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Biomedical subjects

M A Kilmore

Publications and source records attributed to M A Kilmore.

13 recordsLinked to original sources

Efficacy of high-velocity low-amplitude manipulative technique in subjects with low-back pain during menstrual cramping.

Previous studies have shown that dysmenorrhea produces low-back pain and an electromyographic (EMG) pattern typical of trauma-induced low-back pain. To determine the effects of high-velocity low-amplitude osteopathic manipulative treatment (OMT) on this type of low-back pain, 12 dysmenorrheic subjects were assigned to a group receiving OMT or to a group not receiving OMT (or both). Eight subjects participated in both groups, the other four being equally distributed between groups. Osteopathic manipulative treatment significantly decreased EMG activity during extension of the lumbar spinae erector muscles and abolished the spontaneous EMG activity. These EMG changes coincided with the patient's report of alleviated low-back pain and menstrual cramping. Osteopathic manipulative treatment did not change the creatinine kinase, lactate dehydrogenase or lactate-dehydrogenase isoenzyme activity, or myoglobin concentration.

Adult↗

Effect of disulfiram on canine liver aldehyde dehydrogenase activity: in vivo inactivation in a nonrodent animal model.

Dogs were used as nonrodent animal models to study the in vivo effects of disulfiram on hepatic aldehyde dehydrogenase (ALDH) activity. Dogs were treated with disulfiram either intraperitoneally or orally (100 mg/kg/day for 2 days followed by 40 mg/kg/day for 3 days). Liver biopsies from control and treated animals were fractionated by differential centrifugation and the subcellular fractions were analyzed for ALDH activity. Significantly less activity was observed in cell homogenates from treated animals (20-50% of control activity per gram of liver). The majority of loss in activity was accounted for by a decrease in ALDH activity in the soluble fraction of the cell (12-30% of control activity) and in the mitochondrial fraction (23-30% of control activity). Activities at both high (5 mM) and low (50 microM) acetaldehyde concentrations were affected. The subcellular distribution of ALDH activity and in vivo inhibition by disulfiram in dogs is different from that reported for rats.

Aldehyde Dehydrogenase↗