PubMed Health⌕ Search

Biomedical subjects

M A Kuznetsova

Publications and source records attributed to M A Kuznetsova.

At least 19 recordsLinked to original sources
PubMed2005

Perfluoroarylazide derivatives of 2'-O-modified oligoribonucleotides: efficient reagents for RNA photomodification.

Site-specific photomodification of the 5'-terminal fragment of MDR1 mRNA by perfluoroarylazide derivatives of 2'-O-modified (2'-O-methyl or 2'-O-tetrahydropyranyl) oligoribo- and oligodeoxyribonucleotides was investigated. The conjugates built of 2'-O-modified oligoribonucleotides demonstrate beneficial features compared with their deoxyribo analogs: the extent of RNA modification by 2'-O-modified oligoribonucleotides and oligodeoxyribonucleotide conjugates was 40- 50% and 20%, respectively.

Azides↗
PubMed2004

Two-component 10-23 DNA enzymes.

A new strategy for engineering of catalytic two-component constructions based on 10-23 DNAzyme was proposed. The using of a combination of shortened DNAzyme with 2'-O-methyl oligomers as effectors significantly increased the catalytic activity of this DNAzyme.

Base Sequence↗
PubMed1996

[Measurement of lead and cadmium in urine and blood by the inversion volta amperometry using SVA-IBM analyzer].

The authors suggest a method evaluating lead and cadmium in human urine by means of new Russian device SVA-IBM (CBA-IBM). Inversion volta amperometry forms a basis of the method that is quite sensitive, precise and selective. Substances normal for mineralized urine and blood do not interfere with the evaluation. The method is recommended for evaluation of lead and cadmium for clinical, hygienic, toxicological purposes. Unlike polarographic method, the technique requires no metallic mercury and highly pure inert gas.

Cadmium↗
PubMed1991

The genetic aspects of influenza virus filamentous particle formation.

We analysed the genetic content of reassortants between parent viruses differing in their ability to form filaments. The results suggest that primarily HA, M, and NP genes are involved in the control of the filament forming ability. A lower buoyant density of the filamentous forms as compared to spherical particles allowed us to obtain a sufficiently pure population of filaments. A difference in the UV-inactivation kinetics between filaments and spherical virions suggests that the infectious filamentous forms are probably represented by multigenomic particles or partial heterozygotes.

Animals↗
PubMed1983

[Combined therapy of extra-abdominal desmoids].

Basing on a comparative study of the results of combined treatment (irradiation + operation) and surgery alone provided to patients with extra-abdominal desmoid (ED) the authors reach a conclusion that preoperative gamma-beam therapy can be regarded as an important means of recurrence prevention. In incomplete resection of tumors the treatment should be supplemented by postoperative irradiation, because in radiotherapy alone cure can be achieved with high summary doses only. It is still difficult to judge of the advantages of irradiation of ED under the conditions of hyperbaric oxygenation by the frequency of recurrences.

Adult↗
PubMed1981

[Effect of artificial gravitation during space flight on motor habit retention in white rats].

The recovery of a goal-reaching habit (food reaching) was studied in albino rats in a maze after space exposure on biosatellite "Kosmos-936". Animals which were exposed to artificial gravitation during the flight, restored the habit slightly worse than those not centrifuged. The tendency toward worsening is clearly pronounced, although the small number of animals and small changes (which to a considerable degree was due to relatively simple tasks) seem to produce insignificant differences at this stage of the study. The absence of positive effect of artificial gravitation might be connected with a negative effect of angular speeds during centrifugation.

Animals↗
PubMedDate not supplied

[Selection of antigenic variants of the influenza virus on the cells of different hosts].

Antigenic differences were found in influenza B virus variants isolated and propagated in different systems: chick embryos (E variants) and MDCK cell culture (M variants). The antigenic differences in M and E variants were detected in HI tests with polyclonal mouse sera and monoclonal antibodies as well as in biological neutralization tests in chick embryos and MDCK cell culture, and confirmed when M and E variants were used as antigens for antibody detection in human sera. By protein mobility in PAGE, M and E variants did not differ from each other and were also identical with the reference B/Victoria/87 strain.

Animals↗
PubMedDate not supplied

[The safety of the preparation viroden for vertebrate animals].

Toxicity-pathogenicity test of viroden, a new preparation, and its acting agent--a mosquito densonucleosis virus (MDV) has been carried out on warm-blooded animals. It is shown that the preparation is not toxic for laboratory animals (white common mice, rats, guinea pigs, rabbits), chicken embryos and cell cultures of warm-blooded animals. The MDV is not adapted to a warm-blooded organism with different ways of introduction and in passages. Using electron and luminescent microscopy, serological reactions, specific test systems and a biological test for sensitive insects no explicit or latent infection was found in animals, chicken embryos and cell cultures of vertebrates with primary infection and in passages. Sensibilized animals shown an immunological rearrangement of the organism proceeding by the retarded hypersensitivity type.

Animals↗