To assess the possibilities of using 114mIn as a therapeutic agent, the long-term biodistribution of 114mIn was studied, in tumor-bearing nude mice, after injection of labeled monoclonal antibody (MoAb) B72.3 IgG, either DTPA-coupled murine, DTPA-coupled chimeric, or macrocycle-coupled chimeric antibody. Although the biodistributions in all cases were similar, there were important differences. The use of DTPA-coupled chimeric antibody led to higher concentrations of radioactivity in tumor, and to lower concentrations in liver and bone, as compared to DTPA-coupled murine antibody. The use of macrocycle-coupled chimeric antibody led to higher concentrations of radioactivity in the liver and in bone as compared to the DTPA-coupled chimeric antibody. However, in this case there were no significant differences in tumor uptake or clearance. Radiation doses were calculated based on the organ retention and by neglecting source-to-target contributions. Radiation dose distribution was marginally favorable for therapy in the group injected with DTPA-coupled chimeric antibody.

Animals↗

PubMedDate not supplied

Changes in renin and blood pressure levels during renal transplantation.

Early in the transplant program we experienced difficulty in maintaining the blood pressure after bilateral nephrectomy of a proportion of recipients receiving kidneys from living donors. To our surprise this problem was much less marked in the less prepared recipients with cadaver donors. It seemed possible that this could be related to our practice of preceeding transplantation with bilateral nephrectomy in the same session when the donor was alive, but not after necrotransplantation. A typical blood pressure chart from an operation combining renal transplantation with bilateral nephrectomy is shown in fig. 1. Even where there is no blood loss the systolic and diastolic blood pressure falls with the central venous pressure so that perfusion of the donor kidney is at first poor. As soon as perfusion is established however, the blood pressure rises.

Adolescent↗