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Biomedical subjects

M A Iakhno

Publications and source records attributed to M A Iakhno.

At least 19 recordsLinked to original sources
PubMed1977

[Transfection by integrated proviruses].

Three lines of continuous mouse L cells were compared: one of them contained only its endogenic oncornavirus, another was contaminated with SV5 virus, and a third was obtained from the second line chronically infected with vesicular stomatitis virus. Experiments with transfection showed that it was possible to recover SV5 virus from the two former cultures and vesicular stomatitis virus from the latter.

Animals↗
PubMedDate not supplied

[Effective methods of obtaining antisera to hemagglutinin H3].

Schedules for production of antisera to pure influenza A virus hemagglutinin (H3) in rats and rabbits are described. The antisera prepared in rats were shown to be better than rabbit sera as they had low levels of inhibitors readily eliminated by heating. The best sera were obtained by immunizations of rats into the tail. Both rat and rabbit antisera, in addition to the HI test, can be used satisfactorily in the immunodiffusion test and in radioimmunoassay.

Animals↗
PubMedDate not supplied

[The characteristics of influenza A/H1N1 viruses related to A/PR/8/34 isolated in the Mongolian People's Republic].

Investigations of the antigenic structure and genome of influenza A (H1N1) viruses isolated in the Mongolian People's Republic in 1982, 1983, 1986 and 1987 from children with acute respiratory diseases using monoclonal antibodies and nucleotide sequencing revealed 4 strains identical to the prototype strain A/PR/8/34 (H1N1), variant M. Sinai, and 8 strains closely similar to the epidemic strain A/USSR/90/77 (H1N1) in the antigenic structure and A/Leningrad/54 (H1N1) in the primary structure of HA.

Antigens, Viral↗
PubMedDate not supplied

[Identification of a hemadsorbing agent discovered in uninfected mouse L cell cultures and also the same cultures chronically infected with Sindbis virus].

Electron microscope examinations of continuous lines of mouse L cells, both uninfected (L-init) and chronically infected with Sindbis virus (L-SV) revealed accumulations of ribonucleoprotein strands and virions corresponding by their parameters to paramyxoviruses in the cytoplasms of the cells. Further studies showed L-init and L-SV cell lines to have a manifest hemadsorption effect which could be completely inhibited by antiserum to parainfluenza SV5 virus. Immunofluorescence procedures detected intensive fluorescence in the cytoplasm of these cells which was observed only after treatment of the cells with antiserum to SV5 virus. In response to inoculation of cell homogenates of continuous L-init and L-SV cultures guinea pigs developed antihemagglutinating antibody to simian parainfluenza SV5 virus. On the basis of these results it may be assumed that virus-specific structures and viruses identical by their parameters to paramyxoviruses observed in electron microscope examinations of continuous mouse L-init and L-SV cells are simian parainfluenza SV5 virus.

Animals↗
PubMedDate not supplied

[The antigenic and biological properties of influenza A (H1N1) viruses isolated in the USSR and the Czechoslovak Federal Republic in 1988-1989].

A comparative study of influenza A (H1N1) virus strains isolated in one epidemic season (1988-1989) in neighbouring countries was carried out. Using polyclonal and monoclonal antibodies, some of the isolates were shown to have different antigenic and biological properties which probably determined the different intensity of the epidemic situation in the USSR and CzSFR in 1988-1989.

Antigens, Viral↗
PubMedDate not supplied

[Influenza viruses studied by fluorescent immunoassay with temporary resolution].

Monoclonal antibodies, epidemic strains of influenza A and B viruses, conjugates were studied by fluorescence immunoassay with temporary resolution using equipment of different companies. Differences and specificity of monoclonal antibodies to influenza A and B viruses were demonstrated. The highest sensitivity of the method with the test system used was determined, being 20 ng/ml of viral protein. The method was shown to be useful for influenza virus detection both in solutions containing purified virions and in virus-containing allantoic preparations. It was established that virological studies could be carried out using conjugates and knock-down microplates of the national make alongside with foreign ones.

Antibodies, Monoclonal↗
PubMedDate not supplied

[Antigenic specificity of the neuraminidases of epidemic strains of influenza A/H3N2 viruses circulating in the USSR 1983-1985].

The antigenic properties of neuraminidase of the epidemic strains of 1983-1985 were studied by means of lectin test and thiobarbituric method using polyclonal sera and monoclonal antibody. Populations of the viruses with respect to antigenic relationships of neuraminidase were shown to be heterogeneous. A considerable portion of the strains occurring in 1985 underwent antigenic drift from the reference A/Philippines/2/82 strain. The epidemic novelty of some strains was due to changes in only one supercapsid protein-neuraminidase.

Antibodies, Monoclonal↗
PubMedDate not supplied

[Analysis of the antigenic specificity of the neuraminidases of influenza B viruses isolated in different years].

The antigenic structure of neuraminidases of influenza B viruses isolated in 1940-1984 was studied. Lectin test has first been employed for analysis of influenza B virus neuraminidase. The antigenic composition of neuraminidases of these viruses was shown to be different. The strains isolated in the epidemic of 1983-1984 underwent further drift of the neuraminidase component and represent a heterogeneous group in this respect. The use of monospecific antiserum to purified neuraminidase helped specify antigenic relationships of this protein.

Antigens, Viral↗
PubMedDate not supplied

[Antigenic and biological characteristics of strains of the influenza A (H3N2) virus, similar to virus A/Hong Kong/68, isolated from children in the USSR in 1986].

Influenza A viruses (H3N2) isolated in the USSR, 1986, from children were antigenically similar to the prototype A/Hong Kong/1/68 strain. Alongside with the similarity with the latter virus, the new isolates had a number of distinctive features both in the antigenic specificity of hemagglutinin and in the structure of the polymerase complex proteins. All the foregoing allows to exclude laboratory contamination and suggests the possibility of circulation of such viruses among children.

Antibodies, Monoclonal↗
PubMedDate not supplied

[Antigenic drift of epidemic strains of influenza A virus (H3N2) in 1985].

The study included 230 strains of influenza A (H3N2) virus isolated in the epidemic of 1985. A high degree of heterogeneity of the virus population was established with polyclonal sera, monoclonal antibodies and the method of kRNA-bRNA hybridization for the determination of the genome composition. Among the strains of one epidemic (1985) three antigenically heterogenous groups of strains were detected similar with reference A/Philippines/2/82, A/Ken/1/84, and A/Mississippi/1/85 strains. It was shown that in the process of antigenic drift in some strains there occurred a reversion of the antigenic determinants similar to those of previously circulating epidemic A/Texas/1/77 strain.

Animals↗
PubMedDate not supplied

[A comparative analysis of epidemic strains of the influenza A virus (H1N1), isolated in the USSR and Czechoslovakia in 1986].

A comparative study of antigenic and molecular-biological properties of epidemic influenza virus strains isolated in the USSR and CSSR in the autumn-winter, 1986-1987 was carried out and showed that the epidemics in both countries were due to influenza A viruses of H1N1 subtype, antigenically related to the reference A/Taiwan/1/86 and A/Singapore/6/86 strains, which was confirmed by electrophoretic studies. The strains were found to vary in sensitivity to heating and to the inhibitors.

Czechoslovakia↗