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M B Yarmolinsky

Publications and source records attributed to M B Yarmolinsky.

26 records · Page 2Linked to original sources

Segregation of functional sex factor into minicells.

The segregation of a bacterial plasmid, the sex factor F', has been investigated in a cell-division mutant of Escherichia coli which produces small anucleate cells (minicells). Significant amounts of isotopically labeled DNA segregate into minicells dependent upon the presence of F'. Minicells containing F'Gal or F'(lambda) are shown to donate the plasmid in conjugation. These results demonstrate that the sex factor may be dissociated from the bacterial chromosome and that this separation does not prevent its subsequent transfer.

Carbon Isotopes↗

An upper limit on beta-galactosidase transfer in bacterial conjugation.

An upper limit for beta-galactosidase transfer between mating F(+) and F(-)Escherichia coli has been determined by a new technique which relies on selective lysis of the donor strain by heat induction of a thermo-inducible strain of lambda, accompanied by chymotryptic digestion of the released beta-galactosidase. No significant transfer of beta-galactosidase during mating between F(+) and F(-) cells has been observed: 0.05 +/- 0.05% of the enzyme originally present in the male cells is found in the female cells after 1 hr of mating at 37 C.

Bacteriolysis↗