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Biomedical subjects

M Abdel-Salam

Publications and source records attributed to M Abdel-Salam.

6 recordsLinked to original sources
PubMed1995

Transmission-line electric field induction in humans using charge simulation method.

This paper is aimed at determining the distribution of the fields, induced charges, and currents on a human body standing in the high electric fields produced by high voltage overhead transmission lines. This method of analysis is based on the charge simulation technique. This will serve to explain the biological studies of possible long-term exposure effects to electric fields.

Data Interpretation, Statistical↗
PubMed1990

Which oral white lesions will become malignant? An image cytometric study.

We investigated the value of image cytometry in predicting the prognosis of oral epithelial lesions, whether or not they show dysplasia. Thirty-five oral epithelial lesions were studied retrospectively. Of these, 23 had later transformed to carcinoma and 12 had not. By means of the Leitz TAS image analyzer, 200 nuclei of epithelial cells and 20 nuclei of lymphocytes from each section were individually assessed for eight features related to shape and amount of stain and for six features related to chromatin pattern. The mean, standard deviation, and interquartile range of each feature were calculated, first for each section and then for each group. With the use of linear stepwise discriminant analysis we constructed a predictive model, which consisted of three variables related to chromatin pattern. The variables were mean margination, standard deviation of clumping, and standard deviation of condensation. In the jackknife classification, this model predicted the malignant potential of the lesions that later transformed to cancer with 86% predictive value and 83% sensitivity.

Adult↗
PubMed1988

Prediction of malignant transformation in oral epithelial lesions by image cytometry.

The value of image analysis in predicting the malignant potential of oral epithelial lesions showing either hyperplasia or dysplasia was investigated; 5-micron formalin-fixed sections of 16 oral epithelial lesions, of which eight had later transformed to carcinoma and eight had not transformed during a follow-up of 10-15 years were studied. The sections were stained with the azure A-Feulgen reaction for nuclear DNA. In each section 200 nuclei of epithelial cells and 20 nuclei of lymphocytes were assessed; all measurements were made blindly. For each nucleus six features related to shape and amount of stain and six features related to chromatin pattern were assessed. For each feature the mean, SD, and interquartile range were determined and used for linear stepwise discriminant analysis. A model of three variables with the most discriminating power was developed. When the jackknifed classification test was applied using this model, the malignant potential of the lesions that later transformed could be predicted with 87.5% accuracy.

Aged↗
PubMed1987

Nuclear DNA analysis of oral hyperplasia and dysplasia using image cytometry.

We investigated the value of image analysis in discriminating among oral white lesions with hyperplasia without dysplasia and oral white or white-and-red lesions with moderate or severe dysplasia. Normal oral epithelial tissue was used as a control. Image analysis was applied to 5-micron formalin-fixed sections stained with the azure A-Feulgen reaction for nuclear DNA. For 150-200 cells from each section, 5 nuclear variables were assessed: area, form factor, total stain, average stain and ellipticity. For each variable, 2 measurements were obtained, the mean and the interquartile range, and were used for stepwise discriminant analysis. Using this test, a model of 3 measurements with the most discriminating power was developed. When the jackknife classification test was applied to this model, we could discriminate with 81% accuracy between the 4 groups of tissue studied.

Carcinoma, Squamous Cell↗
PubMed1984

Characterization of oral streptococci that activate the dietary glycoside rutin to a mutagen.

Oral streptococci are described that hydrolyze the dietary glycoside rutin (CAS: 153-18-4), resulting in mutagenic activity. Bacteria that hydrolyze rutin were isolated from the mouth of each of 10 healthy volunteers. The activity was inducible, and the product was mutagenic in the Salmonella mutagenicity assay. These bacteria were present in the greatest proportion on the dorsum of the tongue where they formed 1.5% of the total cultivable microflora. The appearance of the colonies, cell morphology, and biochemical characteristics were those of Streptococcus milleri. However, rutin hydrolysis did not occur with any of 30 isolates of this species from blood of patients with bacteremia, with any of 4 stock strains, or with any of 14 strains of streptococci from the other 4 major oral species. Therefore, activation of rutin to a mutagen is not a universal function of the normal oral flora, but it can be accomplished by some strains of S. milleri.

Biotransformation↗