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Biomedical subjects

M Arashi

Publications and source records attributed to M Arashi.

10 recordsLinked to original sources
PubMed1989

Chemotactic properties of rat immunoglobulins and immune complexes.

The effect of rat immunoglobulins and immune complexes on the locomotor function of rat polymorphonuclear leukocytes (PMN) was investigated in vitro. Rat immunoglobulin G1 (IgG1), IgG2a, IgG2b, and IgA monoclonal antibodies specific for the dinitrophenyl hapten were used. Both monomeric and polymeric IgA showed chemotactic activity in a dose-dependent manner. IgG1 and IgG2b also induced a dose-dependent locomotor response of PMN, but the nature of the induced migration was chemokinetic (enhancing random migration). IgG2a was chemotactic and induced maximal migration at a relatively low concentration. IgG1- and IgG2b-immune complexes induced stronger migration than antibody alone; however, IgA- and IgG2a-immune complexes did not. IgA was shown to modify the chemotactic movement of PMN induced by N-formylmethionyl-leucyl-phenylalanine (FMLP). In the presence of both IgA and FMLP in the lower chamber, the migration towards suboptimal concentrations of FMLP was enhanced. By contrast, IgA in the upper chamber decreased migration towards the optimal or higher concentrations of FMLP. These findings suggest that IgA may work synergistically with luminal chemoattractants to mobilize PMN to the locus of infection on the mucosal surface. In addition, the intense activity of IgG2a alone and IgG1- or IgG2b-immune complexes in inducing PMN migration may play an important role in inflammatory processes. The data indicate that immunoglobulins have a direct effect on PMN mobility.

Animals↗
PubMed1987

Molecular forms of IgA produced by various lymphoid tissues--analysis using high speed liquid chromatography.

Molecular forms of immunoglobulin A (IgA) produced by cultured cells from various human lymphoid tissues were analyzed using high speed liquid chromatography (HLC). IgA secreted into culture media was easily separated into polymeric and monomeric forms by HLC. HLC has the advantages of high resolution, reproducibility, rapidity and technical simplicity in the separation of polymeric and monomeric IgA. Peripheral blood lymphocytes and cells from gut-associated lymphoid tissues, such as mesenteric lymph nodes or large bowel mucosa, secreted predominantly polymeric IgA, whereas lymphoid cells from bone marrow produced mainly monomeric IgA. Spleen cells and tonsillar cells produced nearly equal proportions of polymeric and monomeric IgA. These results suggest that with regard to IgA in serum, the polymer may originate from the gut-associated lymphoid tissues and the monomer may mostly derive from the bone marrow.

Adult↗
PubMed1983

Distribution of secretory component in non-cancerous human gastric mucosa.

Immunological detection of secretory component (SC) in non-cancerous human gastric mucosa was carried out by the peroxidase-anti-peroxidase (PAP) method using several fixatives. SC was detected in the generative zone or mucous neck of normal gastric mucosa without intestinal metaplasia (IM) and in absorptive cells of IM of the stomach. The deep part of complete type IM showed a larger amount of SC than the superficial part. These findings suggested that the appearance of SC in gastric mucosa would be relevant to the immunity of glandular epithelium. In addition, SC was also detected in pyloric gland cells in specimens fixed with cold 95% ethanol. From these results, SC was considered to be a normal constituent and one of the developmental proteins in the gastric mucosa.

Gastric Mucosa↗