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Biomedical subjects

M Ajiki

Publications and source records attributed to M Ajiki.

13 recordsLinked to original sources

[Hepatic arteriography under temporary hepatic venous occlusion].

Hepatic arteriography with and without temporary segmental hepatic vein occlusion was performed in 10 patients, five of whom had chronic liver injury. Hepatic arteriograms obtained during hepatic venous obstruction demonstrated significantly more peripheral and definite arterial branches in the occluded area and fewer peripheral branches in the non-occluded segment. A prolonged, dense hepatogram (sinusoidogram) showing hepatofugal opacification of the portal vein was obtained in the occluded area. Only one case with a large veno-venous anastomosis did not show these findings. Hepatic arteriograms in two cases with hepatocellular carcinoma provided clear visualization of peripheral portal branches that could act as efferent tumor vessels during regional temporary hepatic vein occlusion. Temporary hepatic venous occlusion may cause a sudden increase of hepatic arterial flow in the occluded area and transsinusoidal arterioportal communication there. This method can be useful for the diagnosis and arterial infusion or embolization therapy of hepatic diseases.

Adult

Comparison of feline parvovirus subspecific strains using monoclonal antibodies against a feline panleukopenia virus.

Four monoclonal antibodies (mAb) against a feline panleukopenia virus (FPLV) TU 1 strain, one of the host range variants of feline parvovirus (FPV), were produced and applied for antigenic analysis of FPLV, canine parvovirus (CPV) and mink enteritis virus (MEV). All mAbs were considered to be directed at epitopes on the virus capsid surface because they neutralized the infectivity and inhibited the hemagglutination (HA) of the homologous virus as well as other FPV strains. They were of the mouse IgG1 type. High antigenic homogeneity among FPLV strains was confirmed by HA-inhibition (HI) test with the mAbs and polyclonal immune sera against FPLV or CPV. But the TU 11 strain of FPLV was antigenically distinguished from the remaining 14 FPLV strains by both the HI test and the micro-neutralization test with one of the mAbs produced. MEV Abashiri strain was found to be antigenically indistinguishable from FPLV. Most of the CPV strains isolated after 1981 were considered to be antigenically different from earlier CPV isolates when some mAbs were applied in the serological tests, confirming the replacement of CPV by an antigenic variant in Japan. However, antigenically different CPVs were detected at the end of 1984 from unrelated epizootics occurred a month apart in the same area.

Animals

Infectivity for chicken embryos of tissue-culture-modified infectious laryngotracheitis virus.

The present study shows the infectivity for chicken embryos of the SPL strain of tissue-culture-modified infectious laryngotracheitis (ILT) virus. The modified virus formed small pocks on the chorioallantoic membrane (CAM) and gave no adverse reaction after administration to chickens. For 1- and 6-day-old embryonated specific-pathogen-free (SPF) eggs, the SPL strain showed higher infectivity than virulent strain NS-175 or wild strains of ILT virus. A high percentage of embryos of day-old eggs inoculated with strains SPL via the yolk sac died within several days, and virus was recovered from yolk and embryos with high titer. In the embryos inoculated at 6 days of age, histopathological lesions characterized by syncytium formation with intranuclear inclusion bodies were observed mainly in the CAM, liver cells, epithelial cells of the oral cavity, and esophagus and rarely in epithelial cells of the uriniferous tubule, bronchus, trachea, choroid plexus, or feather follicles. In the 10-day-old embryonated SPF eggs inoculated via the chorioallantoic cavity, however, the SPL strain demonstrated low pathogenicity compared with strain NS-175 and the two wild strains.

Animals