Biomedical subjects
M Alberti
Publications and source records attributed to M Alberti.
Structural analysis of c-myc in human sporadic colorectal carcinomas.
Analysis of the DNA extracted from the colonic mucosa of 13 patients operated for colorectal carcinoma revealed c-myc rearrangements in three of them. In all cases, the alterations were found both in the histologically normal and in the tumoral mucosa. In one case, a different pattern of rearrangements characterized the two tissues. In all three individuals, the c-myc sequences appeared to be amplified. We suggest that c-myc rearrangements in the histologically normal colonic mucosa of colorectal carcinoma patients could represent a predisposition factor for the development of additional neoplasms.
Optimization of RNA and DNA purification procedures from human colorectal carcinomas.
We optimized procedures to extract RNA and DNA separately from colorectal carcinomas pulverized by Microdismembrator II. Our procedures make it possible to achieve good yields and high degree of purification of both types of macromolecules without affecting their structural integrity.