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M Ardehali

Publications and source records attributed to M Ardehali.

14 recordsLinked to original sources
PubMed1986

Mass production and standardization of Clostridium oedematiens vaccine against black disease (infectious necrotic hepatitis) of sheep.

The object of this study was to prepare a potent vaccine against black disease of sheep. Attempts were made to prepare and formulate the ingredients in order to obtain high yield of toxin. A 600 litre batch of mass production of Clostridium oedematiens (Cl. novyi) vaccine was prepared with ingredients consisting of 4% peptone, 0.25% NaCl, 0.5% liver extract, 0.1% L-cysteine, 1% maltose, 0.02% sodium dithionite and 0.25% ferrous sulphate solution. The prepared vaccine was diluted to concentrations of 20, 40, 60 and 80 per cent of antigens. Potassium alum was added as an adjuvant. The potency test of the prepared vaccine was determined in a group of forty rabbits according to the British Pharmacopoeia (Veterinary). Maximum titre was obtained at 80 per cent with the level of 33 units per ml of alpha antitoxin in rabbits pooled serum. 20, 16 and 8 units per ml of alpha antitoxin were obtained respectively for 60, 40 and 20 per cent of diluted antigen in rabbits pooled serum. Sheep have been vaccinated in black disease areas with this vaccine. Reports obtained from the field indicate that black disease in sheep could be effectively controlled by this vaccine in Iran.

Animals↗
PubMed1976

Production and standardization of polyvalent Clostridium perfringens vaccine in Iran.

In this presentation an account of the large-scale production and the standardization of polyvalent Clostridium perfringens vaccine in Iran is described. Over 20 million doses of this vaccine are produced and utilized in Iran every year. Certain modifications have been made to the culture media used in our laboratory for the production of this vaccine in order to bring down its cost. The prepared vaccine is highly immunogenic as determined by the laboratory examination on the quality of the vaccine according to British Veterinary Codex and the field reports.

Animals↗