conjugates specific for each of these two species. Staining was confined to the periphery of both hyphae and spores, and age was a factor in the affinity of these structures for fluorescent antibody. There is evidence that antigen diffuses centrifugally from the hyphae during growth on agar media.

Ascomycota↗

MICROMORPHOLOGY OF DERMATOPHILUS CONGOLENSIS.

Gordon, Morris A. (Division of Laboratories and Research, New York State Department of Health, Albany), and Mercedes R. Edwards. Micromorphology of Dermatophilus congolensis. J. Bacteriol. 86:1101-1115. 1963.-As seen in electron micrographs of thin sections, Dermatophilus congolensis is a holocarpic actinomycete that fragments, after formation of septa in several planes, into Sarcina-like packets and then into individual cocci. Release of coccal forms from the filaments and packets is by dissolution of a capsular matrix, which is a product of degradation of the cell wall. The plasmalemma is a "unit membrane." Regularly occurring plasmalemmosomes ("onion bodies") of uniform structure are apparently related to septum formation. A typical bacterial nucleoid is seen in most sections, and ribosomes are scattered throughout the cytoplasm. Specimens for electron microscopy were prepared by a modification of Kellenberger's method.

Actinomycetales↗