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Biomedical subjects

M A Srinivasan

Publications and source records attributed to M A Srinivasan.

At least 19 recordsLinked to original sources
PubMed1999

Simulation of tissue cutting and bleeding for laparoscopic surgery using auxiliary surfaces.

Realistic simulation of tissue cutting and bleeding is important components of a surgical simulator that are addressed in this study. Surgeons use a number of instruments to perform incision and dissection of tissues during minimally invasive surgery. For example, a coagulating hook is used to tear and spread the tissue that surrounds organs and scissors are used to dissect the cystic duct during laparoscopic cholecystectomy. During the execution of these procedures, bleeding may occur and blood flows over the tissue surfaces. We have developed computationally fast algorithms to display (1) tissue cutting and (2) bleeding in virtual environments with applications to laparoscopic surgery. Cutting through soft tissue generates an infinitesimally thin slit until the sides of the surface are separated from each other. Simulation of an incision through tissue surface is modeled in three steps: first, the collisions between the instrument and the tissue surface are detected as the simulated cutting tool passes through. Then, the vertices along the cutting path are duplicated. Finally, a simple elastic tissue model is used to separate the vertices from each other to reveal the cut. Accurate simulation of bleeding is a challenging problem because of the complexities of the circulatory system and the physics of viscous fluid flow. There are several fluid flow models described in the literature, but most of them are computationally slow and do not specifically address the problem of blood flowing over soft tissues. We have reviewed the existing models, and have adapted them to our specific task. The key characteristics of our blood flow model are a visually realistic display and real-time computational performance. To display bleeding in virtual environments, we developed a surface flow algorithm. This method is based on a simplified form of the Navier-Stokes equations governing viscous fluid flow. The simplification of these partial differential equations results in a wave equation that can be solved efficiently, in real-time, with finite difference techniques. The solution describes the flow of blood over the polyhedral surfaces representing the anatomical structures and is displayed as a continuous polyhedral surface drawn over the anatomy.

Algorithms↗
PubMed1998

Encoding of shape and orientation of objects indented into the monkey fingerpad by populations of slowly and rapidly adapting mechanoreceptors.

The peripheral neural representation of object shape and orientation was studied by recording the responses of a spatially distributed population of rapidly and slowly adapting type I mechanoreceptors (RAs and SAs, respectively) to objects of different shapes and orientations indented at a fixed location on the fingerpad of the anesthetized monkey. The toroidal objects had a radius of 5 mm on the major axis, and 1, 3, or 5 mm on the minor axis. Each object was indented into the fingerpad for 4 s at orientations of 0, 45, 90, and 135 degrees using a contact force of 15 gwt. Estimations of the population responses (PRs) were constructed by combining the responses of 91 SA and 97 RA single afferents at discrete times during the indentation. The PR was composed of the neural discharge rates (z coordinate) plotted at x and y coordinates of the most sensitive spot of the receptive field. The shapes of the PRs were related to the shapes of the objects by fitting the PRs with Gaussian surfaces. The orientations of the PRs were determined from weighted principal component analyses. The SA PR encoded both the orientation and shape of the objects, whereas the RA PR did neither. The SA PR orientation was biased toward the long axis of the finger. The RA PR encoded orientation only for the object with the highest curvature but did so ambiguously. Only the SA PR was well fit by a Gaussian surface. The shape of the object was discriminated by the SA PR within the first 500 ms of contact, and the form of the SA PR remained constant during the subsequent 3.5 s. This was manifested by constant widths of the PR along the major and minor axes despite a peak response that decreased from its maximum at 200 ms to an asymptotic value starting at 1 s. Thus the shape and orientation of each object were coded by the shape and orientation of the SA PR.

Adaptation, Physiological↗
PubMed1998

Raised object on a planar surface stroked across the fingerpad: responses of cutaneous mechanoreceptors to shape and orientation.

The representations of orientation and shape were studied in the responses of cutaneous mechanoreceptors to an isolated, raised object on a planar surface stroked across the fingerpad. The objects were the top portions of a sphere with a 5-mm radius, and two toroids each with a radius of 5 mm along one axis and differing radii of 1 or 3 mm along the orthogonal axis. The velocity and direction of stroking were fixed while the orientation of the object in the horizontal plane was varied. Each object was stroked along a series of laterally shifted, parallel, linear trajectories over the receptive fields of slowly adapting, type I (SA), and rapidly adapting, type I (RA) mechanoreceptive afferents innervating the fingerpad of the monkey. "Spatial event plots" (SEPs) of the occurrence of action potentials, as a function of the location of each object on the receptive field, were interpreted as the responses of a spatially distributed population of fibers. That portion of the plot evoked by the curved object (the SEPc) provided a representation of the shape and orientation of the two-dimensional outline of the object in the horizontal plane in contact with the skin. For both SAs and RAs, the major vector of the SEPc, obtained by a principal components analysis, was linearly related to the physical orientation of the major axis of each toroid. The spatial distribution of discharge rates [spatial rate surface profiles (SRSs), after plotting mean instantaneous frequency versus spatial locus within the SEPc] represented object shape in a third dimension, normal to the skin surface. The shape of the SA SRSs, well fitted by Gaussian equations, better represented object shape than that of the RA SRSs. A cross-sectional profile along the minor axis [spatial rate profile (SRP)] was approximately triangular for SAs. After normalization for differences in peak height, the falling slopes of the SA SRPs increased, and the base widths decreased with curvature of the object's minor axis. These curvature-related differences in slopes and widths were invariant with changes in object orientation. It is hypothesized that circularity in object shape is coded by the constancy of slopes of SA SRPs between peak and base and that the constancy of differences in the widths and falling slopes evoked by different raised objects encodes, respectively, the differences in their sizes and shapes regardless of differences in their orientation on the skin.

Action Potentials↗
PubMed1998

Force interactions in laparoscopic simulations: haptic rendering of soft tissues.

Research in the area of computer assisted surgery and surgical simulation has mainly focused on developing 3D geometrical models of the human body from 2D medical images, visualization of internal structures for educational and preoperative surgical planning purposes, and graphical display of soft tissue behavior in real time. Conveying to the surgeon the touch and force sensations with the use of haptic interfaces has not been investigated in detail. We have developed a set of haptic rendering algorithms for simulating "surgical instrument--soft tissue" interactions. Although the focus of the study is the development of algorithms for simulation of laparoscopic procedures, the developed techniques are also useful in simulating other medical procedures involving touch and feel of soft tissues. The proposed force-reflecting soft tissue models are in various fidelities and have been developed to simulate the behavior of elastically deformable objects in virtual environments. The developed algorithms deal directly with geometry of anatomical organs, surface and compliance characteristics of tissues, and the estimation of appropriate reaction forces to convey to the user a feeling of touch and force sensations.

Algorithms↗
PubMed1996

An investigation of the mechanics of tactile sense using two-dimensional models of the primate fingertip.

Tactile information about an object in contact with the skin surface is contained in the spatio-temporal load distribution on the skin, the corresponding stresses and strains at mechanosensitive receptor locations within the skin, and the associated pattern of electrical impulses produced by the receptor population. At present, although the responses of the receptors to known stimuli can be recorded, no experimental techniques exist to observe either the load distribution on the skin or the corresponding stress-state at the receptor locations. In this paper, the role of mechanics in the neural coding of tactile information is investigated using simple models of the primate fingertip. Four models that range in geometry from a semi-infinite medium to a cylindrical finger with a rigid bone, and composed of linear elastic media, are analyzed under plane strain conditions using the finite element method. The results show that the model geometry has a significant influence on the surface load distribution as well as the subsurface stress and strain fields for a given mechanical stimulus. The elastic medium acts like a spatial low pass filter with the property that deeper the receptor location, the more blurred the tactile information. None of the models predicted the experimentally observed surface deflection profiles under line loads as closely as a simple heterogeneous waterbed model that treated the fingerpad as a membrane enclosing an incompressible fluid (Srinivasan, 1989). This waterbed model, however, predicted a uniform state of stress inside the fingertip and thus failed to explain the spatial variations observed in the neural response. For the cylindrical model indented by rectangular gratings, the maximum compressive strain and strain energy density at typical receptor locations emerged as the two strain measures that were directly related to the electrophysiologically recorded response rate of slowly adapting type I (SAI) mechanoreceptors. Strain energy density is a better candidate to be the relevant stimulus for SAIs, since it is a scalar that is invariant with respect to receptor orientations and is a direct measure of the distortion of the receptor caused by the loads imposed on the skin.

Algorithms↗
PubMed1996

Neural encoding of shape: responses of cutaneous mechanoreceptors to a wavy surface stroked across the monkey fingerpad.

1. The role of cutaneous mechanoreceptors in the tactile perception of shape was investigated. Objects whose surfaces were shaped as a pattern of smooth, alternating convex and concave cylindrical surfaces of differing radii of curvature were constructed such that there were no discontinuities in the slope of the surface. These "wavy surfaces" were stroked across the fingerpad of the anesthetized monkey and electrophysiological responses of slowly adapting type I mechanoreceptive afferents (SAs) and rapidly adapting type I mechanoreceptive afferents (RAs) were recorded. 2. For both SAs and RAs, each convexity indenting the skin evoked a burst of impulses and each concavity of the same curvature that followed elicited a pause in response. "Spatial event plots" (SEPs) of the occurrence of action potentials as a function of the location of the object on the receptive field were obtained and interpreted as the responses of a spatially distributed population of fibers. With increasing magnitude of curvature (equivalently, decreasing radius of curvature) of convexity, the mean width of the burst in the SEPs for each fiber type (representing the width of a region of skin containing active fibers) decreased and the mean discharge rate during the burst increased. Over a range of velocities of stroking from 1 to 40 mm/s, the number of RAs activated increased with velocity, whereas SAs were active at all velocities. For both SAs and RAs, the burst rates increased with velocity, whereas the widths of the bursts and pauses remained approximately invariant. Thus the spatial measures of burst or pause width provide a robust representation of the size of a feature on the object surface. 3. For a given velocity of stroking, the spatially distributed pattern of averaged discharge rates (spatial rate profile, SRP) provided a representation of the shape of the wavy surface. The distance between neighboring peaks in the SRP for individual RAs and SAs was approximately the same as the distance between the peaks of the wavy surface. The averaged SRP for a population of SAs provided a better representation of shape than that for RAs. Whereas active regions in the SEP can be isomorphic to the two dimensional form of the stimulus "footprint" in contact with the skin surface, the SRP, which in addition encodes the features of the stimulus in the third dimension normal to the skin surface, is not isomorphic to the stimulus shape. 4. When the sizes as well as the shapes of objects are varied, it is hypothesized that a central processing mechanism extracts the invariant property of shape from the slopes of the rising and falling phases of an SRP that has been normalized for overall differences in discharge rates. These differences would be expected to occur with variations in the parameters of stimulation such as compressional force, stroke trajectory, and stroke velocity. It was shown that a common feature of the mean SRP for SAs evoked by each wavy surface convexity, regardless of its radius, was the constancy of the slope from the base to the peak and from the peak to the base. Thus a possible code for the constant curvature of a cylinder is the constancy of the slopes along the rising and declining phases of the triangular-shaped spatial response profile evoked in the SA population by the cylindrical convexity.

Animals↗
PubMed1995

Tactual discrimination of softness.

1. We investigated the ability of humans to tactually discriminate the softness of objects, using novel elastic objects with deformable and rigid surfaces. For objects with deformable surfaces, we cast transparent rubber specimens with variable compliances. For objects with rigid surfaces ("spring cells") we fabricated telescoping hollow cylinders with the inner cylinder supported by several springs. To measure the human discriminability and to isolate the associated information-processing mechanisms, we performed psychophysical experiments under three conditions: 1) active touch with the normal finger, where both tactile and kinesthetic information was available to the subject: 2) active touch with local cutaneous anesthesia, so that only kinesthetic information was available; and 3) passive touch, where a computer-controlled mechanical stimulator brought down the compliant specimens onto the passive fingerpad of the subject, who therefore had only tactile information. 2. We first characterized the mechanical behavior of the human fingerpad and the test objects by determining the relationship between the depth and force of indentation during constant-velocity indentations by a rigid probe. The fingerpad exhibited a pronounced nonlinear behavior in the indentation depth versus force trace such that compliance, as indicated by the local slope of the trace, decreased with increases in indentation depth. The traces for all the rubber specimens were approximately linear, indicating a constant but distinct value of compliance for each specimen. The fingerpad was more compliant than each of the rubber specimens. 3. All the human subjects showed excellent softness discriminability in ranking the rubber specimens by active touch, and the subjective perception of softness correlated one-to-one with the objectively measured compliance. The ability of subjects to discriminate the compliance of spring cells was consistently poorer compared with that of the rubber specimens. 4. For pairwise discrimination of a selected set of rubber specimens, kinesthetic information alone was insufficient. However, tactile information alone was sufficient, even when the velocities and forces of specimen application were randomized. In contrast, for discriminating pairs of spring cells, tactile information alone was insufficient, and both tactile and kinesthetic information were found to be necessary. 5. The differences in the sufficiency of tactile information for the discrimination of the two types of objects can be explained by the mechanics of contact of the fingerpad and its effect on tactile information. For objects with deformable surfaces, the spatial pressure distribution within the contact region depends on both the force applied and the specimen compliance.(ABSTRACT TRUNCATED AT 250 WORDS)

Anesthesia, Local↗
PubMed1995

Manual discrimination of compliance using active pinch grasp: the roles of force and work cues.

In these experiments, two plates were grasped between the thumb and the index finger and squeezed together along a linear track. The force resisting the squeeze, produced by an electromechanical system under computer control, was programmed to be either constant (in the case of the force discrimination experiments) or linearly increasing (in the case of the compliance discrimination experiments) over the squeezing displacement. After completing a set of basic psychophysical experiments on compliance resolution (Experiment 1), we performed further experiments to investigate whether work and/or terminal-force cues played a role in compliance discrimination. In Experiment 2, compliance and force discrimination experiments were conducted with a roving-displacement paradigm to dissociate work cues (and terminal-force cues for the compliance experiments) from compliance and force cues, respectively. The effect of trial-by-trial feedback on response strategy was also investigated. In Experiment 3, compliance discrimination experiments were conducted with work cues totally eliminated and terminal-force cues greatly reduced. Our results suggest that people tend to use mechanical work and force cues for compliance discrimination. When work and terminal-force cues were dissociated from compliance cues, compliance resolution was poor (22%) relative to force and length resolution. When work cues were totally eliminated, performance could be predicted from terminal-force cues. A parsimonious description of all data from the compliance experiments is that subjects discriminated compliance on the basis of terminal force.

Adult↗
PubMed1994

Cutaneous neural codes for shape.

In the pursuit of peripheral neural representations of shape for the sense of touch, a series of two- and three-dimensional objects were stroked across the fingerpad of the anesthetized monkey and responses evoked in cutaneous mechanoreceptive primary afferent nerve fibers recorded. Responses of slowly adapting fibers (SAs) and rapidly adapting fibers (RAs) were recorded to the stroking of a cylinder, a sphere, several ellipsoids, and a pattern of alternating convex and concave cylindrical bars. The compressional force was maintained constant during a stroke, and the stroke velocities as well as orientations of the objects and stroke trajectories were varied between separate sets of trials. The major geometrical properties of the shapes were well represented in the spatiotemporal responses of the afferent fiber populations, particularly those of the SAs. Intensive parameters of shapes, such as the magnitude of change in skin curvature produced as a result of contact with the object surface, were encoded in the discharge rates of SAs and RAs, but this neural code was also influenced by changes in stroke velocity. Spatial parameters of shapes such as the extent of contact and the changes in contour that characterize a shape as belonging to a particular category (such as a sphere as opposed to a cylinder) are encoded in the spatially distributed discharge rates of the SA population. This spatial response profile provides a neural code that is probably invariant with moderate changes in the way the object comes in contact with the skin, such as the contact force or the orientation of the object.

Animals↗
PubMed1994

Cultivation of buffalo green monkey kidney cells persistently infected with hepatitis A virus.

Studies were carried out to determine the effect of prolongation of incubation periods, cocultivation with normal buffalo green monkey kidney (BGMK) cells and different concentrations of foetal calf serum (FCS) on the production of hepatitis A virus (HAV) by BGMK cell line persistently infected with HAV strain HM175. HAV could be detected from week 1 onwards. However, maintenance of cultures beyond this period was found to yield substantially higher quantities of virus. Cocultivation of persistently infected cells with normal BGMK cells also improved the antigen yields. Different concentrations of FCS did not show any effect on the amount of virus produced. The cell line was maintained up to 46 passages during which there was continuous production of HAV in the cells and release of small amounts of virus in the culture supernatants. Cell associated and cell free viral particles were found to be infectious. Supernatant derived virus was a highly suitable inoculum for infecting other susceptible cell lines. Persistently infected BGMK cell line appears to be a reliable and economical source to derive HAV in adequate amounts for diagnostic and research purposes.

Animals↗
PubMed1993

Responses of cutaneous mechanoreceptors to the shape of objects applied to the primate fingerpad.

The present study is one of a series whose aim is to determine how shape is represented in the activity of cutaneous mechanoreceptive peripheral nerve fibers. Cylindrical bars of varying curvature were indented into the receptive fields of slowly and rapidly adapting mechanoreceptive afferent nerve fibers (SAs and RAs respectively) supplying the fingerpad of the anesthetized monkey. The evoked pattern of nerve impulses in single nerve fibers was recorded electrophysiologically. SAs responded to differences in the curvature, both during the ramp and static phases of the skin indentation. RAs responded only during the ramp phase of the indentation, but their responses were not modulated by differences in curvature. Evidence from the present and previous studies is used to support the following hypotheses: Spatial parameters (such as the 'shapes' or 'widths' of responses rates plotted over the skin surface) of primarily SAs in a spatially distributed population of fibers govern the recognition of the overall object shape as a distribution of curvatures; Intensive parameters (such as the magnitude of discharge rates) of only SAs under static indentations, and both SAs and RAs under stroking, are important for discriminations of small differences in curvatures of objects belonging to the same category of shape.

Afferent Pathways↗
PubMed1993

Hepatitis E virus infection in pregnant rhesus monkeys.

Ten non-pregnant female monkeys and four pregnant monkeys (all Macaca mulatta) in the last third of their gestation period were infected intravenously with the stool sample of a patient with hepatitis E virus infection (immuno-electronmicroscopy positive for hepatitis E virus). Four more non-pregnant monkeys were inoculated with a lower dose (less number of virus particles by IEM) of a stool sample collected on a different day from the same patient. The average incubation period as evidenced by the rise of serum alanine transferase in the non-pregnant monkeys, was 36.4 +/- 4.9 days. The dose of the virus did not affect the incubation period. Two of the pregnant monkeys had incubation periods of 9 and 13 days respectively. They delivered healthy babies on 40th and 53rd day respectively after inoculation. At the age of 11 months, both babies were negative for anti-HEV antibodies. One monkey which delivered a healthy baby on the 2nd day after inoculation had incubation period of 36 days. The baby of this monkey was anti-HEV positive at the age of 11 months. The incubation period was 41 days in the fourth monkey which delivered a macerated foetus on the 36th day after infection. No fatality was recorded in the infected monkeys. Bile samples collected from all monkeys showed strong signals in nested polymerase chain reaction (PCR). It seems that the incubation period in pregnant monkeys was determined by the state of pregnancy.

Alanine Transaminase↗
PubMed1991

Observations on respiratory and cardiovascular rhythmicities during yogic high-frequency respiration.

Yogic high-frequency respiration--kapalabhati (KB)--was studied in 24 subjects from a point of rhythmicity. Respiratory movements, blood pressure and R-R intervals of ECG were recorded in parallel and evaluated by spectral analysis of time series. Respiratory signals during KB were modulated by 0.1 Hz rhythm in 82% of experiments. This component was also present in R-R intervals and blood pressure during KB. Frequency (0.2-0.3 Hz) was observed in 67% of respiratory records. The presence of the component 0.2-0.3 Hz in respiration was dependent on resting respiratory frequency. This frequency component was reduced in R-R intervals but increased in blood pressure during kapalabhati as compared to that at rest. The occurrence of both frequency components in respiration during KB supports the hypothesis about the integrative role of cardiovascular and respiratory rhythms in physiological states characterized by altered respiratory frequency.

Adult↗
PubMed1990

Tactile detection of slip: surface microgeometry and peripheral neural codes.

1. The role of the microgeometry of planar surfaces in the detection of sliding of the surfaces on human and monkey fingerpads was investigated. By the use of a servo-controlled tactile stimulator to press and stroke glass plates on passive fingerpads of human subjects, the ability of humans to discriminate the direction of skin stretch caused by friction and to detect the sliding motion (slip) of the plates with or without micrometer-sized surface features was determined. To identify the associated peripheral neural codes, evoked responses to the same stimuli were recorded from single, low-threshold mechanoreceptive afferent fibers innervating the fingerpads of anesthetized macaque monkeys. 2. Humans could not detect the slip of a smooth glass plate on the fingerpad. However, the direction of skin stretch was perceived based on the information conveyed by the slowly adapting afferents that respond differentially to the stretch directions. Whereas the direction of skin stretch signaled the direction of impending slip, the perception of relative motion between the plate and the finger required the existence of detectable surface features. 3. Barely detectable micrometer-sized protrusions on smooth surfaces led to the detection of slip of these surfaces, because of the exclusive activation of rapidly adapting fibers of either the Meissner (RA) or the Pacinian (PC) type to specific geometries of the microfeatures. The motion of a smooth plate with a very small single raised dot (4 microns high, 550 microns diam) caused the sequential activation of neighboring RAs along the dot path, thus providing a reliable spatiotemporal code. The stroking of the plate with a fine homogeneous texture composed of a matrix of dots (1 microns high, 50 microns diam, and spaced at 100 microns center-to-center) induced vibrations in the fingerpad that activated only the PCs and resulted in an intensive code. 4. The results show that surprisingly small features on smooth surfaces are detected by humans and lead to the detection of slip of these surfaces, with the geometry of the microfeatures governing the associated neural codes. When the surface features are of sizes greater than the response thresholds of all the receptors, redundant spatiotemporal and intensive information is available for the detection of slip.

Animals↗
PubMed1989

Surface deflection of primate fingertip under line load.

A study of the biomechanics of the skin and the subcutaneous soft tissues is of fundamental importance in understanding the process of transduction at the mechanoreceptive nerve terminals responsible for the sense of touch. In the present investigation, the fingertips (distal phalanges) of three adult humans and four monkeys were indented in vivo using a line load delivered by a sharp wedge. The resulting skin surface deflection profile was photographed and used as a clue to infer the mechanical nature of the materials that make up the fingertip. It is shown that the modified Boussinesq solution used by Phillips and Johnson (1981), applicable when the fingertip is modeled as an elastic half-space in a state of plane strain, predicts a skin surface deflection profile that can only roughly approximate the empirically observed profiles. As an alternative, a simple model which views the fingertip as an elastic membrane filled with an incompressible fluid (like a 'waterbed') under plane strain conditions is proposed. It is shown that the predictions of this model, which takes into account the finite deformations that occur, agree very well with the photographed profiles in the region of interest (up to about 3 mm from the load).

Adult↗
PubMed1987

Tactile discrimination of shape: responses of slowly adapting mechanoreceptor afferents to a step stroked across the monkey fingerpad.

The representation of shape in the responses of slowly adapting mechanoreceptive afferent fibers (SAs) in monkeys was investigated. A series of flat plates was used, each having an increase in thickness (a step) in the middle so that one-half of the plate was thicker than the other. The cross-sectional shape of the step approximated that of a half-cycle of a sinusoid. The height of the step was fixed at 0.5 mm, while its width (half-cycle wavelength) was varied from 0 to 3.13 mm, resulting in step shapes that varied in steepness and curvature. The steps fell into 2 categories, characterized as "steep" and "gradual." A servocontrolled mechanical stimulator stroked each step across the distal fingerpad from the high to the low side of the step and back, while maintaining the contact force at 20 gm wt. Evoked action potentials in single SAs innervating the fingerpads of anesthetized monkeys were recorded. Each SA's response to a step provided a spatial response profile (discharge rate as a function of step position) that reflected the distribution of curvature across the step shape. All the major features of the SA response could be consistently explained as being due to the sensitivity of the SA to the amount and rate of change in skin curvature. The response profile was altered by changes in stroke direction, step shape, and stroke velocity. Differences in stroke direction (back and forth) were indicated by differences in pattern of response: a "burst-pause-burst" for strokes from high to low, and a "pause-burst-pause" for strokes from low to high; a greater discharge rate in response to the step for low to high strokes, and for some SAs, the reduction or absence of basal discharge in one of the directions. The discharge rate during the burst for either direction of stroking was greater for steep than for gradual steps, and increased, for a given step shape, with increases in stroke velocity. Regardless of differences in stroke velocity, steep steps were distinguished from gradual steps by having narrower burst widths for low-to-high strokes and narrower pause widths for high-to-low strokes. The same stimuli were delivered to the human fingerpad, and the capacities of humans to discriminate between the steps were measured. It was concluded that the spatial features of SA responses, representing the widths of regions of active and inactive SA populations, as well as the intensive feature of discharge rate, accounted for the gross sensory discriminations of shape.(ABSTRACT TRUNCATED AT 400 WORDS)

Adaptation, Physiological↗
PubMed1987

Tactile discrimination of shape: responses of rapidly adapting mechanoreceptive afferents to a step stroked across the monkey fingerpad.

Responses of rapidly adapting Meissner corpuscle mechanoreceptive afferent fibers (RAs) to steps of varying shape stroked across the distal fingerpad were recorded from anesthetized monkeys. A series of flat plates were used, each having an increase in thickness (a step) in the middle so that one-half of the plate was thicker than the other. The cross-sectional shape of the step approximated that of a half-cycle sinusoid, 0.5 mm high. The width (half-cycle wavelength) of the sinusoidal step was varied from 0 to 3.13 mm, producing a series of step shapes that differed in steepness and curvature. These steps could be broadly categorized into 2 groups, "steep" and "gradual." Each step was stroked back and forth under constant compressional force, using a servocontrolled mechanical stimulator. The RA's response to a step provided a spatial pattern of action potentials in which the occurrence of each impulse corresponded to a position of the step on the skin. This response consisted of a single "burst" of impulses to the sinusoidal portion of the step. Changes in stroke direction, step shape, or velocity of stroking primarily affected the RA discharge rate during the burst, and, less consistently, the spatial width of the burst. For a given step shape and stroke velocity, the discharge rate was greater for strokes from the low to the high side of the step than for strokes from the high to the low side. Discharge rate was greater for steep than for gradual steps and, for a given step, it increased with stroke velocity. All the major features of the responses were interpreted as being due predominantly to the sensitivity of the RA to vertical velocity at the most sensitive spot on its receptive field, together with a sensitivity to the rate of change in skin curvature at that spot. RA discharge rate distinguished not only the gross differences between steep and gradual steps, but also some of the finer differences in sharpness among steep steps. From a comparison with the human capacity for tactile discrimination of the steps, it was concluded that RAs, through their discharge rates, provide primarily "intensive" information about the sharpness of shapes.

Adaptation, Physiological↗