Methods and methodological approaches to studies of isolated neurons of brain from adult animals (Lymnaea stagnalis) in tissue culture.
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Animals↗
Biomedical subjects
M A Kostenko
Publications and source records attributed to M A Kostenko.
Morphological differentiation of NIE-115 mouse neuroblastoma cells.
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Animals↗
[Cryopreserved neurons of a mollusc are capable of morphological differentiation in culture in vitro].
It was shown in culture in vitro that neurons isolated from the cryopreserved brain of adult molluscs Lymnaea stagnalis L. retain viability. Isolated brains were frozen in liquid nitrogen vapors at a rate of 400-500 degrees C/min in the presence of 2 M dimethylsulfoxide. The samples were then plunged into liquid nitrogen and stored from 1 month to 2 years. Upon thawing and removing the cryoprotectant, the neurons were isolated from the brain and then introduced into a cellular culture in vitro. It was shown that the thawed neurons were capable of regenerating new nerve processes similar to those formed by unfrozen neurons in the control.
Animals↗
[Needle holder for round nontraumatic needles].
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Needles↗
[An electrosurgical device for coagulating biological tissue and arresting parenchymatous hemorrhage].
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Electrocoagulation↗
[Ionic regulation of neuronal differentiation and regeneration in culture].
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Animals↗