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M Argirova

Publications and source records attributed to M Argirova.

8 recordsLinked to original sources
PubMed2000

Mass spectrometric monitoring of albumin in uremia.

BACKGROUND: Advanced glycation end products (AGEs) are a novel class of uremic toxins. In plasma, they are present in proteins and also in low molecular mass peptides. AGE-modified peptides are thought to bind and modify plasma proteins. Monitoring of the consequent increase in molecular mass of serum albumin may be used in surveillance of the clinical management of uremia. METHODS: We investigated molecular mass changes of human serum albumin (HSA) glycated by glucose and methylglyoxal in vitro and of subjects with moderate renal impairment, end-stage renal disease (ESRD), ESRD on hemodialysis, and normal healthy controls by matrix-assisted laser desorption ionization mass spectrometry. RESULTS: Fatty acid-free HSA had a molecular mass of 66,446 +/- 114 D. Mean (+/-SD) molecular mass increases were HSA minimally glycated by glucose 399 +/- 88 D (N = 5, P < 0.001), HSA highly glycated by glucose 6780 +/- 122 D (N = 5, P < 0.001), HSA minimally glycated by methylglyoxal 73 +/- 121 D (N = 5, P > 0.05), and HSA without fatty acid removal 535 +/- 90 D (N = 5, P < 0.001). For HSA of human subjects, mean (+/- SD) molecular mass increases were normal healthy controls 243 +/- 97 D (N = 5), moderate renal impairment 350 +/- 83 D (P > 0.05 with respect to controls, N = 5), ESRD 498 +/- 128 (P < 0.02 with respect to controls, N = 3), and ESRD on hemodialysis 438 +/- 85 D (P < 0.02 with respect to controls, N = 5). The mean molecular mass of albumin of all groups was increased significantly with respect to that of fatty acid free albumin (P < 0.001). CONCLUSIONS: Only ESRD was associated with a significant increase in the molecular mass of HSA in vivo. Since this mass increase was very low and much lower than reported for AGE-modified peptides, it may reflect AGE formation on HSA by alpha-oxoaldehydes that accumulate in uremia, rather than modification of albumin by AGE-modified peptides. The molecular mass of HSA in vivo was indicative of a minimal and not a high extent of glycation.

Adult↗
PubMed1988

[An attempt at the dynamic classification of population centers in Bulgaria in relation to atmospheric pollution].

A method for evaluation, comparison and classification of the settlements is proposed according to the rate of pollution of the atmospheric air by a given component. For basis is accepted the sigma method. Three normative categories for classification are used. It is proceeded from the standardized towards MAC concentrations. In this case the average annual are applied. The method permits an objective comparison and classification in accordance with the rate of pollution of the settlements and their survey in dynamics. The approbation is made on the basis of the data from 1981 and 1985 for the settlements with 5 pollutants. It is established that the basic group of settlements do not change their category during the 5-year plan, while the changes of the groups are smooth in the neighbouring categories. The method gives adequate results and could serve the evaluation of the state of the atmospheric air in this country and the effectiveness of the air protective measurements.

Air Pollutants↗