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M B Perry

Publications and source records attributed to M B Perry.

At least 91 records · Page 5Linked to original sources
PubMed1992

Characterization of the lipopolysaccharide O antigens of Actinobacillus pleuropneumoniae serotypes 9 and 11: antigenic relationships among serotypes 9, 11, and 1.

The antigenic lipopolysaccharide O polysaccharides of capsular serotypes 9 and 11 were examined by chemical, immunological, and nuclear magnetic resonance methods. Immunodiffusion tests carried out on these O antigens indicated that both contained common epitopes which were also shared by Actinobacillus pleuropneumoniae serotype 1. Chemical analysis and high-field nuclear magnetic resonance spectroscopy showed that the O antigens of serotypes 9 and 11 were high-molecular-weight polymers consisting of a backbone of repeating trisaccharide units composed of alpha-L-rhamnopyranosyl and alpha-D-glucopyranosyl residues (2:1). One of the alpha-L-rhamnose units forms a branch point and is stoichiometrically substituted with terminal 2-acetamido-2-deoxy-beta-D-glucose residues in the serotype 11 O polysaccharide, but only to the extent of 25% in the serotype 9 O polysaccharide. Thus, the serotype 9 O polysaccharide contains two different repeating units: a tetrasaccharide unit with the same structure as that of the serotype 11 O polysaccharide and a trisaccharide unit: [formula: see text] where R = beta-D-GlcpNAc for serotype 1 and 11 O polysaccharides, and R = H (75%) and R = beta-D-GlcpNAc (25%) for serotype 9. The structure of the previously determined serotype 1 O polysaccharide (E. Altman, J.-R. Brisson, and M. B. Perry, Biochem. Cell. Biol. 64:17-25, 1986) is identical to that of the serotype 11 O polysaccharide. We propose a more complete serotyping scheme for A. pleuropneumoniae which includes designation of both the capsular (K) and O antigens.

Actinobacillus pleuropneumoniae↗
PubMed1992

Capsule structure of Proteus mirabilis (ATCC 49565).

Proteus mirabilis 2573 (ATCC 49565) produces an acidic capsular polysaccharide which was shown from glycose analysis, carboxyl reduction, methylation, periodate oxidation, and the application of one dimensional and two-dimensional high-resolution nuclear magnetic resonance techniques to be a high-molecular-weight polymer of branched trisaccharide units composed of 2-acetamido-2-deoxy-D-glucose (N-acetyl-D-glucosamine), 2-acetamido-2,6-dideoxy-L-galactose (N-acetyl-L-fucosamine), and D-glucuronic acid, having the structure: [formula: see text] P. mirabilis 2573 also produces an O:6 serotype lipopolysaccharide in which the O-chain component has the same structure as the homologous capsular polysaccharide. This is the first report of a defined capsular polysaccharide in this bacterial genus.

Carbohydrate Sequence↗
PubMed1992

Structural characterization of the O-polysaccharide of the lipopolysaccharide produced by Salmonella milwaukee O:43 (group U) which possesses human blood group B activity.

The O-polysaccharide of the lipopolysaccharide produced by Salmonella milwaukee O:43 (group U) was shown by composition analysis, methylation, periodate oxidation, and 1H and 13C nuclear magnetic resonance spectroscopic analytical methods to be a polymer of branched pentasaccharide repeating units having the structure: [formula: see text] The blood-group activity of the O-polysaccharide was established by its serological reactivity with a specific monoclonal antibody to human blood group B, using passive hemagglutination and ELISA assays, indicating the common antigenic epitope to be a nonreducing terminal trisaccharide unit composed of L-Fucp and D-Galp (1:2) residues.

ABO Blood-Group System↗
PubMed1991

Structure of the capsular polysaccharide from Actinobacillus pleuropneumoniae serotype 10.

The capsular polysaccharide of A. pleuropneumoniae serotype 10 was found to be composed of a linear disaccharide repeating unit. By use of methylation analysis, partial hydrolysis, g.l.c.--and f.a.b.- m.s., and 1D and 2D n.m.r. studies, the polysaccharide was determined to be a polymer of a repeating disaccharide unit composed of D-mannose and 3-deoxy-D-manno-octulosonic acid (Kdo) having the structure: [formula; see text]

Actinobacillus pleuropneumoniae↗
PubMed1991

Structural studies of the capsular polysaccharide from Actinobacillus pleuropneumoniae serotype 12.

The capsular polysaccharide of A. pleuropneumoniae is composed of 2-acetamido-2-deoxy-D-glucose (3 parts) and phosphate (1 part). The arrangement of these components in the repeating unit was determined by dephosphorylation, methylation, g.l.c.-m.x., and 1D and 2D n.m.r. spectroscopic methods. The polysaccharide was found to be a high molecular weight polymer of repeating trisaccharide units, joined through phosphate diester linkages, having the structure, (formula: see text).

Actinobacillus pleuropneumoniae↗
PubMed1991

Differences between the LPS cores in adherent and non-adherent strains of enteropathogenic Escherichia coli 0119.

Adherent enteropathogenic Escherichia coli 0119 strains had a larger lipopolysaccharide core than non-adherent strains, although the O-chains were identical. The core from the non-adherent strain 19392 contained five hexose residues in the outer region, with three L-glycero-D-manno-heptose residues and 3-deoxy-D-manno-octulosonic acid (KDO) in the inner region. The core of adherent strain JCP88 had an atypical structure consisting of six hexose residues, KDO, and equimolar amounts of L-glycero-D-manno-heptose and D-glycero-D-manno-heptose. The core of a rough JCP88 mutant resembled an incomplete 19392 core.

Bacterial Adhesion↗
PubMed1991

Structure of the capsular polysaccharide from Actinobacillus pleuropneumoniae serotype 7.

The structure of the capsular polysaccharide antigen of A. pleuropneumoniae serotype 7 was determined using 1D- and 2D-n.m.r. methods. Dephosphorylation, methylation, and g.l.c.-mass spectrometry methods were used to confirm the analysis. The type-7 specific polysaccharide was a high-molecular-weight teichoic acid-type polymer of D-galactose, glycerol, and phosphate (2:1:1), composed of glycosylglycerol repeating units joined through monophosphate diester linkages and with the structure: [formula: see text]

Actinobacillus↗
PubMed1991

Expression of two structurally distinct D-galactan O antigens in the lipopolysaccharide of Klebsiella pneumoniae serotype O1.

The lipopolysaccharide (LPS) molecule is an important virulence determinant in Klebsiella pneumoniae. Studies on the serotype O1 LPS were initiated to determine the basis for antigenic heterogeneity previously observed in the O1 side chain polysaccharides and to resolve apparent ambiguities in the reported polysaccharide structure. Detailed chemical analysis, involving methylation and 1H- and 13C-nuclear magnetic resonance studies, demonstrated that the O-side chain polysaccharides of serotype O1 LPS contained a mixture of two structurally distinct D-galactan polymers. The repeating unit structures of these two polymers were identified as [----3)-beta-D-Galf-(1----3)-alpha-D-Galp-(1----] (D-galactan I) and [----3)-alpha-D-Galp-(1----3)-beta-D-Galp-(1----] (D-Galactan II). D-Galactan I polysaccharides were heterogeneous in size and were detected throughout the sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis (PAGE) profile of O1 LPS. In contrast, D-galactan II was confined to the higher-molecular-weight region. The structures of the two D-galactans were not influenced by simultaneous synthesis of a capsular K antigen. Apparently, neither of the D-galactans constitutes a common antigen widespread in Klebsiella spp. as determined by immunochemical analysis. Examination of the LPSs in mutants indicated that expression of D-galactan I can occur independently of D-galactan II. Transconjugants of Escherichia coli K-12 strains carrying the his region of K. pneumoniae were constructed by chromosome mobilization with RP4::mini-Mu. In these transconjugants, the O antigen encoded by the his-linked rfb locus was determined to be D-galactan I, suggesting that genes involved in the expression of D-galactan II are not closely linked to the rfb cluster.

Antigens, Bacterial↗
PubMed1991

Characterisation of the Salmonella O:8 antigen in the O-chain of the lipopolysaccharide produced by Salmonella virginia O:8.

The antigenic O-polysaccharide of the lipopolysaccharide produced by Salmonella virginia (O:8), analyzed by methylation, partial acid hydrolysis, and one- and two-dimensional nuclear magnetic resonance methods, was found to be a polymer of a repeating pentasaccharide unit composed of D-mannose, D-galactose, L-rhamnose, D-abequose, and O-acetyl (2:1:1:1:1.3) and having the following structure: [formula; see text] The disaccharide structure alpha-D-Abep-(1----3)-L-Rhap was identified as the Salmonella O:8 antigenic factor epitope, since the removal of alpha-D-Abep residues from the O-polysaccharide left a residual tetrasaccharide repeating unit backbone that did not show reaction with Salmonella type O:8 factor antiserum.

Carbohydrate Conformation↗
PubMed1990

Antigenic relationships of the lipopolysaccharides of Escherichia hermannii strains with those of Escherichia coli O157:H7, Brucella melitensis, and Brucella abortus.

Clinical isolates of Escherichia hermannii which showed serological cross-reaction with polyclonal antisera to the O-polysaccharide portion of the lipopolysaccharide of E. coli O157 strains and with antisera to the O antigens of Brucella abortus and B. melitensis were found by chemical and nuclear magnetic resonance analyses to have lipopolysaccharide O chains composed of linear polymers containing 1,2- and 1,3-linked 4-acetamido-4,6-dideoxy-alpha-D-mannopyranosyl (alpha-D-Rhap4NAc) residues. Two O-antigen structures were identified; each had an unbranched pentasaccharide repeating unit, and one was composed of three 1,2- and two 1,3-linked alpha-D-Rhap4NAc residues and the other had two 1,2- and three 1,3-linked alpha-D-Rhap4NAc residues. The above-described cross-serological reactivities, which have led to false-positive identifications, are related to the common occurrence of epitopes involving the presence of N-acyl derivatives of 4-amino-4,6-dideoxy-D-mannopyranosyl residues in the O-polysaccharide portions of the respective lipopolysaccharides of the organisms. Strains of E. hermannii which did not show serological cross-reactions with E. coli O157 and Brucella antisera were found to have unique lipopolysaccharide O chains devoid of D-Rhap4NAc residues, demonstrating the existence of serotypes of E. hermannii that are distinct on the basis of their lipopolysaccharide components.

Antigens, Bacterial↗
PubMed1990

Structural studies of the O-chains of the lipopolysaccharides produced by strains of Actinobacillus (Haemophilus) pleuropneumoniae serotype 5.

The water-phase lipopolysaccharides isolated by phenol-water extraction from the cells of four strains of Actinobacillus pleuropneumoniae serotype 5 (K17, L20, B78-3760, and 81-750) were shown on structural analysis to have O-chain components with the same basic polysaccharide structure of a linear unbranched homopolymer of 1,6-linked beta-D-galactopyranosyl residues, although the linear length of the O-chain varied among different strains. While those of strains B78-3760 and 81-750 were partially O-acetylated, the O-chains of strains K17 and L20 were unsubstituted.

Actinobacillus↗
PubMed1989

Structure of the lipopolysaccharide antigenic O-chain produced by Actinobacillus pleuropneumoniae serotype 4 (ATCC 33 378).

The structure of the antigenic O-polysaccharide part of the S-type lipopolysaccharide produced by Actinobacillus pleuropneumoniae serotype 4 has been determined by periodate oxidation, methylation, partial hydrolysis, and 1H- and 13C-n.m.r. spectroscopy. The O-polysaccharide structure has a branched-tetrasaccharide repeating unit, (----3)-beta-D-Galp-(1----4)-[beta-D-Glcp-(1----3)]-beta-D-GalpNAc- (1----4)-alpha-L-Rhap-(1-)n. The structure resembles that of the lipopolysaccharide O-chain of A. pleuropneumoniae serotype 7, and their common epitopes may account for the apparent serological cross-reactivity observed between the two serotypes when incompletely adsorbed, anticapsular-typing sera are used.

Actinobacillus↗
PubMed1989

Structure of the lipopolysaccharide antigenic O-chain produced by Salmonella ohio (O:6,7).

Salmonella ohio, which belong to Group C1 (0:6,7) of the Kauffmann-White classification system, produces a smooth lipopolysaccharide which by glycose analysis, methylation, deamination, and 1H-n.m.r. studies was shown to have an O-polysaccharide chain composed of a repeating hexasaccharide unit having the structure [----2)-[alpha-D-Glcp-(1----3)]-alpha-D-Manp-(1----2)-alpha-D-M anp- (1----2)-beta-D-Manp-(1----3)-beta-D-GlcpNAc-(1----2)-beta-D-Ma np-(1----]n.

Carbohydrate Sequence↗
PubMed1989

Fine structure of A and M antigens from Brucella biovars.

Brucella A and M epitopes were found on single O-polysaccharide chains of all biotype strains of this species. Lipopolysaccharides from the type and reference strains of five of the six Brucella species, B. abortus, B. melitensis, B. suis, B. canis, and B. neotomae, were extracted and purified. Analysis by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, in conjunction with silver staining and immunoblotting developed by monoclonal antibodies, showed bands characteristic of A, M, or mixed A and M antigens. The A antigen previously described as an exclusively alpha 1,2-linked homopolymer of 4,6-dideoxy-4-formamido-D-mannopyranose was shown by 1H and 13C nuclear magnetic resonance spectroscopy to possess a fine structure consistent with the low-frequency occurrence of alpha 1, 3-linked 4,6-dideoxy-4-formamido-D-mannopyranose residues. This feature was previously attributed only to the M antigen, which is also a homopolymer of the same sugar. B. melitensis biotype 3 and B. suis biotype 4 lipopolysaccharides showed characteristics of mixed A and M antigens. Immunoabsorption of these O polysaccharides on a column of immobilized A-antigen-specific monoclonal antibody enriched polymer chains with A-antigen characteristics but did not eliminate M epitopes. Composite A- and M-antigen characteristics resulted from O polysaccharides in which the frequency of alpha 1,3 linkages, and hence, M-antigen characteristics, varied. All biotypes assigned as A+ M- expressed one or two alpha 1,3-linked residues per polysaccharide O chain. M antigens (M+ A-) also possessed a unique M epitope as well as a tetrasaccharide determinant common to A-antigen structures. B. canis and B. abortus 45/20, both rough strains, expressed low-molecular-weight A antigen.

Antibodies, Monoclonal↗
PubMed1989

Definition of Brucella A and M epitopes by monoclonal typing reagents and synthetic oligosaccharides.

The paradigm that Brucella A and M epitopes are simultaneously expressed on single cells and within one antigen molecule was reinvestigated by using polysaccharide-specific murine monoclonal antibodies. Monoclonal antibodies were generated to the M antigen of Brucella melitensis 16M. Chemically defined lipopolysaccharides and O polysaccharides from Brucella abortus 1119-3, B. melitensis 16M, and Yersinia enterocolitica O:9 were used to dissect the binding profiles of the B. melitensis antibodies and an additional set of antibodies available from a B. abortus fusion experiment. Binding specificities were rationalized in terms of prototype A- and M-antigen structures, an interpretation supported by competitive binding studies with O polysaccharides and synthetic oligosaccharide analogs of the A and M antigens. Three binding patterns were characterized. Antibodies specific for the A antigen required five contiguous alpha 1,2-linked 4,6-dideoxy-4-formamido-D-mannopyranosyl residues, while antibodies with equal affinities for A or M epitopes were effectively inhibited by alpha 1,2-linked tri- or tetrasaccharides. Specificity for the M epitope correlated with binding of a critical disaccharide element alpha-D-Rha4NFo(1----3)alpha-D-Rha4NFo bracketed by alpha 1,2-linked residues. The binding profiles of Brucella monoclonal antibodies were consistent with the concept of simultaneous expression of A and M epitopes within a single molecule. A epitopes were present in the M antigen, and the discovery of isolated alpha 1,3 linkages in the A antigen suggests that M epitopes occur in all A antigens. Three monoclonal antibodies are proposed as standard reagents for the detection and identification of Brucella A and M antigens.

Animals↗